Investigating lymphangiogenesis in a sacrificially bioprinted volumetric model of breast tumor tissue

Liu, Tingting; Liu, Qiong; Anaya, Ingrid; Huang, Di; Kong, Weijia; Mille, Luis Santiago; Zhang, Yu Shrike

doi:10.1016/j.ymeth.2020.04.003

Cited by 33 publications

(47 citation statements)

References 39 publications

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“…Other chips featuring co-cultures of cancer cell lines and LECs demonstrate similar results, usually using cylindrical lumen chips formed by sacrificial molding. [95] Few reports use fibroblasts in their lymphangiogenesischips. [85,89,93] One group designed a micropost device with similar cell compartmentalization as the earlier angiogenesischips where they encapsulated NHLFs across from a monolayer of LECs and observed sprouting.…”

Section: Supporting Cell Typesmentioning

confidence: 99%

“…Some tumor models have even included de novo vascular or sprouting lymphatic networks. [70,76,77,89,95,102] One study showed that the lymphatic networks served as conduits for metastasis via enhanced lymphangiogenesis into the tumor mass. [95] Another showed that both vascular and lymphatic networks increase their sprouting and network formation into tumor masses.…”

Section: A Brief Review Of Vascularized Multicellular Systemsmentioning

confidence: 99%

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Engineering New Microvascular Networks On‐Chip: Ingredients, Assembly, and Best Practices

Tronolone

Jain

2021

Adv Funct Materials

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Tissue engineered grafts show great potential as regenerative implants for diseased or injured tissues within the human body. However, these grafts suffer from poor nutrient perfusion and waste transport, thus decreasing their viability post-transplantation. Graft vascularization is therefore a major area of focus within tissue engineering because biologically relevant conduits for nutrient and oxygen perfusion can improve viability post-implantation. Many researchers used microphysiological systems as testing platforms for potential grafts owing to an ability to integrate vascular networks as well as biological characteristics such as fluid perfusion, 3D architecture, compartmentalization of tissue-specific materials, and biophysical and biochemical cues. Although many methods of vascularizing these systems exist, microvascular self-assembly has great potential for bench-to-clinic translation as it relies on naturally occurring physiological events. In this review, the past decade of literature is highlighted, and the most important and tunable components yielding a self-assembled vascular network on chip are critically discussed: endothelial cell source, tissue-specific supporting cells, biomaterial scaffolds, biochemical cues, and biophysical forces. This paper discusses the bioengineered systems of angiogenesis, vasculogenesis, and lymphangiogenesis and includes a brief overview of multicellular systems. It concludes with future avenues of research to guide the next generation of vascularized microfluidic models.

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Section: Supporting Cell Typesmentioning

confidence: 99%

Section: A Brief Review Of Vascularized Multicellular Systemsmentioning

confidence: 99%

Engineering New Microvascular Networks On‐Chip: Ingredients, Assembly, and Best Practices

Tronolone

Jain

2021

Adv Funct Materials

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“…[ 17 ] Furthermore, in our previous work, the microchannels cultured with lymphatic microvascular ECs were embedded into hydrogels containing breast tumor cells, resulting in the development of a human breast cancer lymphangiogenesis model. [ 18 ] Together, mimicking the interactions between ECs and tumor cells in vitro may help in investigating tumor initiation, cancer pathologies, and therapeutics development.…”

Section: Introductionmentioning

confidence: 99%

Modeling Endothelialized Hepatic Tumor Microtissues for Drug Screening

et al. 2020

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Compared to various traditional 2D approaches, the scaffold‐based 3D tumor models have emerged as an effective strategy to investigate the complex mechanisms behind cancer progression and responses to drug treatments, by providing biomimetic extracellular matrix and stromal‐like microenvironments including the vascular elements. Herein, the development of a 3D endothelialized hepatic tumor microtissue model based on the fusion of multicellular aggregates of human hepatocellular carcinoma cells and human umbilical vein endothelial cells cocultured in poly(lactic‐ co ‐glycolic acid)‐based porous microspheres (PLGA PMs) is reported. In contrast to the conventional 2D culture, the cells within the PLGA PMs exhibit significantly higher half‐maximal inhibitory concentration values against anticancer drugs, including doxorubicin and cisplatin. Furthermore, the feasibility of coculturing other cell types, such as fibroblasts (L929) and HepG2 cells, is investigated. Together, the findings emphasize the significance of engineered 3D hepatic tumor microtissue models using PLGA PM‐based multicellular aggregates for drug screening applications.

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“…Liu et al. developed a human breast tumor model with physiologically relevant embedded lymphatic vessels by sacrificial bioprinting [134] . This offers promise for developing and screening personalized tumor anti-lymphangiogenic therapies in the future.…”

Section: Cancer-specific Bioprinted Models For Precision Chemotherapymentioning

confidence: 99%

3D Bioprinted cancer models: Revolutionizing personalized cancer therapy

Augustine

Kalva

Ahmad

et al. 2021

Translational Oncology

115

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Highlights This review describes how 3D bioprinting can be used for developing patient specific cancer models. Bioprinted cancer models containing patient-derived cancer and stromal cells is promising for personalized cancer therapy screening 3D bioprinted constructs form physiologically relevant cell–cell and cell–matrix interactions. Bioprinted cancer models mimic the 3D heterogeneity of real tumors.

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Investigating lymphangiogenesis in a sacrificially bioprinted volumetric model of breast tumor tissue

Cited by 33 publications

References 39 publications

Engineering New Microvascular Networks On‐Chip: Ingredients, Assembly, and Best Practices

Engineering New Microvascular Networks On‐Chip: Ingredients, Assembly, and Best Practices

Modeling Endothelialized Hepatic Tumor Microtissues for Drug Screening

3D Bioprinted cancer models: Revolutionizing personalized cancer therapy

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