Investigating proteomic methods and tools to assess sperm quality

Sergeant, Nicolas; Jumeau, Fanny; Eddarkaoui, Sabiha; Sigala, Julien; Dossou, Gbete Florian; Delehedde, Maryse; Buée, Luc; Yvoz, Jean François; Mitchell, Valerie A.

doi:10.1016/j.anireprosci.2016.03.072

Cited by 4 publications

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“…The proAKAP4 dosage sperm quality and functionality parameter in the dromedary camel may reflect the best fertility period. Low levels of AKAP4, and/or that of the proAKAP4 precursor molecule, were previously described as a pertinent sperm parameter for sperm quality assessments and to predict fertility in pig and bulls ( Peddinti et al 2008 , Sergeant et al 2016 , 2019 , Delehedde et al 2018 , Ruelle et al 2019 ). ProAKAP4 concentrations have been correlated with fertility in pigs ( Sergeant et al 2019 ), bulls ( Peddinti et al 2008 , Singh et al 2019 ) and mice ( Miki et al 2002, Fang et al 2019 , Xu et al 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Identification of proAKAP4 concentration variations in dromedary sperm and their correlation with monthly semen parameters

Malo¹,

Carracedo²,

Delehedde³

et al. 2021

Reproduction and Fertility

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ProAKAP4 is synthetized as a precursor polypeptide that must be converted into mature AKAP4 in living spermatozoa and is considered as a functional marker of spermatozoa. The gene is well-conserved in mammals although uncharacterized in Camelidae. In the present study, we investigate the expression metabolism of proAKAP4 and AKAP4 proteins and evaluate their seasonal dynamics relative to semen quality in dromedary camels. Semen parameters including volume and viscosity, and characteristics of sperm including concentration, total production, total and progressive motility, vitality, acrosome integrity and morphological abnormalities were assessed in semen samples collected weekly from 6 camels during the rutting season, from November to April. Only total sperm production varied, peaking in January. Both the precursor ProAKAP4 and AKAP4 proteins were investigated and shown to express biochemical properties similar to those described in other mammals. ProAKAP4 concentrations expressed in ng / 10 million spermatozoa as assayed using a specific ELISA showed a strong positive correlation with ejaculate volume (p=0.045), viscosity (p<0.001) and sperm total motility (p=0.049). Furthermore, their concentrations exhibited clear seasonal variations in camel semen. In conclusion, the assessment of proAKAP4 concentrations in camel sperm provide a novel parameter to assess sperm quality. Further studies should be performed to investigate proAKAP4 concentrations relative to fertility in Camelidae that may help to define the right time for mating and semen collection and increase the success of breeding programmes.

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Section: Discussionmentioning

confidence: 99%

Identification of proAKAP4 concentration variations in dromedary sperm and their correlation with monthly semen parameters

Malo¹,

Carracedo²,

Delehedde³

et al. 2021

Reproduction and Fertility

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“…Indeed, proAKAP4 is one of fertility associated protein markers that have been identified more than 30 years from now and have been today developed as a robust tool as we will described below. With the 4MID® kits, that quantify the proAKAP4 is fresh as in frozen semen, breeders have today a valuable tool to evaluate semen quality and predict fertility in bull and swine [11,12]. This newly approach to evaluate semen quality and fertility is based on a protein marker detected through a sandwich ELISA kit format that analyze up to 87 semen simultaneously (Table 1).…”

Section: Journal Of Dairy and Veterinary Sciencesmentioning

confidence: 99%

“…ing ELISA approaches, only request a plate reader equipment. The concentrations of proAKAP4 using the Pig 4MID® kit as measured in semen of 13 boars with similar sperm parameters, exhibited striking variations from a boar to another stressing out the interest to have a functional sperm parameter [11,12]. When the same semen doses of those followed animals were used to inseminate 210 sows, an increase of both fertility index and prolificity were observed in the groups of swine gathered according a threshold concentration of 45ng of proAKAP4 /10 millions of spermatozoa (unpublished data).…”

Section: Journal Of Dairy and Veterinary Sciencesmentioning

confidence: 99%

The Sperm Specific Protein Proakap4 as an Innovative Marker to Evaluate Sperm Quality and Fertility

Delehedde

2019

JDVS

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“…Unravelling the events that govern a conception success or failure, and understanding the molecular cascades that regulate fertility, requires a systems biology approach utilizing high throughput omics technologies. The advent of increasingly sophisticated proteomic technologies has enabled many detailed investigations, not only into the composition of mammalian spermatozoa (Baker et al 2008, Somashekar et al 2015, Swegen et al 2015, Sergeant et al 2016, Martín-Cano et al 2020, but also into the identification of numerous proteomic fertility biomarker candidates in mice (Baker et al 2005), men (de Mateo et al 2007, Netherton et al 2017, bulls (Park et al 2012, Somashekar et al 2017 and boars (Kwon et al 2015a,b). To our knowledge, only one study has utilized omics technologies in identifying such biomarkers in stallion ejaculates (Novak et al 2010), and reported that sperm protein expression accounted for only 10% of fertility variability.…”

Section: Introductionmentioning

confidence: 99%

Mass spectrometry reveals distinct proteomic profiles in high- and low-quality stallion spermatozoa

et al. 2020

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The horse breeding industry relies upon optimal stallion fertility. Conventional sperm assessments provide limited information regarding ejaculate quality and are not individually predictive of fertilising potential. The aim of this study was to harness mass spectrometry to compare the proteomic profiles of high- and low-quality stallion spermatozoa, with the ultimate goal of identifying fertility biomarker candidates. Extended stallion semen (n=12) was fractionated using Percoll density gradients to isolate low-quality and high-quality sperm populations. Motility and morphological assessments were carried out, and proteomic analyses was conducted using UHPLC-MS/MS. High-quality spermatozoa recorded higher total (95.2±0.52% vs 70.6±4.20%; P≤0.001) and progressive motilities (43.4±3.42% vs 27.3±4.32%; P≤0.05), and a higher proportion of morphologically normal cells (50.2±4.34% vs 38.8±2.72%; P≤0.05). 1,069 proteins were quantified by UHPLC-MS/MS, of which 22 proteins were significantly more abundant in the high-quality sperm population (P≤0.05). A-kinase anchor protein 4 (AKAP4) and Hexokinase 1 (HK1) were considered possible biomarker candidates and their differential expression was confirmed by immunoblot. Protein expression was significantly correlated with total (AKAP4 R2=0.38, P≤0.01; HK1 R2=0.46, P≤0.001) and progressive motilities (AKAP4 R2=0.51, P≤0.001; HK1 R2=0.55, P≤0.01), percentage rapid (AKAP4 R2=0.29, P≤0.05; HK1 R2=0.58, P≤0.001), straight line velocity (HK1 R2=0.50, P≤0.01) and straightness (HK1 R2=0.40, P≤0.01). Furthermore, AKAP4 was highly susceptible to adduction by 4-hydroxynonenal (4HNE), which resulted in a global reduction in the phosphorylation profiles following capacitation. In conclusion, the proteomic profiles of high- and low-quality stallion spermatozoa differ substantially, and proteins such as AKAP4 and HK1 could serve as biomarkers of ejaculate quality.

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Investigating proteomic methods and tools to assess sperm quality

Cited by 4 publications

References 0 publications

Identification of proAKAP4 concentration variations in dromedary sperm and their correlation with monthly semen parameters

Identification of proAKAP4 concentration variations in dromedary sperm and their correlation with monthly semen parameters

The Sperm Specific Protein Proakap4 as an Innovative Marker to Evaluate Sperm Quality and Fertility

Mass spectrometry reveals distinct proteomic profiles in high- and low-quality stallion spermatozoa

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