Investigating the cell and developmental biology of plant infection by the rice blast fungus Magnaporthe oryzae

Eseola, Alice Bisola; Ryder, Lauren S.; Osés-Ruiz, Míriam; Findlay, Kim; Xia, Yan; Cruz-Mireles, Neftaly; Molinari, Camilla; Garduño-Rosales, Marisela; Talbot, Nicholas J.

doi:10.1016/j.fgb.2021.103562

Cited by 58 publications

(65 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The ability of the rice blast fungus to colonise plant tissue and cause disease is still relatively poorly understood (Fernandez and Orth, 2018; Eseola et al, 2021). The most significant recent advances have come from exploring the cellular changes that accompany fungal infection (Khang et al, 2010; Eseola et al, 2021), the regulation of primary metabolism associated with biotrophic growth (Sun et al, 2018), the secondary metabolic pathways associated with suppression of host immunity (Patkar et al, 2015; Marroquin-Guzman et al, 2017), the definition of effector functions (Mentlak et al, 2012; Kim et al, 2020), and the identification of signalling pathways associated with invasive growth (Sakulkoo et al, 2018). These have provided insight into the substantial changes elicited by M. oryzae as it infects rice plants, in order to cause disease (Fernandez et al, 2014; Cruz-Mireles et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

The transcriptional landscape of plant infection by the rice blast fungus Magnaporthe oryzae reveals distinct families of temporally co-regulated and structurally conserved effectors

Xia

Tang

Ryder

et al. 2022

Preprint

Self Cite

View full text Add to dashboard Cite

The rice blast fungus Magnaporthe oryzae causes a devastating disease which threatens global rice production. In spite of intense study, the biology of plant tissue invasion during blast disease remains poorly understood. Here we report a high resolution, transcriptional profiling study of the entire plant-associated development of the blast fungus. Our analysis revealed major temporal changes in fungal gene expression during plant infection. Pathogen gene expression could be classified into 10 modules of temporally co-expressed genes, providing evidence of induction of pronounced shifts in primary and secondary metabolism, cell signalling and transcriptional regulation. A set of 863 genes encoding secreted proteins are differentially expressed at specific stages of infection, and 546 were predicted to be effectors and named MEP (Magnaportheeffector protein) genes. Computational prediction of structurally-related MEPs, including the MAX effector family, revealed their temporal co-regulation in the same co- expression modules. We functionally characterised 32 MEP genes and demonstrate that Mep effectors are predominantly targeted to the cytoplasm of rice cells via the biotrophic interfacial complex (BIC), and use a common unconventional secretory pathway. Taken together, our study reveals major changes in gene expression associated with blast disease and identifies a diverse repertoire of effectors critical to successful infection.

show abstract

Section: Discussionmentioning

confidence: 99%

The transcriptional landscape of plant infection by the rice blast fungus Magnaporthe oryzae reveals distinct families of temporally co-regulated and structurally conserved effectors

Xia

Tang

Ryder

et al. 2022

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…Apoptosis-like and heterokaryon incompatibility–driven cell death are not the only forms of RCD deployed by fungi. Magnaporthe oryzae relies on developmental cell death of germinating conidia during appressorium formation in rice blast disease [ 51 , 52 ]. This developmental death of fungal cells shares with animal cells hallmarks of autophagy and ferroptosis ( Fig 1D ).…”

Section: Is Rcd Involved In Innate Immunity Of Fungi?mentioning

confidence: 99%

Innate immunity in fungi: Is regulated cell death involved?

Gaspar

Pawlowska

2022

PLoS Pathog

View full text Add to dashboard Cite

“…In this study, we set out to investigate transcriptional regulation of effector-encoding genes in M. oryzae and how gene expression is orchestrated during plant infection. On the leaf surface, M. oryzae conidia germinate and sense plant surface cues which trigger development of a specialised infection cell, the appressorium, required for penetration of host cells (9)(10)(11).…”

Section: Introductionmentioning

confidence: 99%

Rgs1 is a regulator of effector gene expression during plant infection by the rice blast fungus Magnaporthe oryzae

Tang¹,

Xia²,

Ryder³

et al. 2022

Preprint

Self Cite

View full text Add to dashboard Cite

To cause rice blast disease the filamentous fungus Magnaporthe oryzae secretes a battery of effector proteins into host plant tissue to facilitate infection. Effector-encoding genes are expressed only during plant infection and show very low expression during other developmental stages. How effector gene expression is regulated in such a precise manner during invasive growth by M. oryzae is not known. Here, we report a forward-genetic screen to identify regulators of effector gene expression, based on the selection of mutants that show constitutive effector gene expression. Using this simple screen, we identify Rgs1, a regulator of G-protein signalling (RGS) protein that is necessary for appressorium development, as a novel transcriptional regulator of effector gene expression, which acts prior to plant infection. We show that an N-terminal domain of Rgs1, possessing transactivation activity, is required for effector gene regulation and acts in an RGS-independent manner. Rgs1 controls expression of at least 60 temporally co-regulated effector genes, preventing their transcription during the pre-penetration stage of development prior to plant infection. A regulator of appressorium morphogenesis is therefore also required for orchestration of pathogen gene expression required for invasive growth by M. oryzae during plant infection.

show abstract

Investigating the cell and developmental biology of plant infection by the rice blast fungus Magnaporthe oryzae

Cited by 58 publications

References 60 publications

The transcriptional landscape of plant infection by the rice blast fungus Magnaporthe oryzae reveals distinct families of temporally co-regulated and structurally conserved effectors

The transcriptional landscape of plant infection by the rice blast fungus Magnaporthe oryzae reveals distinct families of temporally co-regulated and structurally conserved effectors

Innate immunity in fungi: Is regulated cell death involved?

Rgs1 is a regulator of effector gene expression during plant infection by the rice blast fungus Magnaporthe oryzae

Contact Info

Product

Resources

About