Investigating retroviral envelope proteome plasticity

Progress on the labeling and single-particle tracking technologies of viruses

“…[13][14][15][16][17][18][19][20][21][22] Now, it is feasible to respectively fuse several FPs with different colours to different viral proteins in the same virion, [18][19][20][21] which is useful in tracking the infection and assembly processes. Besides, viruses can be labeled with FPs by decorating modied green uorescence protein (GFP) forms to their surfaces, which is realized by fusing GFP to either glycosylphosphatidylinositol (GPI)anchor acceptor sequences [23][24][25][26] or viral membrane targeting sequences. 13,16,19 FPs can also be used to label viral nucleic acid if it includes sequence motifs specically recognized by RNAbinding proteins.…”