Investigating Advanced Approaches Based on Iso-Absorptivity Coefficient in Unresolved Spectral Signals of Binary Mixtures

Lotfy, Hayam M.; Saleh, Sarah S.

doi:10.1155/2019/7924821

Cited by 16 publications

(6 citation statements)

References 21 publications

(22 reference statements)

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“…According to aforementioned points, the D0 spectrum of each PHN and BEN were attained, therefore the concentration of the two compounds were acquired via their regression equations at their corresponding λMax. As a result, obtaining the zero order spectra by this method acts as a fingerprint spectrum which represent the main advantage of this method and allowed testing the purity of both components [32]. On the other hand, this method is considered as a multi-step technique.…”

Section: Resultsmentioning

confidence: 99%

“…Absorptivity Centering (a-centering) method was recently discovered and applied for the determination of two components (X and Y) representing an iso-absorptive point crossing with partly or totally overlapping spectra. through this technique the zero order spectrum of the two components could be obtained and as a result a spectral profile and purity of the peak of each component were achieved [31, 32, 33, 34].…”

Section: Introductionmentioning

confidence: 99%

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New spectral resolution techniques for resolving and determining the components in binary fixed-dose combinations

Zakri

Obaydo

Sakur

2019

Heliyon

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Four spectrophotometric approaches were performed to determine a binary combination of Phenazone and Benzocaine in pure powder form and in pharmaceutical formations. This investigation submits the application of four techniques contingent on the presence of the extended area of the spectra of one compound in the binary mixture, these methods include Absorptivity Centering (a-centering), Absorbance Subtraction (AS), Amplitude Modulation (AM) and Concentration Value (CV). The linearity range for the above-mentioned approaches was found to be 3.0–15.0 μg/mL for Benzocaine in a-centering method and 3.0–30.0 μg/mL for Benzocaine and Phenazone in other advanced methods. The four techniques were evaluated as per ICH criteria and were successfully utilized for the determination of Phenazone and Benzocaine existing in pharmaceutical formulations. All results gained by the submitted approaches were statistically compared with a previously published method, and no important differences were detected.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

New spectral resolution techniques for resolving and determining the components in binary fixed-dose combinations

Zakri

Obaydo

Sakur

2019

Heliyon

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“…Lotfy and Omran [29] recently defined the factorized spectrum of Y as “a special spectrum that has absorbance value equal to one at λ iso which is acquired by dividing the D 0 spectra of Y by its correlated absorbance value estimated at λ iso .” Also, the factorized spectrum is applied for the analysis of other mixtures [30, 31]:FS′=AYAYλiso,FS′=aYCYaYλisoCY,FS′=aYaYλiso.…”

Section: Theoretical Backgroundmentioning

confidence: 99%

Fingerprint Spectrophotometric Methods for the Determination of Co-Formulated Otic Solution of Ciprofloxacin and Fluocinolone Acetonide in Their Challengeable Ratio

Obaydo

Sakur

2019

Journal of Analytical Methods in Chemistry

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Six spectrophotometric methods were developed to determine a new single-dose otic solution known as “Otovel®,” which consists of two components: the major one is ciprofloxacin (CIP) and the minor is fluocinolone acetonide (FLU). The ratio of (CIP) and (FLU) in Otovel® is 12 : 1, which is considered a challengeable ratio for UV determination. Thus, spectrum addition as a sample enrichment technique was required for the analysis of (FLU) low concentration. All these methods were capable of resolving the spectra for each component in D0 belonging to the fingerprint resolution technique. The former absorptivity centering (a-centering) method was recently developed in 2018; it was effectively applied for its solution of both binary components in Otovel®, while another method, ratio subtraction (RS), is considered as an original resolution method that could be applied to determine only one component in mixtures. However, the other four methods that are related to their original method (RS) were extended ratio subtraction (EXRS), constant multiplication (CM), unified constant subtraction (UCS), and spectrum subtraction (SS). They were also easily applied for completing the quantification of binary mixture drugs present in Otovel®. The linearity ranges were found to be 3.0–15.0 μg/mL for (CIP) and (FLU), respectively. All results acquired from the proposed methods were successfully estimated according to ICH criteria and were statistically compared with official ones where no differences were noticed.

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“…On the other hand, chromatographic methods transcend in the resolution and quantitation of active ingredients in different matrix, while interference of other components or excipients is not easily resolved using spectroscopic approaches as in HPLC. So, upon performing quantitation of multi-component matrix using spectroscopic approach, certain manipulations should be applied [32][33][34] .…”

Section: Introductionmentioning

confidence: 99%

Validated green spectroscopic manipulation of area under the curve (AUC) for estimation of Simvastatin: Application to nano-structured lipid carriers and niosomal systems

MANNAA¹,

GAZAYERLY²,

ABDELBARY³

et al. 2023

jrp

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This work introduced a green spectrophotometric method based on the manipulation of the area under the curve named Area under the curve correction method (AUCCM). The method was applied for the characterization and evaluation the in-vitro release of two nanocarrier systems namely, nano-structured lipid carrier (NLC) and niosomal system of Simvastatin (SIM), through measuring free SIM in presence of the possible excipients/interferents (oleic acid and cholesterol) in a challenging matrix. The estimation of SIM was calculated using the area under the curve (AUC) in the wavelength range of (234.5 -245.5) nm which includes the λmax of SIM in the concentration range of (1-35 µg/mL). The method was validated in compliance with ICH guidelines. The greenness of the developed analytical method will be assessed and compared to other reported methods using the greenness assessment tools known as: National Environmental Methods Index (NEMI), Green Analytical Procedure Index (GAPI) and Eco-Scale. The method proved to be the best regrading greenness and and was successfully applied for the in-vitro release study of SIM from the two nano carrier systems.

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Investigating Advanced Approaches Based on Iso-Absorptivity Coefficient in Unresolved Spectral Signals of Binary Mixtures

Cited by 16 publications

References 21 publications

New spectral resolution techniques for resolving and determining the components in binary fixed-dose combinations

New spectral resolution techniques for resolving and determining the components in binary fixed-dose combinations

Fingerprint Spectrophotometric Methods for the Determination of Co-Formulated Otic Solution of Ciprofloxacin and Fluocinolone Acetonide in Their Challengeable Ratio

Validated green spectroscopic manipulation of area under the curve (AUC) for estimation of Simvastatin: Application to nano-structured lipid carriers and niosomal systems

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