Investigate the Toxoplasma gondii infection in the consumed beef in Al-Diwaniyah province

Abstract

Toxoplasmosis is very important zoonotic disease in the world cause by an obligate intracellular protozoan parasite called Toxoplasma gondii can infect human and all warm-blood animals, beef consider from most important source for infection with T. gondii and there is no really data and study about the rate of the infection in beef in Al-Diwaniyah province, so for this reason the aim of this work was designed. A total of 300 samples which collected from heart, tongue, muscles, of 100 slaughtered beef of local and imported cattle, throughout the period from September 2017 to May 2018, initially examined microscopically for searching on T. gondii bradyzoites then all suspected samples was subjected to conventional PCR technique through B1gene amplification to confirm the infection, in addition to analyzed the recorded data for each sample to determine the effect of some factors on prevalence of infection like organ, season and animal age. Out of 300 tested samples only 53 were confirm positive T. gondii DNA. The infection in local beef was higher (22%) than in imported (13.5%), while there is no difference in infection among different examined organs. Regarding to effect of some factors, the autumn season recorded highest rate of infection with significant differences rather than others seasons in both local and imported beef, whereas, age appears with no effect on infection. The local cattle meat is riskier than the imported due to the higher rate of infection with T. gondii, and the animal age cannot affect on the infection rate, in comparing with the season which play role in this rate.

Molecular detection and seroprevalence of Toxoplasmosis in free range local chickens (Gallus domesticus) in Duhok province, Iraq

“…Besides, evidence found that infection rate of T. gondii in free range chickens of different part of the world range from 2%-100% (21). The great variations was found as a result of differences in climate changes which has great impact on the lifespan of oocysts, or differences in the used diagnostic techniques, the kind of the samples were depended and seasonality (22)(23)(24).…”

Seroprevalence of Neospora caninum infections in cattle in Kirkuk province

“…Perhaps the difference is due to the geographical area or the surrounding environment and topographical reasons that affect the development and survival of oocysts (20). Many factors may affect the seroprevalence of parasites including sample size, environmental differences age and breed of cattle (21,22). Infectious materials, like fetuses prone to miscarriage, dead calves and fetal membranes can be essential sources of dogs infected.…”

Immunohistochemical study of TGF-β, PDL-1, and IL-10 in patients with Toxoplasma gondii

“…Bradyzoite or slow-multiplying form (brady = slow in Greek), multiplying within a tissue cyst. Bradyzoites are also called cystozoites [28]. Its multiply by endodyogeny, which lies inside tissue cyst [29,30].…”