Investigating Possible Interspecies Communication of Plasmids Associated with Transfer of Third-Generation Cephalosporin, Quinolone, and Colistin Resistance Between Simultaneously Isolated Escherichia Coli and Klebsiella Pneumoniae

Quan, Jingjing; Hu, Huangdu; Zhang, Huichuan; Meng, Yan; Liao, Weichao; Zhou, Junxin; Han, Xinhong; Shi, Qiucheng; Zhao, Dongdong; Wang, Qian; Jiang, Yan; Yu, Yunsong

doi:10.1128/spectrum.03554-22

Cited by 4 publications

(1 citation statement)

References 40 publications

(44 reference statements)

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“…The presence of contaminants in the environment including antibiotics, quaternary ammonium compounds, and other biocides and metals (e.g., zinc and copper) can create co‐selective pressures, whereby a single agent can select for resistance to multiple agents and other antimicrobials because of the physical co‐location of the genes conferring resistance (Wales & Davies, 2015). There are many reports indicating the transfer of plasmid‐encoded genes for resistance to clinically important antibiotics such as quinolones, carbapenems and colistin, in Escherichia coli and Klebsiella pneumoniae , other Enterobacteriaceae and Pseudomonas aeruginosa isolated from raw meat, farm animals (Liu et al., 2016), inpatients (Ma et al., 2022; Martínez‐Martínez et al., 1998; Quan et al., 2023) and aquatic environments, raising serious concerns about its possible cross‐environment dissemination (Zhu et al., 2020).…”

Section: Introductionmentioning

confidence: 99%

Arctic plasmidome analysis reveals distinct relationships among associated antimicrobial resistance genes and virulence genes along anthropogenic gradients

Makowska‐Zawierucha,

Trzebny,

Zawierucha

et al. 2024

Global Change Biology

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Polar regions are relatively isolated from human activity and thus could offer insight into anthropogenic and ecological drivers of the spread of antibiotic resistance. Plasmids are of particular interest in this context given the central role that they are thought to play in the dissemination of antibiotic resistance genes (ARGs). However, plasmidomes are challenging to profile in environmental samples. The objective of this study was to compare various aspects of the plasmidome associated with glacial ice and adjacent aquatic environments across the high Arctic archipelago of Svalbard, representing a gradient of anthropogenic inputs and specific treated and untreated wastewater outflows to the sea. We accessed plasmidomes by applying enrichment cultures, plasmid isolation and shotgun Illumina sequencing of environmental samples. We examined the abundance and diversity of ARGs and other stress‐response genes that might be co/cross‐selected or co‐transported in these environments, including biocide resistance genes (BRGs), metal resistance genes (MRGs), virulence genes (VGs) and integrons. We found striking differences between glacial ice and aquatic environments in terms of the ARGs carried by plasmids. We found a strong correlation between MRGs and ARGs in plasmids in the wastewaters and fjords. Alternatively, in glacial ice, VGs and BRGs genes were dominant, suggesting that glacial ice may be a repository of pathogenic strains. Moreover, ARGs were not found within the cassettes of integrons carried by the plasmids, which is suggestive of unique adaptive features of the microbial communities to their extreme environment. This study provides insight into the role of plasmids in facilitating bacterial adaptation to Arctic ecosystems as well as in shaping corresponding resistomes. Increasing human activity, warming of Arctic regions and associated increases in the meltwater run‐off from glaciers could contribute to the release and spread of plasmid‐related genes from Svalbard to the broader pool of ARGs in the Arctic Ocean.

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Section: Introductionmentioning

confidence: 99%

Arctic plasmidome analysis reveals distinct relationships among associated antimicrobial resistance genes and virulence genes along anthropogenic gradients

Makowska‐Zawierucha,

Trzebny,

Zawierucha

et al. 2024

Global Change Biology

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Antibiotic resistance of Escherichia coli isolated from food and clinical environment in China from 2001 to 2020

Liu,

Sun,

Sun

et al. 2024

Science of The Total Environment

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Enterobacteria in anaerobic digestion of dairy cattle wastewater: Assessing virulence and resistance for one health security

Menezes,

Duarte,

Moreira

et al. 2024

Water Research

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Investigating Possible Interspecies Communication of Plasmids Associated with Transfer of Third-Generation Cephalosporin, Quinolone, and Colistin Resistance Between Simultaneously Isolated Escherichia Coli and Klebsiella Pneumoniae

Abstract: The genome sequence of bacterial pathogens commonly provides a detailed clue of genetic communication among clones or even distinct species. The intestinal microecological environment is a representative ecological niche for genetic communication.

Cited by 4 publications

References 40 publications

Arctic plasmidome analysis reveals distinct relationships among associated antimicrobial resistance genes and virulence genes along anthropogenic gradients

Arctic plasmidome analysis reveals distinct relationships among associated antimicrobial resistance genes and virulence genes along anthropogenic gradients

Antibiotic resistance of Escherichia coli isolated from food and clinical environment in China from 2001 to 2020

Enterobacteria in anaerobic digestion of dairy cattle wastewater: Assessing virulence and resistance for one health security

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