Investigating new serological and tissue markers for the follow‐up of patients operated for alveolar echinococcosis

Bellanger, Anne‐Pauline; Wang, Junhua; Gbaguidi‐Haore, Houssein; Barrera, Coralie; Bresson‐Hadni, Solange; Zlobec, Inti; Lachenmayer, Anja; Richou, Carine; Turco, Célia; Gottstein, Bruno; Millon, Laurence; Beldi, Guido

doi:10.1111/pim.12827

Cited by 8 publications

(3 citation statements)

References 16 publications

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“…Moreover, the expression of programmed cell death protein ligand 1 (PD-L1) in surgical specimens might indicate the postoperative outcomes in liver AE lesions [ 30 ]. Interestingly, Bellanger and colleagues showed that surgical treatment of the liver AE patients results in a reduction of serologic PD-L1 levels [ 31 ]. Molecular methods have also become more common in the diagnosis process.…”

Section: Discussionmentioning

confidence: 99%

Basic Operative Tactics for Pulmonary Echinococcosis in the Era of Endostaplers and Energy Devices

et al. 2023

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Human echinococcosis is a zoonotic infection caused by the larvae of the tapeworm species Echinococcus. The liver is the most common location for a primary echinococcosis. However, the parasite may bypass or spread from the liver to the lungs, causing primary or secondary pulmonary echinococcosis, respectively. Pulmonary echinococcosis is a clinically challenging condition in which anthelminthic regiments are important, but surgery has the central role in removing the cysts and preventing recurrences. Surgical treatment may involve cystotomy, enucleation, capitonnage, or atypical resections, which occasionally are in combination with hepatic procedures. The utilization of modern devices is greatly underdescribed in surgery for thoracic infections, even though these facilitate much of the work. Therefore, this article aims to describe pulmonary echinococcosis and the role of modern surgical devices in the treatment process. Furthermore, we report surgical treatment of three different cases of pulmonary echinococcosis. Surgeries of uncomplicated and ruptured hepatic or pulmonary cysts are described. Simple small pulmonary echinococcal lesions can be excised by endostaplers both for diagnostic and curative reasons. Larger cysts can be removed by energy devices unless large bronchial air leaks occur. Complicated cysts require treatment by more extensive techniques. Inexperienced surgeons should not abstain but should carefully decide preoperatively how to proceed.

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Section: Discussionmentioning

confidence: 99%

Basic Operative Tactics for Pulmonary Echinococcosis in the Era of Endostaplers and Energy Devices

et al. 2023

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“…Secondly, our ELISA approach (IgG Detection ELISA) did not allow us to differentiate between present and past infections, likely leading to the detection of recovered animals that would have remained undetected in PMs. ELISA can be further developed by targeting IgM for acute infections [52] and by the detection of the IgG titer in paired sampling [53] to have a clearer picture about active and past infections. The gap between the detection rate of ELISA and PM may increase when aged animals (with a higher expected proportion of recovered animals) are sacrificed and examined for the presence of cysts.…”

Section: Discussionmentioning

confidence: 99%

IEg67 kDa Bovine Hydatid Cyst Antigen: A Candidate for Developing Sero-Diagnostic Assays for Cystic Echinococcosis, a Disease of One Health Importance

Cable

Younus

Rashid

et al. 2023

Animals

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Cystic echinococcosis (hydatidosis) is a world-wide zoonotic disease of mainly humans, livestock and dogs, caused by Echinococcus granulosus. The disease can negatively impact food production and animal welfare and causes socio-economic hardship. Here, we aimed to identify the local bovine hydatid cyst fluid (BHCF) antigen for developing a sero-diagnostic assay to be used for the pre-slaughter screening of food animals. In total, 264 bovines approved for slaughter in Pakistan were subjected to serum collection and post-mortem screening for hydatid cysts. These cysts were assessed microscopically to assess fertility and viability, and by PCR for molecular confirmation of species. A BHCF antigen was identified from positive sera via SDS-PAGE, confirmed by Western blot, and quantified via a bicinchoninic acid (BCA) assay. The quantified crude BHCF antigen (iEg67 kDa) was then used in ELISA screening to test all sera collected from known positive and negative animals based on hydatid cyst presence/absence. Of the 264 bovines examined, 38 (14.4%) showed hydatid cysts during post-mortem examination. All of these individuals, plus an additional 14 (total: 52; 19.6%) tested positive based on less time-consuming ELISA examination. Based on ELISA, occurrence in females (18.8%) was significantly higher than in males (9.2%) and was higher in cattle (19.5%) compared to buffalo (9.5%). The infection rate increased with age in both host species: cumulatively, 3.6% in animals aged 2–3 years, 14.6% in 4–5-year-olds and 25.6% in 6–7-year-olds. The occurrence of cysts in cattle was significantly higher in the lungs (14.1%) compared to their livers (5.5%), whereas the opposite was true in buffalo (6.6% livers, 2.9% lungs). For both host species, most cysts in the lungs were fertile (65%), while the majority in the liver were sterile (71.4%). We conclude that the identified iEg67 kDa antigen is a strong candidate for the development of a sero-diagnostic screening assay for the pre-slaughter diagnosis of hydatidosis.

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“…Diagnosis of AE largely depends on a combination of imaging and serological tests [5][6][7]. However, imaging studies including ultrasonography (US), computed tomography (CT), and/or magnetic resonance (MR) examinations often require a differential diagnosis from other hepatic space-occupying lesions, even for experienced physicians due to their atypical findings.…”

Section: Introductionmentioning

confidence: 99%

Echinococcus granulosus Protoscolex DM9 Protein Shows High Potential for Serodiagnosis of Alveolar Echinococcosis

2022

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Alveolar echinococcosis (AE) caused by infection with E. multilocularis metacestode, represents one of the most fatal helminthic diseases. AE is principally manifested with infiltrative, proliferating hepatic mass, resembling primary hepatocellular carcinoma. Sometimes metastatic lesions are found in nearby or remote tissue. AE diagnosis largely depends on imaging studies, but atypical findings of imaging features frequently require differential diagnosis from other hepatic lesions. Serological tests may provide further evidence, while obtaining reliable AE materials is not easy. In this study, alternative antigens, specific to AE were identified by analyzing E. granulosus protoscolex proteins. An immunoblot analysis of E. granulosus protoscolex showed that a group of low-molecular-weight proteins in the range from 14 kDa to 16 kDa exhibited a sensitive and specific immune response to AE patient sera. Partial purification and proteomic analysis indicated that this protein group contained myosin, tubulin polymerization promoting protein, fatty-acid binding protein, uncharacterized DM9, heat shock protein 90 cochaperone tebp P-23, and antigen S. When the serological applicability of recombinant forms of these proteins was assessed using enzyme-linked immunosorbent assay, DM9 protein (rEgDM9) showed 90.1% sensitivity (73/81 sera tested) and 94.5% specificity (172/181 sera tested), respectively. rEgDM9 showed weak cross-reactions with patient sera from the transitional and chronic stages of cystic echinococcosis (3 to 5 stages). rEgDM9 would serve as a useful alternative antigen for serodiagnosis of both early- and advanced-stage AE cases.

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Investigating new serological and tissue markers for the follow‐up of patients operated for alveolar echinococcosis

Cited by 8 publications

References 16 publications

Basic Operative Tactics for Pulmonary Echinococcosis in the Era of Endostaplers and Energy Devices

Basic Operative Tactics for Pulmonary Echinococcosis in the Era of Endostaplers and Energy Devices

IEg67 kDa Bovine Hydatid Cyst Antigen: A Candidate for Developing Sero-Diagnostic Assays for Cystic Echinococcosis, a Disease of One Health Importance

Echinococcus granulosus Protoscolex DM9 Protein Shows High Potential for Serodiagnosis of Alveolar Echinococcosis

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