Investigating Cellular Responses During Photohydrogen Production by the Marine Microalga Tetraselmis subcordiformis by Quantitative Proteome Analysis

Abstract

The marine microalga Tetraselmis subcordiformis could photoproduce hydrogen under the regulation of carbonyl cyanide m-chlorophenylhydrazone (CCCP), and a hydrogen production process kinetic analysis was characterized by two peaks, suggesting that two distinct mechanisms might exist in this alga. Therefore, 2D nanoliquid chromatography-tandem mass spectrometry (LC-MS/MS) was introduced to analyze the proteome of samples from different time points. A total of 912 proteins were identified, providing a global view of the cellular responses at the proteomic level. These proteins can be divided into multiple functional groups including stress responses, energy metabolism and redox homeostasis. The quantitative proteomic data provided more details on the electron donors for hydrogen production. During the first stage, photosystem II produced electrons for hydrogen production; during the second stage, metabolites were the major electron donors via nonphotochemical plastoquinone reduction by NADH dehydrogenase.

Extracting protein from microalgae (Tetraselmis chuii) for proteome analysis

“…1 ). In disruption method I (DM I) eight different extraction solutions were tested ( A-H ) [ 12 , 25 ], two different types of beads were used, and two different disruption cycles were applied [29] . In DM II and III different extraction solutions were tested ( A, D and B, C , respectively ) .…”

“…Nonetheless, although protein extraction efficiency is an important parameter it is not correlated with protein integrity an important factor for bioactivity and proteomics analysis. Indeed, the reported microalgae protein extraction methods are for analytical and functional purposes, and few have focused on the proteome [ 12 , 13 , [15] , [16] , [17] , [22] , [23] , [24] , [25] , [26] ]. In proteomics studies it is essential to use protein extracts that give representative and consistent, high-quality results.…”

“…Interest in CCFM03 is high and it was the first strain of the Tetraselmis genus to be marketed, for food and nutraceutical applications [27] . However, the potential of the genus is still under explored and limited information exists about the most appropriate protein extraction methods and the proteome profile [ 22 , 25 , 26 , 28 ]. Choosing a suitable cell disruption treatment prior to extraction is one of the most vital steps for downstream processing.…”

Omics‐prediction of bioactive peptides from the edible cyanobacterium Arthrospira platensis proteome

“…Finally, the peptides were stored at −20 °C. Detailed procedures are reported elsewhere . It should be noted that in this study the digested peptides were not labeled.…”

Use of a lipid rich strain reveals mechanisms of nitrogen limitation and carbon partitioning in the haptophyte Tisochrysis lutea

“…In microalgae, proteomics have provided research contributions in the areas of cell biology [50][51][52][53], metabolism [17,[54][55][56], stress and signaling [57][58][59][60], evolution [61][62][63] and biotechnology [26,54,[64][65][66][67]. Two-dimensional gel electrophoresis (2-DE) followed by protein identification by mass spectrometry is the main technique for comparative analysis in microalgae, but label free analysis are increasingly being used, notably for the study of phosphorus and nitrogen availability [8,[68][69][70][71].…”