Investigating the Effect of Static Magnetic Field and Magnetic Iron Oxide Nanoparticle on Enzymatic Antioxidant Defense in Dracocephalum polychaetum Cell Suspension Culture

Abstract

This study was conducted to investigate the effect of magnetic iron oxide nanoparticles (MNP) and static magnetic field (SMF) on the activity of antioxidant enzymes in the cell suspension culture of Dracocephalum polychaetum (Lamiaceae family). The treatment procedure was done by cultivating the cells either with 100 ppm MNP, SMFs, or simultaneous exposure to both MNP and SMFs. The SMF at 30 mT was uniformly applied to the cells either for 3 or 4 days with 3 hours per day or 5 hours per day intervals, respectively. The highest activity of polyphenol oxidase (PPO), phenylalanine ammonia-lyase (PAL), catalase (CAT), malondialdehyde (MDA) content, and electrical conductivity (EC) were observed under the elicitation of the cells with simultaneous exposure to both MNP and SMFs, but the highest amount of FRAP value was observed under the elicitation of the sample with the MNP treatment. Also, the results of this study showed that the greatest activity of peroxidase (POX) was observed under SMF and MNP treatments. In general, SMF and MNP treatments caused various changes in cell structure and metabolism by inducing oxidative stress and having a direct effect on the membrane. The cell activated its enzymatic antioxidant defense system in response to these treatments, which caused changes in its activity and amount compared to the control cell.