“…Carapace replacement is a biological process in all crustacean species periodically in which the old exoskeleton (exuvium) is released and the new one is formed immediately so that in this process there is an increase in growth or weight [9], [12]- [15]. After the change of carapace, the exoskeleton (exuvium) of crabs becomes smooth or soft due to its soft (spongy) muscles with a thin membrane and accounts for 30-38% of the body weight of freshly molted soft carapace crabs, so it appears that more than 60% of the crab's body is meat [16]- [18].…”
Crustacean carapace has various functions which can be seen from the composition of the biomaterial in it. Various concentrations of inorganic biomaterial elements were investigated from the hard carapace and the newly molted (soft-shelled) (Scylla paramamosain) with SEM-EDXRS (scanning electron microscopy-Energy Dispersive X-ray Spectrometer) technique. This study traced the composition of the inorganic elements of the premolt, postmolt, intermolt and soft (exuvium) crab hard carapace tissue of mangrove crabs from the point of view. Various stages of development. Important elements such as C, O2, Mg, P, Ca, S, Na, Si, Cl, and others, are reabsorbed from the carapace into the body tissues to fulfill further needs in soft-shelled crabs and are reused to some extent during formation new carapace. This study provides evidence that, inorganic elements in freshly molted soft carapace crabs are less common than hard carapace crabs
“…Carapace replacement is a biological process in all crustacean species periodically in which the old exoskeleton (exuvium) is released and the new one is formed immediately so that in this process there is an increase in growth or weight [9], [12]- [15]. After the change of carapace, the exoskeleton (exuvium) of crabs becomes smooth or soft due to its soft (spongy) muscles with a thin membrane and accounts for 30-38% of the body weight of freshly molted soft carapace crabs, so it appears that more than 60% of the crab's body is meat [16]- [18].…”
Crustacean carapace has various functions which can be seen from the composition of the biomaterial in it. Various concentrations of inorganic biomaterial elements were investigated from the hard carapace and the newly molted (soft-shelled) (Scylla paramamosain) with SEM-EDXRS (scanning electron microscopy-Energy Dispersive X-ray Spectrometer) technique. This study traced the composition of the inorganic elements of the premolt, postmolt, intermolt and soft (exuvium) crab hard carapace tissue of mangrove crabs from the point of view. Various stages of development. Important elements such as C, O2, Mg, P, Ca, S, Na, Si, Cl, and others, are reabsorbed from the carapace into the body tissues to fulfill further needs in soft-shelled crabs and are reused to some extent during formation new carapace. This study provides evidence that, inorganic elements in freshly molted soft carapace crabs are less common than hard carapace crabs
“…Prevalence among summer active wild crabs from Delaware Bay and Albemarle Sound was 20.0 and 14.7%, respectively (Table 1). CsRV1 prevalence among active crabs from Chesapeake Bay was based on previous studies (Flowers et al 2016b, Spitznagel et al 2019, which found an average of 21.2% for Upper Chesapeake Bay and 12.5% for Lower Chesapeake Bay (Fig. 3).…”
Section: Csrv1 Prevalence In Overwintering C Sapidusmentioning
confidence: 93%
“…Approximately 50 mg of muscle and epidermis tissue were dissected from a walking leg and homogenized using a Savant MP ® FastPrep24 homogenizer with ceramic beads in 1.0 ml TRIzol (VWR Scientific) or homemade Trizol substitute (Rodríguez-Ezpeleta et al 2009). RNA extraction followed the protocol used by Spitznagel et al (2019). RNA pellets were dis- Table 1 for location names solved in 50 μl 1 mM EDTA and stored at −80°C.…”
Section: Dissection and Rna Extractionmentioning
confidence: 99%
“…Viral dsRNA was purified using CF11 cellulose affinity chromatography (Flowers et al 2016b) from crabs heavily infected with CsRV1 (>10 8 co pies mg −1 muscle), and then quantified and serially diluted in 25 ng μl −1 yeast tRNA carrier. The qPCR cycling conditions and reagents were as described by Spitznagel et al (2019), using qScript™ One-Step qRT-PCR Kit (Quanta Bio) in 10 μl reactions containing 0.5 μM of each primer. To anneal PCR primers to dsRNA, primers and extracted RNA were combined, heated to 95°C for 5 min then cooled to 4°C prior to being added to the reverse transcriptase and Taq polymerase reaction mixture.…”
Section: Quantitative Reverse Transcription Pcr Of Csrv1mentioning
confidence: 99%
“…Various viruses have been described, with the most studied being Callinectes sapidus reovirus 1 (CsRV1). It was identified as a cause of mortality in captive blue crabs, particularly in soft crab aquaculture (Johnson 1977, Bowers et al 2010, Spitznagel et al 2019, and in the laboratory, individuals experimentally infected with CsRV1 suffered 100% mortality within 16 d (Bowers et al 2010). The virus infects hemocytes, gills, nervous system, and connective tissue, which in turn invades the brain and thoracic globules and is associated with tremors and paralysis (Johnson 1977).…”
Among the many Callinectes spp. across the western Atlantic, the blue crab C. sapidus has the broadest latitudinal distribution, encompassing both tropical and temperate climates. Its life history varies latitudinally, from extended overwintering at high latitudes to year-round activity in tropical locations. Callinectes sapidus reovirus 1 (CsRV1) is a pathogenic virus first described in North Atlantic C. sapidus and has recently been detected in southern Brazil. Little information exists about CsRV1 prevalence at intervening latitudes or in overwintering blue crabs. Using a quantitative reverse transcription PCR (RT-qPCR) method, this study investigated CsRV1 prevalence in C. sapidus across latitudinal differences in temperature and crab life history, as well as in additional Callinectes spp. and within overwintering C. sapidus. CsRV1 prevalence in C. sapidus was significantly correlated with high water temperature and blue crab winter dormancy. Prevalence of CsRV1 in C. sapidus on the mid-Atlantic coast was significantly lower in winter than in summer. CsRV1 infections were not detected in other Callinectes spp. These findings revealed that CsRV1 is present in C. sapidus across their range, but not in other Callinectes species, with prevalence associated with temperature and host life history. Such information helps us to better understand the underlying mechanisms that drive marine virus dynamics under changing environmental conditions.
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