Investigating Polymorphisms and Expression Profile of Immune, Antioxidant, and Erythritol-Related Genes for Limiting Postparturient Endometritis in Holstein Cattle

Abstract

This study looked at genetic polymorphisms and transcript levels of immune, antioxidant, and erythritol-related markers for postparturient endometritis prediction and tracking in Holstein dairy cows. One hundred and thirty female dairy cows (65 endometritis affected and 65 apparently healthy) were used. Nucleotide sequence variations between healthy and endometritis-affected cows were revealed using PCR-DNA sequencing for immune (TLR4, TLR7, TNF-α, IL10, NCF4, and LITAF), antioxidant (ATOX1, GST, and OXSR1), and erythritol-related (TKT, RPIA, and AMPD1) genes. Chi-square investigation exposed a noteworthy variance amongst cow groups with and without endometritis in likelihood of dispersal of all distinguished nucleotide variants (p < 0.05). The IL10, ATOX1, and GST genes were expressed at substantially lower levels in endometritis-affected cows. Gene expression levels were considerably higher in endometritis-affected cows than in resistant ones for the genes TLR4, TLR7, TNF-α, NCF4, LITAF, OXSR1, TKT, RPIA, and AMPD1. The sort of marker and vulnerability or resistance to endometritis had a significant impact on the transcript levels of the studied indicators. The outcomes might confirm the importance of nucleotide variants along with gene expression patterns as markers of postparturient endometritis susceptibility/resistance and provide a workable control plan for Holstein dairy cows.

DNA Polymorphisms and mRNA Levels of Immune Biomarkers as Candidates for Inflammatory Postpartum Disorders Susceptibility in Italian Buffaloes

“…In order to better understand the resistance and vulnerability of inflammatory reproductive diseases in livestock, genetic polymorphisms of immunological markers for postpartum endometritis were examined in Holstein dairy cows [ 43 ]. Using PCR-DNA sequencing for the immunological ( TLR4 , IL10 , TLR7 , NCF4 , TNF-α , and LITAF ) genes, there were changes in the nucleotide sequence between endometritis-affected cows and healthy ones.…”

“…When compared to resistant cows, endometritis-affected cows had considerably higher levels of the genes TLR4 , TLR7 , TNF , NCF4 , and LITAF expression. Meanwhile, the expression of the IL10 gene was much decreased [ 43 ]. SELL , SLC11A1 , and FEZL gene expression were significantly higher in mastitic Holstein and Brown Swiss dairy cows than in tolerant animals [ 46 ].…”

New Insights into Polymorphisms in Candidate Genes Associated with Incidence of Postparturient Endometritis in Ossimi Sheep (Ovis aries)

“…In Barki sheep, the incidence of postpartum sickness was also linked to TLR4 and SOD SNPs [34]. Genetic polymorphisms of immunological, metabolic, and antioxidant indicators for postparturient endometritis were investigated in Holstein dairy cattle in order to increase an enhanced considerate of resistance and sensitivity of inflammatory reproductive disorders in cattle [35]. The nucleotide sequences of cows with endometritis and those in good health differed.…”

“…Endometritis-affected cows expressed significantly more NCF4, LITAF, TLR4, OXSR1, TLR7, TNF-α, TKT, RPIA, and AMPD1 genes than resistant cows did. In the meantime, there was a significant drop in the expression of the GST, ATOX1, and IL10 genes [35]. Dairy calves with subclinical endometritis had specific transcript levels of C2, LTF, TRAPPC13, C3, and PF4 in their blood and endometrium [41].…”

Gene polymorphism in a population of Charolais beef cattle using DNA-markers

“…It involves using both commercial breeds of animals and native aborigine ones (Curone et al, 2018;Gogoi et al, 2021). From the standpoint of general MAS strategy, noteworthy are the studies on genes, the products of which are directly associated with the regulation of the immune system of the organism and, thus, with the indices of resistance/sensitivity to different diseases (Al-Sharif et al, 2023). As for cattle genetics, in the context of issues of resistance to diseases, some of the best-investigated genes relevant for the study are the TLR-family (the family of toll-like receptors) (Mazzone et al, 2023;Maljković 2023), IFNGR2 (Muthusamy et al, 2019;Holder et al, 2020), TNFα (Sattar et al, 2019), and MBL1 (Aksel et al, 2021;Kamaldeep et al, 2021;Moretti et al, 2021).…”