Investigating the effects of electrical stimulation via gold nanoparticles on in vitro neurite outgrowth: Perspective to nerve regeneration

Adel, Moein; Zahmatkeshan, Masoumeh; Johari, Behrooz; Kharrazi, Sharmin; Mehdizadeh, Mehdi; Bolouri, Bahram; Rezayat, Seyed Mahdi

doi:10.1016/j.mee.2017.03.006

Cited by 27 publications

(18 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recent studies have indicated the role of several signaling pathways in the fate of ESCs, including the Activin/Nodal, Notch, BMP4, and Wnt‐signaling pathways (Rao, ; Friel et al, ; Dreesen and Brivanlou, ). Knowledge of the molecular regulatory mechanisms of pathways, which influence the gene expression of ESCs, would lead to overcoming the main challenge of maintaining the stemness of ESCs in stem cell‐based research and regenerative medicine (Yan et al, ; Adel et al, ; Lotfinia et al, ).…”

Section: Introductionmentioning

confidence: 99%

Inhibition of transcription factor T‐cell factor 3 (TCF3) using the oligodeoxynucleotide strategy increases embryonic stem cell stemness: possible application in regenerative medicine

Johari

Asadi

Rismani

et al. 2019

Cell Biology International

Self Cite

View full text Add to dashboard Cite

The transcription factor T‐cell factor 3 (TCF3), one component of the Wnt pathway, is known as a cell‐intrinsic inhibitor of many pluripotency genes in embryonic stem cells (ESCs) that influences the balance between pluripotency and differentiation. In this study, the effects of inhibition of TCF3 transcription factor on the stemness of mouse ESCs (mESCs) were investigated using the decoy oligodeoxynucleotides (ODNs) strategy. The TCF3 decoy and its scramble ODNs were designed and synthesized. The interaction specificity of the TCF3 decoy with the TCF3 transcription factor was evaluated by the electrophoretic mobility shift assay. Subcellular localization was carried out using fluorescence and confocal microscopy. Self‐renewal and pluripotency of mESCs were analyzed by 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyl tetrazolium bromide (MTT), cell cycle and apoptosis, alkaline phosphatase (ALP), embryoid body (EB) formation, and real‐time assays. All experiments were performed in triplicate. The results showed that knockdown of TCF3 by decoy ODNs transfection in mESCs led to an increase in the cell proliferation, ALP enzyme activity, and master regulatory stemness genes and a decrease in the number and diameter of EBs. These results supported TCF3 as a potential target to maintain the pluripotency and self‐renewal capacity of mESCs. Knockdown of the TCF3 transcription factor using decoy ODNs can be a promising method to maintain the stemness of stem cells in regenerative medicine and cell therapy researches.

show abstract

Section: Introductionmentioning

confidence: 99%

Inhibition of transcription factor T‐cell factor 3 (TCF3) using the oligodeoxynucleotide strategy increases embryonic stem cell stemness: possible application in regenerative medicine

Johari

Asadi

Rismani

et al. 2019

Cell Biology International

Self Cite

View full text Add to dashboard Cite

show abstract

“…It was speculated that the cells with the high density might secrete more extracellular matrix (ECM) for inducing neurotransmitter release, which led to the orientation of cells and accelerated intercellular communications through the magnetic effect. 44,45 Next, the physical characteristics of cells based on PPy substrate 2.3 aer 20 min magnetic stimulation were studied with different magnetic intensities (0 mT, 5-6 mT, 9-10 mT, and 15-16 mT). Fig.…”

Section: Magnetic Effect Of Ppy Substrate On Cellsmentioning

confidence: 99%

Influence of magnetic field on morphological structures and physiological characteristics of bEnd.3 cells cultured on polypyrrole substrates

Yang

et al. 2019

RSC Adv.

View full text Add to dashboard Cite

show abstract

“…Recently, gold nanoparticles (GNPs) were used as a new electrical conductive material to deliver electrical stimulation to NSCs. Cells cultured on GNP‐coated substrates showed higher cell viability and neurite growth with daily pulsed electrical stimulation (100 mV mm −1 , 55 min every day) …”

Section: Functions Of Matrix Materials In Neural Differentiation Frommentioning

confidence: 99%

Materials for Neural Differentiation, Trans‐Differentiation, and Modeling of Neurological Disease

et al. 2018

View full text Add to dashboard Cite

Neuron regeneration from pluripotent stem cells (PSCs) differentiation or somatic cells trans-differentiation is a promising approach for cell replacement in neurodegenerative diseases and provides a powerful tool for investigating neural development, modeling neurological diseases, and uncovering the mechanisms that underlie diseases. Advancing the materials that are applied in neural differentiation and trans-differentiation promotes the safety, efficiency, and efficacy of neuron regeneration. In the neural differentiation process, matrix materials, either natural or synthetic, not only provide a structural and biochemical support for the monolayer or three-dimensional (3D) cultured cells but also assist in cell adhesion and cell-to-cell communication. They play important roles in directing the differentiation of PSCs into neural cells and modeling neurological diseases. For the trans-differentiation of neural cells, several materials have been used to make the conversion feasible for future therapy. Here, the most current applications of materials for neural differentiation for PSCs, neuronal trans-differentiation, and neurological disease modeling is summarized and discussed.

show abstract

Investigating the effects of electrical stimulation via gold nanoparticles on in vitro neurite outgrowth: Perspective to nerve regeneration

Cited by 27 publications

References 35 publications

Inhibition of transcription factor T‐cell factor 3 (TCF3) using the oligodeoxynucleotide strategy increases embryonic stem cell stemness: possible application in regenerative medicine

Inhibition of transcription factor T‐cell factor 3 (TCF3) using the oligodeoxynucleotide strategy increases embryonic stem cell stemness: possible application in regenerative medicine

Influence of magnetic field on morphological structures and physiological characteristics of bEnd.3 cells cultured on polypyrrole substrates

Materials for Neural Differentiation, Trans‐Differentiation, and Modeling of Neurological Disease

Contact Info

Product

Resources

About