Investigating the applicability of polar fabric phase sorptive extraction for the HPLC quantitation of salivary vitamin B12 following administration of sublingual tablets and oral sprays

Korpeti, Anastasia; Manousi, Natalia; Kabir, Abuzar; Furton, Kenneth G.; Tzanavaras, Paraskevas D.; Zacharis, Constantinos K.

doi:10.1016/j.talanta.2023.124482

Cited by 4 publications

(1 citation statement)

References 24 publications

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“…In both ways, it is possible to selectively obtain the analyte within the detectable limit. Some recent studies present the application of this unique technique to analyze favipiravir in human plasma and breast milk 10,11 pioglitazone, repaglinide, and nateglinide in human plasma, 12 vitamin B12 in saliva, 13 nonsteroidal anti-inflammatory drugs in total blood, 10 venlafaxine, paroxetine, fluoxetine, amitriptyline and clomipramine in human plasma, 14 and benzylpenicillin, cloxacillin, dicloxacillin, and oxacillin in human plasma samples. 15 As a result of the globalization of the pharmaceutical market and the establishment of consensus among pharmaceutical companies, the importance of drug safety issues has increased significantly.…”

Section: Introductionmentioning

confidence: 99%

Adapting Fabric Phase Sorptive Extraction as an Innovative Multitool for Sample Transfer and Extraction in Pharmacokinetic Analysis Followed by LC-MS Determination of Levofloxacin in Plasma Samples

Ekin Dolaksız,

Kaynak,

Kabir

et al. 2024

ACS Omega

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Fabric phase sorptive extraction (FPSE) is a simple microextraction technique that allows analytes to be rescued from matrix components while using a small volume of samples to analyze complex biological systems. This study used FPSE as a microextraction tool and a sample storage and transfer device. Levofloxacin as a model molecule was applied intravenously (IV) to New Zealand male rabbits. The samples were simultaneously extracted by using FPSE and protein precipitation methods. The final solutions were analyzed using LC-MS equipped with an ACE C18 LC Column (150 mm × 4.6 mm, 5 μm) at 25 °C employed in isocratic elution mode using solution A (0.1% formic acid in water)/solution B (0.1% formic acid in acetonitrile) (80:20, v/v). The total analysis time was less than 15 min. The developed method was validated using the ICH M10 bioanalytical method validation and study sample analysis guidelines. The results obtained using FPSE were statistically identical to those obtained using protein precipitation. The plasma samples applied onto FPSE (10 μL onto 1.0 cm × 1.0 cm Biofluid Sampler) were stored in three different temperatures [refrigerator (2−8 °C), at ambient temperature (20 ± 5 °C), and in the stability cabinet (40 °C, 75% humidity)] and three different storage conditions (Eppendorf tubes, plastic containers, and straw paper envelopes). Levofloxacin in plasma samples adsorbed by FPSE biofluid sampler remained stable at 2−8 °C in Eppendorf tubes for at least 1 week. This study showed that FPSE could be used as a sample storage and transfer device for pharmacokinetic applications that need to work with small sample volumes and discard aggressive cold chains to store and transfer the plasma samples.

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