Investigating Lignocellulose in Cornstalk Pretreated with Trametes pubescens Cui 7571 to Improve Enzymatic Saccharification

Abstract

This study investigated the degradation and enzymatic saccharification of cornstalk by white-rot and brown rot fungi. The fungal strains Trametes pubescens Cui 7571, Trametes velutina Dai 10149, and Antrodia wangii Cui 7568 were analyzed in solid-state fermentation cultures. Various extracellular enzyme activities were assessed to determine biochemical changes during the degradation process. Fourier-transform infrared (FTIR) spectroscopy and X-ray diffraction (XRD) analysis were used to determine the structural changes. A correlation analysis between the chemical composition of cornstalk, extracellular enzyme activities, and structural changes indicated that T. pubescens Cui 7571 broke down lignin efficiently and subsequently degraded cellulose, and hemicellulose digestion was not affected by the lignin barrier. Enzymatic hydrolysis demonstrated that the T. pubescens Cui 7571-pretreated samples increased cellulose and hemicellulose conversion in cornstalk. Overall, T. pubescens Cui 7571 displayed excellent performance as a biological pretreatment agent, and lignin played a significant role in the enzymatic saccharification of cornstalk.

Phylogenetic Analysis of Polyporous Fungi Collected from Batam Botanical Garden, Riau Province, Indonesia

“…Trametes ijubarskii and Antrodia wangii are categorized as the most recent known polyporous fungi and their roles has not been extensively studied. However, according to Wu et al (2016) and Thiribhuvanamala et al (2017) both fungi shows hemicellulotic activity under laboratory tests. Other fungal species, Phellinus rimosus is reported to show antitumor activity whereas Earliella scabrosa secretes enzymes useful in degradation of synthetic dyes (Geurra et al, 2008;Ajith & Janardhanan, 2003).…”

Evaluation of Crystalline Cellulose of Corn Straw through Different Pretreatments Via X-Ray Diffraction, Scanning Electron Microscopy and Infrared Spectroscopy

“…Numerous pretreatments methods of corn stalk have been reported ， but few people pay attention to the crystallinity of crystalline cellulose (Behera et al, 2014;Rastogi et al, 2017;Hassan et al,2018;Huang et al,2018;Kojima et al,2019;Ponnusamy et al,2019;Xu et al,2019) The study compared of four pretreatments methods that is oven drying, steaming, complex enzymolysis and fermentation by Candida utilis and Pachysolen tannophilus, evaluating the crystallinity of crystalline cellulose after treated with the aid of X-ray diffraction, scanning electron microscopy and infrared spectroscopy. At the same time, the corn stalks's utilization rate of reducing sugar and the output of protein feed have been increased (Chen and Zhang, 2008;Luo et al, 2009;Chen et al, 2009;Yang et al, 2015;Wu et al, 2016;Chen et al, 2018). Thus, comparing the structure of fibrous crystal and crystallinity can provide certain theory reference for improving the high utilization rate of corn stalk.…”