Investigating the Effect of Experimental Hypothyroidism on Insulin/Thyroid Hormones and Hepatic Steatosis

Nazemi, Vahideh Sadat; Dayer, Mohammad Reza; Mohammadi, Tayebeh

doi:10.5812/zjrms.65536

Cited by 1 publication

(2 citation statements)

References 25 publications

(23 reference statements)

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“…Відомо, що за умов ЦД формується дисфункція гіпоталамо-гіпофізарно-тиреоїдної осі, зменшується секреція тиреотропного гормону (ТТГ) у відповідь на стимуляцію аденогіпофіза тиреоліберином гіпоталамуса. Клінічно підтверджено, що гіпотиреоз асоціюється зі зниженою чутливістю тканин до інсуліну [6][7][8][9].…”

Section: вступunclassified

“…Просвітлення цитоплазми ймовірно зумовлено втратою печінковими клітинами глікогену. Важливо, що ступінь ферментної активності може не відповідати інтенсивності структурних змін, що збігається з літературними даними і може характеризувати ранні зміни -предиктори цитолізу гепатоцитів [9,26,27].…”

Section: результати та їх обговоренняunclassified

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Predictors of Hepatocytes Cytolysis of Insulin Resistant Rats Under Conditions of Pre- And Postnatal Iodine Deficiency

Stetseviat¹,

Voronych-Semchenko²,

Bahrii³

et al. 2023

Fiziol Zh

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Carbohydrate metabolism markers, thyroid profile indexes, transaminase activity and structural features of liver were studied, taking into account sexual dimorphism, in rats which were keeping on a high-fructose diet under conditions of adequate iodine supply and pre- and postnatal iodine deficiency. It was found that under fructose loading of animals with iodine deficiency the risk of insulin resistance (IR) development increases (HOMA-IR index in insulin-resistant male rats under conditions of pre- and postnatal iodine deficiency increases respectively by 22 and 13% compared to the data in animals that received a high-carbohydrate diet and adequate iodine supply). It is important, that in males and females with IR, the secretion of free triiodothyronine decreased by 36 and 40%, thyroid-stimulating hormone – by 28 and 34%, respectively, compared to the data of intact animals. The development of IR was accompanied by the activation of aminotransferases in blood serum (more in males). In particular, in male rats with IR under conditions of pre- and postnatal iodine deficiency, the activity of aspartate aminotransferase increased by 2.7 and 2.4 times, alanine aminotransferase – by 3.9 and 2.7 times, respectively, which led to a decrease of the de-Ritis index (more significantly against the background of prenatal iodine deficiency – by 25%) in relation to the data in animals that were on a standard diet. The activity of aminotransferases in rats with IR under conditions of iodine deprivation was higher than in animals with isolated iodine deficiency – by 11-56% and IR – by 13-47 %. In animals with IR, the presence of binucleated hepatocytes with a diffuse decrease in the number of glycogen granules in them and accumulation of glycoproteins in the wall of liver vessels were found. The foci of fatty dystrophy and pronounced zonal character of dystrophic processes in hepatocytes were identified in females, diffuse dystrophy prevailed in males. In the cytoplasm of hepatocytes of animals with IR under conditions of prenatal iodine deficiency, the lightening and the granularity, single granules of glycogen were observed. Under these conditions, the average perimeter (by 74%) and area (by three times) of hepatocytes, the average area of nucleus (by 3.1 times), the average perimeter (by 50%) and area (by 66%) of the nucleolus were increased compared to the control values. The structural changes were consistent with an increase of the transaminase activity. Thus, fructose loading and the development of IR acts as a trigger for cytolysis of hepatocytes, which increases under conditions of iodine deficiency, especially prenatal.

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Section: вступunclassified

Section: результати та їх обговоренняunclassified