Investigating cellular stress response to heat stress in the seagrass Posidonia oceanica in a global change scenario

Traboni, Claudia; Mammola, Salvatore Davide; Ruocco, Miriam; Ontoria, Yaiza; Ruiz, Juan Manuel García; Procaccini, Gabriele; Marín-Guirao, Lázaro

doi:10.1016/j.marenvres.2018.07.007

Cited by 39 publications

(29 citation statements)

References 88 publications

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“…Interestingly, transcripts with a role in protein repair, as chaperones and chaperone regulators, were generally overexpressed in LL, as were those related to the response to DNA damage, whereas transcripts related to proteolysis were downregulated. This mirrors what previously reported in P. oceanica under heat stress, where CSR appeared to bypass the intermediate proteolysis‐related pathway, suggesting that molecular chaperoning, DNA repair and apoptosis inhibition processes are the instant stress signals exerted by the species (Traboni et al., 2018).…”

Section: Discussionsupporting

confidence: 88%

A king and vassals' tale: Molecular signatures of clonal integration in Posidonia oceanica under chronic light shortage

et al. 2020

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GO ID GO-Biological process FDR Apical 0080037 negative regulation of cytokinin-activated signalling pathway 3.31E-06 0009737 response to abscisic acid 5.52E-04 0046148 pigment biosynthetic process 1.13E-03 2000762 regulation of phenylpropanoid metabolic process 1.28E-03 0033386 geranylgeranyl diphosphate biosynthetic process 6.78E-03 0006857 oligopeptide transport 9.24E-03 0015698 inorganic anion transport 1.23E-02 0043693 monoterpene biosynthetic process 1.40E-02 0003006 developmental process involved in reproduction 2.22E-02 0033384 geranyl diphosphate biosynthetic process 4.20E-02 0009791 post-embryonic development 4.43E-02 0009269 response to desiccation 4.53E-02 0009812 flavonoid metabolic process 4.86E-02 Vertical 0006464 cellular protein modification process 2.58E-04 0006355 regulation of transcription, DNA-templated 7.44E-04 0006857 oligopeptide transport 9.75E-04 0009414 response to water deprivation 4.27E-03 0033554 cellular response to stress 6.28E-03 0080037 negative regulation of cytokinin-activated signalling pathway 9.12E-03 0048316 seed development 1.12E-02 0060560 developmental growth involved in morphogenesis 1.18E-02 0032101 regulation of response to external stimulus 1.29E-02 0016310 Phosphorylation 1.46E-02 0008202 steroid metabolic process 1.50E-02 0006970 response to osmotic stress 1.85E-02 0048588 developmental cell growth 1.89E-02 0090408 phloem nitrate loading 2.53E-02 0031347 regulation of defense response 2.67E-02 0019748 secondary metabolic process 2.67E-02 0009723 response to ethylene 2.74E-02 1901615 organic hydroxy compound metabolic process 2.81E-02 0043090 amino acid import 2.95E-02 0048437 floral organ development 3.07E-02 2000026 regulation of multicellular organismal development 3.18E-02 0035524 proline transmembrane transport 3.18E-02 0045862 positive regulation of proteolysis 3.55E-02 0051254 positive regulation of RNA metabolic process 3.61E-02 0065008 regulation of biological quality 3.64E-02 0015810 aspartate transmembrane transport 3.87E-02 0010628 positive regulation of gene expression 3.87E-02 0006868 glutamine transport 4.11E-02 0048235 pollen sperm cell differentiation 4.11E-02 0015827 tryptophan transport 4.11E-02 0048545 response to steroid hormone 4.27E-02 0015825 L-serine transport 4.34E-02 0009556 microsporogenesis 4.50E-02 0044085 cellular component biogenesis 4.71E-02 0006955 immune response 4.89E-02 0006996 organelle organization 4.93E-02 1.22E-04 0010114 response to red light 4.62E-03 0009860 pollen tube growth 1.24E-04 0007080 mitotic metaphase plate congression 4.83E-03 0010192 mucilage biosynthetic process 1.29E-04 0010889 regulation of sequestering of triglyceride 5.00E-03 0016259 selenocysteine metabolic process 1.87E-04 0048653 anther development 5.19E-03 2000022 regulation of jasmonic acid mediated signalling pathway 1.90E-04 0031048 chromatin silencing by small RNA 5.29E-03 0000090 mitotic anaphase 2.08E-04 0070370 cellular heat acclimation 5.41E-03 0009750 response to fructose 2.99E-04 0010264 myo-inositol hexakisphosphate biosynthetic process 5.41...

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Section: Discussionsupporting

confidence: 88%

A king and vassals' tale: Molecular signatures of clonal integration in Posidonia oceanica under chronic light shortage

et al. 2020

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“…In this regard, based on the thermal sensitivity of this species to high temperatures, as described above [19,77,102], our results and the findings of other studies [27,76,103,104], we suggest a thermal threshold for P . oceanica of between 30°C and 35°C.…”

Section: Discussionsupporting

confidence: 86%

The negative effects of short-term extreme thermal events on the seagrass Posidonia oceanica are exacerbated by ammonium additions

et al. 2019

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Global warming is increasingly affecting our biosphere. However, in addition to global warming, a panoply of local stressors caused by human activities is having a profound impact on our environment. The risk that these local stressors could modify the response of organisms to global warming has attracted interest and fostered research on their combined effect, especially with a view to identifying potential synergies. In coastal areas, where human activities are heavily concentrated, this scenario is particularly worrying, especially for foundation species such as seagrasses. In this study we explore these potential interactions in the seagrass Posidonia oceanica. This species is endemic to the Mediterranean Sea. It is well known that the Mediterranean is already experiencing the effects of global warming, especially in the form of heat waves, whose frequency and intensity are expected to increase in the coming decades. Moreover, this species is especially sensitive to stress and plays a key role as a foundation species. The aim of this work is thus to evaluate plant responses (in terms of photosynthetic efficiency and growth) to the combined effects of short-term temperature increases and ammonium additions.To achieve this, we conducted a mesocosm experiment in which plants were exposed to three thermal treatments (20°C, 30°C and 35°C) and three ammonium concentrations (ambient, 30 μM and 120 μM) in a full factorial experiment. We assessed plant performance by measuring chlorophyll fluorescence variables (maximum quantum yield (Fv/Fm), effective quantum yield of photosystem II (ΔF/Fm’), maximum electron transport rate (ETRmax) and non-photochemical quenching (NPQ)), shoot growth rate and leaf necrosis incidence. At ambient ammonium concentrations, P. oceanica tolerates short-term temperature increases up to 30°C. However, at 35°C, the plant loses functionality as indicated by a decrease in photosynthetic performance, an inhibition of plant growth and an increase of the necrosis incidence in leaves. On the other hand, ammonium additions at control temperatures showed only a minor effect on seagrass performance. However, the combined effects of warming and ammonium were much worse than those of each stressor in isolation, given that photosynthetic parameters and, above all, leaf growth were affected. This serves as a warning that the impact of global warming could be even worse than expected (based on temperature-only approaches) in environments that are already subject to eutrophication, especially in persistent seagrass species living in oligotrophic environments.

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“…They were significantly over-expressed in all leaf segments selected along P. oceanica leaves, with the basal portion of leaf 1 (1B) featuring the highest fold changes in respect to controls. The induction of AOX and BI under heat stress confirms their pivotal role in mediating seagrass stress acclimation (Marín-Guirao et al, 2017; Tutar et al, 2017; Traboni et al, 2018). Aox pathway minimizes heat shock-mediated ROS production across the mitochondrial electron-transport chain (Vanlerberghe, 2013), while Bax Inhibitor acts preventing ROS-induced programmed cell death (Watanabe and Lam, 2006).…”

Section: Discussionmentioning

confidence: 57%

Differential Leaf Age-Dependent Thermal Plasticity in the Keystone Seagrass Posidonia oceanica

et al. 2019

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Introduction: Gene-expression patterns and their upstream regulatory mechanisms (e.g. epigenetic) are known to modulate plant acclimatability and thus tolerance to heat stress. Within species, thermal plasticity (i.e. temperature-sensitive phenotypic plasticity) and differential thermo-tolerance are recognized among different genotypes, development stages, organs or tissues. Leaf age and lifespan have been demonstrated to strongly affect photosynthetic thermo-tolerance in terrestrial species, whereas there is no information available for marine plants. Materials and Methods: Here, we investigated how an intense warming event affects molecular and photo-physiological functions in the large-sized seagrass Posidonia oceanica, at fine spatial resolution. Plants were exposed for one week at 34°C in a controlled-mesocosm system. Subsequent variations in the expression of 12 target genes and global DNA methylation level were evaluated in three leaf-age sections (i.e. basal, medium and high) established along the longitudinal axis of youngest, young and fully mature leaves of the shoot. Targeted genes were involved in photosynthesis, chlorophyll biosynthesis, energy dissipation mechanisms, stress response and programmed cell death. Molecular analyses paralleled the assessment of pigment content and photosynthetic performance of the same leaf segments, as well as of plant growth inhibition under acute warming. Results: Our data revealed, for the first time, the presence of variable leaf age-dependent stress-induced epigenetic and gene-expression changes in seagrasses, underlying photo-physiological and growth responses to heat stress. An investment in protective responses and growth arrest was observed in immature tissues; while mature leaf sections displayed a higher ability to offset gene down-regulation, possibly through the involvement of DNA methylation changes, although heat-induced damages were visible at photo-physiological level. Discussion: Overall, mature and young leaf tissues exhibited different strategies to withstand heat stress and thus a variable thermal plasticity. This should be taken in consideration when addressing seagrass response to warming and other stressors, especially in large-sized species, where sharp age differences are present within and among leaves, and other gradients of environmental factors (e.g. light) could be at play. Molecular and physiological evaluations conducted only on adult leaf tissues, as common practice in seagrass research, could give inadequate estimates of the overall plant state, and should not be considered as a proxy for the whole shoot.

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Investigating cellular stress response to heat stress in the seagrass Posidonia oceanica in a global change scenario

Cited by 39 publications

References 88 publications

A king and vassals' tale: Molecular signatures of clonal integration in Posidonia oceanica under chronic light shortage

A king and vassals' tale: Molecular signatures of clonal integration in Posidonia oceanica under chronic light shortage

The negative effects of short-term extreme thermal events on the seagrass Posidonia oceanica are exacerbated by ammonium additions

Differential Leaf Age-Dependent Thermal Plasticity in the Keystone Seagrass Posidonia oceanica

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