Investigating the effects of municipal and hospital wastewaters on horizontal gene transfer

Hutinel, Marion; Fick, Jerker; Larsson, D. G. Joakim; Flach, Carl-Fredrik

doi:10.1016/j.envpol.2021.116733

Cited by 34 publications

(23 citation statements)

References 42 publications

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“…This is the first study reporting the potentially concerning prevalence of PromA plasmids in WWTP biosolids, because PromA plasmids possess mobilization and self‐transfer capabilities potentiating transfer of resistance carrying plasmids (Werner et al ., 2020 ). Presence of IncW and IncN plasmids was reported in previous WWTPs studies (Akiyama et al ., 2010 ; Hutinel et al ., 2021 ). IncW plasmids have been isolated from different species of Enterobacteriaceae together with IncN plasmids and are of medical significance because many can confer resistance to clinical antibiotics such as carbapenems and third‐generation cephalosporins (Fernández‐López et al ., 2006 ; Eikmeyer et al ., 2012 ).…”

Section: Discussionmentioning

confidence: 99%

Biosolids for safe land application: does wastewater treatment plant size matters when considering antibiotics, pollutants, microbiome, mobile genetic elements and associated resistance genes?

Wolters

Hauschild

Blau

et al. 2022

Environmental Microbiology

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Soil fertilization with wastewater treatment plant (WWTP) biosolids is associated with the introduction of resistance genes (RGs), mobile genetic elements (MGEs) and potentially selective pollutants (antibiotics, heavy metals, disinfectants) into soil. Not much data are available on the parallel analysis of biosolid pollutant contents, RG/MGE abundances and microbial community composition. In the present study, DNA extracted from biosolids taken at 12 WWTPs (two large-scale, six middle-scale and four smallscale plants) was used to determine the abundance of RGs and MGEs via quantitative real-time PCR and the bacterial and archaeal community composition was assessed by 16S rRNA gene amplicon sequencing. Concentrations of heavy metals, antibiotics, the biocides triclosan, triclocarban and quaternary ammonium compounds (QACs) were measured. Strong and significant correlations were revealed between several target genes and concentrations of Cu, Zn, triclosan, several antibiotics and QACs. Interestingly, the size of the sewage treatment plant (inhabitant equivalents) was negatively correlated with antibiotic concentrations, RGs and MGEs abundances and had little influence on the load of metals and QACs or the microbial community composition. Biosolids from WWTPs with anaerobic treatment and hospitals in their catchment area were associated with a higher abundance of potential opportunistic pathogens and higher concentrations of QACs.

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Section: Discussionmentioning

confidence: 99%

Biosolids for safe land application: does wastewater treatment plant size matters when considering antibiotics, pollutants, microbiome, mobile genetic elements and associated resistance genes?

Wolters

Hauschild

Blau

et al. 2022

Environmental Microbiology

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show abstract

“…Multidrug-resistant P. aeruginosa carrying a carbapenemase and other mobile antibiotic resistance genes, such as bla SHV-5 , aac(3)-Ia, aac (3)-IIe , crpP , dfrA1 and qacEdelta1 , was isolated from a hot spot healthcare wastewater environment. This could be an indication of the selective advantage for the emergence of this high-level MDR uncommon ST in healthcare-associated wastewater [ 57 ].…”

Section: Discussionmentioning

confidence: 99%

Worldwide distribution and environmental origin of the Adelaide imipenemase (AIM-1), a potent carbapenemase in Pseudomonas aeruginosa

et al. 2021

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Carbapenems are potent broad-spectrum β-lactam antibiotics reserved for the treatment of serious infections caused by multidrug-resistant bacteria such as Pseudomonas aeruginosa . The surge in P. aeruginosa resistant to carbapenems is an urgent threat, as very few treatment options remain. Resistance to carbapenems is predominantly due to the presence of carbapenemase enzymes. The assessment of 147 P . aeruginosa isolates revealed that 32 isolates were carbapenem non-wild-type. These isolates were screened for carbapenem resistance genes using PCR. One isolate from wastewater contained the Adelaide imipenemase gene (bla AIM-1) and was compared phenotypically with a highly carbapenem-resistant clinical isolate containing the bla AIM-1 gene. A further investigation of wastewater samples from various local healthcare and non-healthcare sources as well as river water, using probe-based qPCR, revealed the presence of the bla AIM-1 gene in all the samples analysed. The widespread occurrence of bla AIM-1 throughout Adelaide hinted at the possibility of more generally extensive spread of this gene than originally thought. A blast search revealed the presence of the bla AIM-1 gene in Asia, North America and Europe. To elucidate the identity of the organism(s) carrying the bla AIM-1 gene, shotgun metagenomic sequencing was conducted on three wastewater samples from different locations. Comparison of these nucleotide sequences with a whole-genome sequence of a P. aeruginosa isolate revealed that, unlike the genetic environment and arrangement in P. aeruginosa , the bla AIM-1 gene was not carried as part of any mobile genetic elements. A phylogenetic tree constructed with the deduced amino acid sequences of AIM-1 suggested that the potential origin of the bla AIM-1 gene in P. aeruginosa might be the non-pathogenic environmental organism, Pseudoxanthomonas mexicana .

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“…ESBL-producing E. coli populations are defined by resistance to penicillins, cephalosporins, and aztreonam that can be inhibited by beta-lactamase inhibitors like clavulanic acid [ 13 ]. These ESBLs are highly associated with mobile genetic elements capable of being passed to other bacteria via horizontal gene transfer (HGT) [ 14 ], which can be elevated by many stressors present in the WW [ 15 ]. A better understanding of the contribution of hospital WW to the presence and dissemination of ESBLs throughout the WW treatment system is necessary to determine human health risk in downstream receiving waters, as well as further assessing the potential of resistant populations to be pathogenic.…”

Section: Introductionmentioning

confidence: 99%

Similarities in Virulence and Extended Spectrum Beta-Lactamase Gene Profiles among Cefotaxime-Resistant Escherichia coli Wastewater and Clinical Isolates

et al. 2022

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The World Health Organization has identified antibiotic resistance as one of the largest threats to human health and food security. In this study, we compared antibiotic resistance patterns between ESBL-producing Escherichia coli from human clinical diseases and cefotaxime-resistant environmental strains, as well as their potential to be pathogenic. Antibiotic susceptibility was tested amongst clinical isolates (n = 11), hospital wastewater (n = 22), and urban wastewater (n = 36, both influent and treated effluents). Multi-drug resistance predominated (>70%) among hospitalwastewater and urban wastewater influent isolates. Interestingly, isolates from clinical and urban treated effluents showed similar multi-drug resistance rates (~50%). Most hospital wastewater isolates were Phylogroup A, while clinical isolates were predominately B2, with a more diverse phylogroup population in urban wastewater. ESBL characterization of cefotaxime-resistant populations identified blaCTX-M-1 subgroup as the most common, whereby blaKPC was more associated with ceftazidime and ertapenem resistance. Whole-genome sequencing of a carbapenemase-producing hospital wastewater E. coli strain revealed plasmid-mediated blaKPC-2. Among cefotaxime-resistant populations, over 60% of clinical and 30% of treated effluent E. coli encoded three or more virulence genes exhibiting a pathogenic potential. Together, the similarity among treated effluent E. coli populations and clinical strains suggest effluents could serve as a reservoir for future multi-drug resistant E. coli clinical infections.

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Investigating the effects of municipal and hospital wastewaters on horizontal gene transfer

Cited by 34 publications

References 42 publications

Biosolids for safe land application: does wastewater treatment plant size matters when considering antibiotics, pollutants, microbiome, mobile genetic elements and associated resistance genes?

Biosolids for safe land application: does wastewater treatment plant size matters when considering antibiotics, pollutants, microbiome, mobile genetic elements and associated resistance genes?

Worldwide distribution and environmental origin of the Adelaide imipenemase (AIM-1), a potent carbapenemase in Pseudomonas aeruginosa

Similarities in Virulence and Extended Spectrum Beta-Lactamase Gene Profiles among Cefotaxime-Resistant Escherichia coli Wastewater and Clinical Isolates

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