Investigação das atividades analgésica e antiinflamatória do extrato metanólico dos rizomas de Echinodorus grandiflorus

Abstract

RESUMO:O presente trabalho investigou as atividades analgésica e antiinfl amatória do extrato metanólico de rizomas de Echinodorus grandifl orus. Para isto, foram realizados os testes de contorções abdominais induzidas por ácido acético; tempo da lambida da pata induzida por formalina; edema de pata e pleurisia induzidas por carragenina. As doses de 100, 200 e 400 mg/kg inibiram as contorções em 34,30%, 35,84% e 36,07%, respectivamente. O tempo de lambida da primeira fase foi reduzido nas doses de 200 e 400 mg/kg, enquanto a segunda fase teve redução em 400 mg/kg. As doses testadas diminuíram o edema, enquanto o volume do exsudato foi reduzido em 24,61% na dose de 400 mg/kg. As doses de 200 e 400 mg/kg diminuíram a migração leucocitária. Os resultados indicam que o extrato de E. grandifl orus testado pode constituir alvo potencial para uso em terapias da dor e da infl amação. Unitermos:Echinodorus grandifl orus, atividade analgésica, atividade antiinfl amatória.ABSTRACT: "Investigation of analgesic and anti-infl ammatory activities of Echinodorus grandifl orus rhizomes methanol extract". The present work investigated the analgesic and antiinfl ammatory activities of the methanol extract of the rhizomes of Echinodorus grandifl orus. For this purpose, acetic acid writhing, paw licking induced by formalin, carrageenan-induced rat paw edema and pleurisy tests were performed. The doses of 100, 200 and 400 mg/kg inhibited the contortions 34.30%, 35.84% and 36.07%, respectively. The lick of the paw 1st phase was reduced at the doses of 200 and 400 mg/kg, while the 2nd phase had reduction at 400 mg/kg. All doses inhibited the paw edema, while the volume of the exsudate was reduced 24.61% in the dose of 400 mg/kg. The doses of 200 and 400 mg/kg decreased the leukocytes migration. The results indicate that the methanol extract of E. grandifl orus can constitute target potential for use in therapies of the pain and infl ammation.

Trans-Aconitic acid, glucosylflavones and hydroxycinnamoyltartaric acids from the leaves of Echinodorus grandiflorus ssp. aureus, a Brazilian medicinal plant

“…The leaves are used in traditional Brazilian medicine for their diuretic and antirheumatic properties (Tanaka et al, 1997;Agra et al, 2007). Recently, a methanol extract of E. grandifl orus was found to have antimicrobial, analgesic and antiinfl ammatory activity (De Souza et al, 2004;Dutra et al, 2006). Unpublished data by Cechinel Filho (Univali, Itajai-SC, Brazil) identifi ed caffeic acid, ß-sitosterol, and the fl avones swertisin and swertiajaponin as constituents.…”

Effect of the Hydroethanolic Extract from Echinodorus grandiflorus Leaves and a Fraction Enriched in Flavone-C-Glycosides on Antigen-Induced Arthritis in Mice

“…Micheli (Alismataceae) is a medicinal plant popularly known as "chapéu-de-couro" in Brazil, its leaves being traditionally used to treat inflamma-tory conditions such as osteoarthritis and rheumatoid arthritis [5]. The anti-inflammatory activity of the species has been demonstrated for a methanol extract from the rhizomes employing carrageenan-induced edema in rats [6] as well as for the n-hexane, methanol, and aqueous extracts from the leaves using mice paw edema induced by compound 48/80, histamine, and serotonin [7]. In a previous work, we reported flavone-Cglycosides (e.g., swertisin, swertiajaponin, isoorientin, and isovitexin), diterpenes, and aconitic acid as the antiedematogenic constituents of the species [8].…”

In Vitro TNF-α Inhibition Elicited by Extracts from Echinodorus grandiflorus Leaves and Correlation with Their Phytochemical Composition

“…Different extracts of E. grandiflorus have been described as possessing anti-inflammatory activity, including the methanol extract of rhizomes [8], as well as the n-hexane, methanol, hydroalcoholic, and aqueous extracts of leaves [7,9,10]. In fact, E. grandiflorus is traditionally used as an anti-arthritic agent due to its alleged anti-inflammatory activity.…”