Investigating Protein Interactions at Solid Surfaces—In Situ, Nonlabeling Techniques

Svensson, Olof; Sotres, Javier; Barrantes, Alejandro

doi:10.1002/9781118523063.ch6

Cited by 1 publication

(3 citation statements)

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“…The polymer stock solution (0.5 mL) was then injected, reaching the final desired concentration. The adsorption was monitored for at least 1 h or until a stable plateau value was established. , …”

Section: Methodsmentioning

confidence: 99%

“…The adsorption was monitored for at least 1 h or until a stable plateau value was established. 16,41 Examples of how the measured adsorption varies with time are provided in Figure 2. The complete set of measurements is provided in the Supporting Information.…”

Section: γ = CDmentioning

confidence: 99%

“…The adsorption was then monitored, during at least 1h, or until a stable plateau value was established. 41,42 Examples of how the measured adsorption varies with time, are provided in Figure 2 (more examples see Supporting Material). We find stable and clear plateau adsorption values, although there is some inevitable noise.…”

Section: Methodsmentioning

confidence: 99%

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Polyelectrolyte Adsorption on Solid Surfaces: Theoretical Predictions and Experimental Measurements

Xie¹,

Nylander²,

Piculell³

et al. 2013

Langmuir

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This work utilizes a combination of theory and experiments, to explore the adsorption of two different cationic polyelectrolytes onto oppositely charged silica surfaces, at pH 9.Both polymers, Poly(diallyldimethylammonium chloride), PDADMAC, and Poly(4-vinyl Nmethylpyridinium iodide), PVNP, are highly charged, and highly soluble in water. Another important aspect is that a silica surface carries a relatively high surface charge density, at this pH level. This means that we have specifically chosen to investigate adsorption under conditions where electrostatics can be expected to dominate the interactions. Of specific focus in this work is the response of the adsorption to the addition of simple salt, i.e. a process * To whom correspondence should be addressed 1 where electrostatics is gradually screened out. Theoretical predictions from a recently developed correlation-corrected classical density functional theory for polyelectrolytes, are evaluated by direct quantitative comparisons with corresponding experimental data, as obtained by ellipsometry measurements. We find that, at low concentrations of simple salt, the adsorption increases with ionic strength, reaching a maximum at intermediate levels (about 200 mM). The adsorption then drops, but retains a finite level even at very high salt concentrations, indicating the presence of non-electrostatic contributions to the adsorption. In the theoretical treatment, the strength of this relatively modest, but otherwise largely unknown, non-electrostatic surface affinity, was estimated by matching predicted and experimental slopes of adsorption curves at high ionic strength. Given these estimates for the non-electrostatic part, experimental adsorption data are essentially captured with quantitative accuracy by the classical density functional theory.

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Section: Methodsmentioning

confidence: 99%

Section: γ = CDmentioning

confidence: 99%