Investigating Adsorption/Desorption of DNA on ZIF-8 Surface by Fluorescently Labeled Oligonucleotides

Abstract

As an important subclass of MOFs, ZIF-8, built from 2-methylimidazole and Zn­(NO3)2·6H2O, possesses excellent biocompatibility and high stability in aqueous solution. Recently, it has been found that ZIF-8 can efficiently adsorb DNA and quench the adsorbed fluorophores to a large extent. These properties make it possible to prepare DNA-based optical sensors using ZIF-8. Although practical analytical applications are being demonstrated, the basic understanding of the binding between ZIF-8 and DNA in solution has received relatively little attention. In this work, we report that the adsorption of 12-, 18-, 24-, and 36-mer single-stranded DNAs on ZIF-8 are affected by several factors. It is found from the outcomes that shorter DNAs are adsorbed more rapidly to the surface of ZIF-8. On the other hand, desorption of the probe DNA can be achieved using complementary strand DNA to restore the fluorescence value. Furthermore, the salt contributes to adsorption to some extent. These findings are important for further understanding of the interactions between DNA and ZIF-8 and for the optimization of DNA and MOF-based devices and sensors.

Peptide-Mediated Synthesis of Zeolitic Imidazolate Framework-8: Effect of Molecular Hydrophobicity, Charge Number and Charge Location

“…Metal-organic frameworks (MOFs) are a novel class of crystallized porous materials that are self-assembled by metal clusters as connecting points and organic ligands as bridging molecules to form a two- or three-dimensional framework through coordination bonds [ 1 , 2 , 3 , 4 , 5 , 6 , 7 ]. MOFs have attracted great interests in recent years by having remarkable features such as adjustable structure, high porosity, large specific surface area, good modifiability and excellent stability and have been used widely in many areas, for example, adsorption [ 8 , 9 , 10 , 11 , 12 , 13 ], catalysis [ 14 , 15 , 16 , 17 , 18 ], sensing [ 19 , 20 , 21 , 22 , 23 ], drug delivery [ 24 , 25 , 26 , 27 , 28 , 29 , 30 ] and gas storage and separation [ 31 , 32 , 33 ], etc. Besides the intrinsic properties of MOFs, their morphology and size also play crucial roles for their practical applications.…”

Rapid template‐guided ligand‐free synthesis of ultrasmall Pt nanoclusters with efficient hydrogen evolution reaction activity and their versatile release

“…The extraction mechanism of PtNCs from ZIF‐8 in alkaline solution may be attributed to the disruption of hydrophobic interaction of ZIF‐8 as triggered by the sonication. [ 69–71 ]…”

Platform Formed from ZIF-8 and DNAzyme: “Turn-On” Fluorescence Assay for Simple, High-Sensitivity, and High-Selectivity Detection of Pb2+

“…22 In recent years, several MOFs have been utilized as quenchers toward dye-labeled DNA, such as MIL-101, 23 ZrMOF, 24 UiO-66-NH 2 , 25 and ZIF-8. 26 Compared with conventional 1D/2D materials, such as gold nanoparticles (AuNPs), graphene oxide (GO), molybdenum disulfide (MoS 2 ), and tungsten disulfide (WS 2 ), three-dimensional (3D) MOFs have large surface areas, diverse structures, and unique physical and chemical properties. 25 ZIF-8, as one of the promising MOFs, can be obtained by the nucleation growth of Zn 2+ and 2-methylimidazole 27 and has obvious advantages of highly tunable capability, simple synthesis, large surface area, excellent biocompatibility, lowcost precursors, low toxicity, and highly conducting nature.…”