Investigating neuronal activity by SPYCODE multi-channel data analyzer

Bologna, Luca; Pasquale, Valentina; Garofalo, Matteo; Gandolfo, Mauro; Baljon, Pieter Laurens; Maccione, Alessandro; Martinoia, Sergio; Chiappalone, Michela

doi:10.1016/j.neunet.2010.05.002

Cited by 121 publications

(96 citation statements)

References 61 publications

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“…After 1200X amplification (MEA 1060, MCS), sample the signal at 10 kHz using the MCS data acquisition card. Analyze the data (spike and burst detection) using a custom software package 23 .…”

Section: Establish the Neurophysiological Profile Of Hipsc-derived Nementioning

confidence: 99%

Rapid Neuronal Differentiation of Induced Pluripotent Stem Cells for Measuring Network Activity on Micro-electrode Arrays

Frega

Gestel

Linda

et al. 2017

JoVE

114

189

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Neurons derived from human induced Pluripotent Stem Cells (hiPSCs) provide a promising new tool for studying neurological disorders. In the past decade, many protocols for differentiating hiPSCs into neurons have been developed. However, these protocols are often slow with high variability, low reproducibility, and low efficiency. In addition, the neurons obtained with these protocols are often immature and lack adequate functional activity both at the single-cell and network levels unless the neurons are cultured for several months. Partially due to these limitations, the functional properties of hiPSC-derived neuronal networks are still not well characterized. Here, we adapt a recently published protocol that describes production of human neurons from hiPSCs by forced expression of the transcription factor neurogenin-2 12. This protocol is rapid (yielding mature neurons within 3 weeks) and efficient, with nearly 100% conversion efficiency of transduced cells (>95% of DAPI-positive cells are MAP2 positive). Furthermore, the protocol yields a homogeneous population of excitatory neurons that would allow the investigation of celltype specific contributions to neurological disorders. We modified the original protocol by generating stably transduced hiPSC cells, giving us explicit control over the total number of neurons. These cells are then used to generate hiPSC-derived neuronal networks on micro-electrode arrays. In this way, the spontaneous electrophysiological activity of hiPSC-derived neuronal networks can be measured and characterized, while retaining interexperimental consistency in terms of cell density. The presented protocol is broadly applicable, especially for mechanistic and pharmacological studies on human neuronal networks.

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Section: Establish the Neurophysiological Profile Of Hipsc-derived Nementioning

confidence: 99%

Rapid Neuronal Differentiation of Induced Pluripotent Stem Cells for Measuring Network Activity on Micro-electrode Arrays

Frega

Gestel

Linda

et al. 2017

JoVE

114

189

View full text Add to dashboard Cite

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“…To analyse the neural network activity, we recorded spontaneous bursting activity for 15 min. All of the signal analyses and statistics were performed using custom made software SPYCODE (Matlab) [28]. The detection of recorded extracellular spikes was based on the Precise Timing Spike Detection method [29].…”

mentioning

confidence: 99%

Modulation of network activity and induction of homeostatic synaptic plasticity by enzymatic removal of heparan sulfates

Korotchenko

Cingolani

Kuznetsova

et al. 2014

Phil. Trans. R. Soc. B

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Heparan sulfates (HSs) are complex and highly active molecules that are required for synaptogenesis and long-term potentiation. A deficit in HSs leads to autistic phenotype in mice. Here, we investigated the long-term effect of heparinase I, which digests highly sulfated HSs, on the spontaneous bioelectrical activity of neuronal networks in developing primary hippocampal cultures. We found that chronic heparinase treatment led to a significant reduction of the mean firing rate of neurons, particularly during the period of maximal neuronal activity. Furthermore, firing pattern in heparinasetreated cultures often appeared as epileptiform bursts, with long periods of inactivity between them. These changes in network activity were accompanied by an increase in the frequency and amplitude of miniature postsynaptic excitatory currents, which could be described by a linear up-scaling of current amplitudes. Biochemically, we observed an upregulation in the expression of the glutamate receptor subunit GluA1, but not GluA2, and a strong increase in autophosphorylation of a and b Ca 2þ /calmodulin-dependent protein kinase II (CaMKII), without changes in the levels of kinase expression. These data suggest that a deficit in HSs triggers homeostatic synaptic plasticity and drastically affects functional maturation of neural network.

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“…Evoked spiking activity was averaged over 50 consecutive stimulations applied at a frequency of 0.1 Hz. Data analysis was done using custommade MatLab 2010a scripts (The MathWorks Inc., Natick, MA), except for mean firing rates that were calculated using SPYCODE software (23).…”

Section: Methodsmentioning

confidence: 99%

Functional Interaction between the Scaffold Protein Kidins220/ARMS and Neuronal Voltage-Gated Na+ Channels

Cesca

Satapathy

Ferrea

et al. 2015

Journal of Biological Chemistry

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Background: Tight regulation of ion channel activity is essential for neuronal function. Results: The scaffold protein Kidins220 associates with brain voltage-gated sodium channels and modulates their activity. Conclusion: Lack of Kidins220 in mice causes deregulated sodium channel function in inhibitory neurons, ultimately leading to impaired network excitability. Significance: Kidins220 may help maintain the balance between excitation and inhibition in neural networks.

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Investigating neuronal activity by SPYCODE multi-channel data analyzer

Cited by 121 publications

References 61 publications

Rapid Neuronal Differentiation of Induced Pluripotent Stem Cells for Measuring Network Activity on Micro-electrode Arrays

Rapid Neuronal Differentiation of Induced Pluripotent Stem Cells for Measuring Network Activity on Micro-electrode Arrays

Modulation of network activity and induction of homeostatic synaptic plasticity by enzymatic removal of heparan sulfates

Functional Interaction between the Scaffold Protein Kidins220/ARMS and Neuronal Voltage-Gated Na+ Channels

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