Investigating Cellulose Binding of Peptides Derived from Carbohydrate Binding Module 1
Annika Lill,
Alexandra Herbst,
Markus Langhans
et al.
Abstract:Carbohydrate-binding modules (CBM) have emerged as useful
tools
for a wide range of tasks, including the use as purification tags
or for cellulose fiber modification. For this purpose, the CBM needs
to be attached to a target protein leading to large constructs. We
investigated if short peptides from the carbohydrate binding site
of CBMs can bind in a similar way as native, full-length CBMs to nanocrystalline
cellulose (NCC) or cotton linter paper. We designed our cellulose-binding
peptides to be less hydropho… Show more
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