Investigating Bacterial Diversity Along Alkaline Hot‐Spring Thermal Gradients by Barcoded Pyrosequencing

Miller, Scott R.; Weltzer, Michael L.

doi:10.1002/9781118010549.ch41

Cited by 2 publications

(1 citation statement)

References 17 publications

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“…Thaumarchaeota and Crenarchaeota were present in both sites, but Euryarchaeota were only found in the acidic hot spring (Wemheuer et al, 2013 ). In other reports, the dominant genera found in alkaline hot springs were those of the Thermus (De León et al, 2013 ), Hydrogenobacter (Hou et al, 2013 ), Caldicellulosiruptor, Dictyoglomus, Fervidobacterium (Sahm et al, 2013 ), and Synechococcus (Miller and Weltzer, 2011 ) genera. Among the Archaea, the Crenarchaeal orders Desulfurococcales and Thermoproteales often predominate in alkaline hot springs (Hou et al, 2013 ; Sahm et al, 2013 ).…”

Section: Introductionmentioning

confidence: 85%

Diversity of thermophiles in a Malaysian hot spring determined using 16S rRNA and shotgun metagenome sequencing

Chan

Tay³

et al. 2015

Front. Microbiol.

150

120

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The Sungai Klah (SK) hot spring is the second hottest geothermal spring in Malaysia. This hot spring is a shallow, 150-m-long, fast-flowing stream, with temperatures varying from 50 to 110°C and a pH range of 7.0–9.0. Hidden within a wooded area, the SK hot spring is continually fed by plant litter, resulting in a relatively high degree of total organic content (TOC). In this study, a sample taken from the middle of the stream was analyzed at the 16S rRNA V3-V4 region by amplicon metagenome sequencing. Over 35 phyla were detected by analyzing the 16S rRNA data. Firmicutes and Proteobacteria represented approximately 57% of the microbiome. Approximately 70% of the detected thermophiles were strict anaerobes; however, Hydrogenobacter spp., obligate chemolithotrophic thermophiles, represented one of the major taxa. Several thermophilic photosynthetic microorganisms and acidothermophiles were also detected. Most of the phyla identified by 16S rRNA were also found using the shotgun metagenome approaches. The carbon, sulfur, and nitrogen metabolism within the SK hot spring community were evaluated by shotgun metagenome sequencing, and the data revealed diversity in terms of metabolic activity and dynamics. This hot spring has a rich diversified phylogenetic community partly due to its natural environment (plant litter, high TOC, and a shallow stream) and geochemical parameters (broad temperature and pH range). It is speculated that symbiotic relationships occur between the members of the community.

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