“Inverted Microcarriers” for Cell Cultures Made by Polymerization of Shells around Agarose Microspheres in a Non-Cytotoxic Procedure

Cadic, Ch.; Baquey, C.; Dupuy, B.

doi:10.1295/polymj.23.933

Cited by 5 publications

(3 citation statements)

References 10 publications

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“…These matrices, when used in a bioreactor under agitation, offer a three-dimensional system for the scalable processing of anchorage-dependent cells, specifically hMSCs for cell therapeutic applications. 17,18 MCs have been prepared from a wide variety of polymeric materials, 19,20 including polyethylene, 21 polystyrene, 17,22 polydimethylsiloxane rubber, 23 acrylamide polymer, 24 cellulose, 25 and biodegradable polymers such as chitosan, 26 glycosaminoglycans, 27 dextran, 28 collagen (gelatin), 29,30 and polyester. 31 Poly-ε-caprolactone (PCL) is a bioresorbable polymer that has been used in human implants, 32−34 with an emphasis on bone regeneration, where it advantageously generates minimal acidic by-products from its degradation.…”

Section: Introductionmentioning

confidence: 99%

“…MCs have been prepared from a wide variety of polymeric materials, , including polyethylene, polystyrene, , polydimethylsiloxane rubber, acrylamide polymer, cellulose, and biodegradable polymers such as chitosan, glycosaminoglycans, dextran, collagen (gelatin), , and polyester . Poly-ε-caprolactone (PCL) is a bioresorbable polymer that has been used in human implants, − with an emphasis on bone regeneration, where it advantageously generates minimal acidic by-products from its degradation.…”

Section: Introductionmentioning

confidence: 99%

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Tunable Volumetric Density and Porous Structure of Spherical Poly-ε-caprolactone Microcarriers, as Applied in Human Mesenchymal Stem Cell Expansion

Lam

Toh

et al. 2017

Langmuir

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Polymeric microspheres may serve as microcarrier (MC) matrices, for the expansion of anchorage-dependent stem cells. They require surface properties that promote both initial cell adhesion and the subsequent spreading of cells, which is a prerequisite for successful expansion. When implemented in a three-dimensional culture environment, under agitation, their suspension under low shear rates depends on the MCs having a modest negative buoyancy, with a density of 1.02-1.05 g/cm. Bioresorbable poly-ε-caprolactone (PCL), with a density of 1.14 g/cm, requires a reduction in volumetric density, for the microspheres to achieve high cell viability and yields. Uniform-sized droplets, from solutions of PCL dissolved in dichloromethane (DCM), were generated by coaxial microfluidic geometry. Subsequent exposure to ethanol rapidly extracted the DCM solvent, solidifying the droplets and yielding monodisperse microspheres with a porous structure, which was demonstrated to have tunable porosity and a hollow inner core. The variation in process parameters, including the molecular weight of PCL, its concentration in DCM, and the ethanol concentration, served to effectively alter the diffusion flux between ethanol and DCM, resulting in a broad spectrum of volumetric densities of 1.04-1.11 g/cm. The solidified microspheres are generally covered by a smooth thin skin, which provides a uniform cell culture surface and masks their internal porous structure. When coated with a cationic polyelectrolyte and extracellular matrix protein, monodisperse microspheres with a diameter of approximately 150 μm and densities ranging from 1.05-1.11 g/cm are capable of supporting the expansion of human mesenchymal stem cells (hMSCs). Validation of hMSC expansion was carried out with a positive control of commercial Cytodex 3 MCs and a negative control of uncoated low-density PCL MCs. Static culture conditions generated more than 70% cell attachment and similar yields of sixfold cell expansion on all coated MCs, with poor cell attachment and growth on the negative control. Under agitation, coated porous microspheres, with a low density of 1.05 g/cm, achieved robust cell attachment and resulted in high cell yields of ninefold cell expansion, comparable with those generated by commercial Cytodex 3 MCs.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Tunable Volumetric Density and Porous Structure of Spherical Poly-ε-caprolactone Microcarriers, as Applied in Human Mesenchymal Stem Cell Expansion

Lam

Toh

et al. 2017

Langmuir

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“…119 Finally, similar in vitro results were obtained when PEG or poly(vinyl-alcohol) (PVA) was chemically grafted to PLL adsorbed on alginate capsules 120 or when PEG was adsorbed on PLL or chitosan tetra-layered alginate particles. 121 Dupuy et al prepared pancreatic islets in agarose solution with extrusion to form beads, which were coated by in situ polymerization of an acrylamide/biacrylamide gel, 105,106 or Tresyl-PEG acrylamide co-vinyl amine. 122 Although of interest these techniques were not further developed probably because of concerns about the toxicity of acrylamide monomers.…”

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confidence: 99%

Progress technology in microencapsulation methods for cell therapy

Rabanel

Banquy

Zouaoui

et al. 2009

Biotechnology Progress

122

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Cell encapsulation in microcapsules allows the in situ delivery of secreted proteins to treat different pathological conditions. Spherical microcapsules offer optimal surface-to-volume ratio for protein and nutrient diffusion, and thus, cell viability. This technology permits cell survival along with protein secretion activity upon appropriate host stimuli without the deleterious effects of immunosuppressant drugs. Microcapsules can be classified in 3 categories: matrix-core/shell microcapsules, liquid-core/shell microcapsules, and cells-core/shell microcapsules (or conformal coating). Many preparation techniques using natural or synthetic polymers as well as inorganic compounds have been reported. Matrix-core/shell microcapsules in which cells are hydrogel-embedded, exemplified by alginates capsule, is by far the most studied method. Numerous refinement of the technique have been proposed over the years such as better material characterization and purification, improvements in microbead generation methods, and new microbeads coating techniques. Other approaches, based on liquid-core capsules showed improved protein production and increased cell survival. But aside those more traditional techniques, new techniques are emerging in response to shortcomings of existing methods. More recently, direct cell aggregate coating have been proposed to minimize membrane thickness and implants size. Microcapsule performances are largely dictated by the physicochemical properties of the materials and the preparation techniques employed. Despite numerous promising pre-clinical results, at the present time each methods proposed need further improvements before reaching the clinical phase.

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Immobilization of enzymes by multilayer microcapsules

Pommersheim

Schrezenmeir

Vogt

1994

Macro Chemistry & Physics

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A method is described to obtain a new type of microcapsules for immobilization of enzymes or living cells. The wall of these capsules consists of several layers of poly(ethy1eneimine) and poly(acry1ic acid). The idea is that diverging properties of the whole assembly can be better controlled when the membrane is built up by several consecutive steps, each being optimized with respect to a special property, for example, permeability or mechanical strength. The encapsulation of acidic phosphatase and the cleavage of p-nitrophenyl phosphate was used as a model system. The charged capsules were characterized by their enzymatic activity, as a function of membrane composition (number and sequence of layers) and storing time. The permeability for the substrate and the retaining ability for the enzyme were also measured.

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“Inverted Microcarriers” for Cell Cultures Made by Polymerization of Shells around Agarose Microspheres in a Non-Cytotoxic Procedure

Cited by 5 publications

References 10 publications

Tunable Volumetric Density and Porous Structure of Spherical Poly-ε-caprolactone Microcarriers, as Applied in Human Mesenchymal Stem Cell Expansion

Tunable Volumetric Density and Porous Structure of Spherical Poly-ε-caprolactone Microcarriers, as Applied in Human Mesenchymal Stem Cell Expansion

Progress technology in microencapsulation methods for cell therapy

Immobilization of enzymes by multilayer microcapsules

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