Invertebrates living in polluted environments are potential source of novel anticancer agents

Soopramanien, Morhanavallee; Mungroo, Mohammad Ridwane; Sagathevan, Kuppusamy; Khan, Naveed Ahmed; Siddiqui, Ruqaiyyah

doi:10.35333/jrp.2019.72

Cited by 8 publications

(19 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To date, there have been no studies conducted to elucidate the anticancer activity of frog and scorpion lysates and sera. Nonetheless, several studies have been accomplished in our laboratory addressing our hypothesis that: "animals living in polluted environments may have anticancer activities" (Jeyamogan et al, 2019;Jeyamogan et al, 2020, Siddiqui et al, 2017Soopramanien et al, 2019). These studies have shown that animals such as crocodiles reveal potent anti-cancer effects.…”

Section: Discussionmentioning

confidence: 99%

“…Growth inhibition assays were accomplished to elucidate the growth inhibitory effect of organ lysates and sera on HeLa, MCF-7, PC3, and HaCaT cells, as previously detailed (Jeyamogan et al, 2019;Soopramanien et al, 2019). Cells were grown at 37 o C in a 5% CO 2 with 95% humidity, until a monolayer of up to 50% confluency was formed.…”

Section: Growth Inhibition Assaysmentioning

confidence: 99%

“…The cells were incubated in similar conditions: 37 o C in a 5% CO 2 with 95% humidity, until the untreated cells became 100% confluent. The treated and untreated cells were then trypsinized with 2.5% trypsin for 15 min and subjected to trypan blue exclusion assay using a haemocytometer to determine cell viability (Jeyamogan et al, 2019;Soopramanien et al, 2019). The growth inhibition effects were established by comparing the number of viable cells of the untreated cells (control) and treated cells.…”

Section: Several Lysates From Uropygi (Vinegaroon Scorpion) Exhibitedmentioning

confidence: 99%

“…To assess the cytotoxic effects of the organ lysate and sera against cell lines, lactate dehydrogenase (LDH) assay was conducted as described previously (Jeyamogan et al, 2019;Siddiqui et al, 2017;Soopramanien et al, 2019). For this, confluent monolayers of cancer and normal cells were grown in 96-well plates for 24 h at 37oC in a 5% CO 2 with 95% humidity.…”

Section: Cytotoxicity Assaysmentioning

confidence: 99%

See 3 more Smart Citations

Scorpion and Frog Organ Lysates are Potential Source of Antitumour Activity

Soopramanien

Khan

Abdalla

et al. 2020

Asian Pac J Cancer Prev

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Growth Inhibition Assaysmentioning

confidence: 99%

Section: Several Lysates From Uropygi (Vinegaroon Scorpion) Exhibitedmentioning

confidence: 99%

Section: Cytotoxicity Assaysmentioning

confidence: 99%

See 2 more Smart Citations

Scorpion and Frog Organ Lysates are Potential Source of Antitumour Activity

Soopramanien

Khan

Abdalla

et al. 2020

Asian Pac J Cancer Prev

Self Cite

View full text Add to dashboard Cite

show abstract

“…The gut microbes were then isolated from the gut using sterile cotton swabs and dispersed onto nutrient agar and blood agar plates. The agar plates were then kept overnight at 37 °C [ 13 , 19 ].…”

Section: Methodsmentioning

confidence: 99%

Heterometrus spinifer: An Untapped Source of Anti-Tumor Molecules

Soopramanien

Khan

Ghimire

et al. 2020

Biology

Self Cite

View full text Add to dashboard Cite

Despite intensive research, cancer incidence and mortality continue to rise. Consequently, the necessity to develop effective anti-cancer therapy is apparent. We have recently shown that the gut bacteria of animals living in polluted environments, such as crocodiles, are a potential source of novel anti-tumor molecules. To extend this work to other resilient species, we investigated the anti-tumor effects of gut bacteria of Heterometrus spinifer (a scorpion). Bacteria from the feces and gut were isolated, identified and evaluated for their anti-tumor effects. Bacterial-conditioned media was prepared in Roswell Park Memorial Institute (RPMI) 1640 media, and cytotoxicity and growth inhibitory properties were examined against cervical (HeLa) cancer cells. Liquid chromatography–mass spectrometry (LC-MS) was conducted to establish the identity of the molecules. Eighteen bacteria species from the gut (HSG01-18) and ten bacteria species from feces (HSF01-10) were tested for anti-tumor effects. Bacterial-conditioned media from scorpion gut and feces exhibited significant growth inhibitory effects against HeLa cells of 66.9% and 83.8%, respectively. Microscopic analysis of cancer cells treated with conditioned media HSG12 and HSG16 revealed apoptosis-like effects. HSG12 was identified as Pseudomonas aeruginosa and HSG16 was identified as Bacillus subtilis. Both conditioned media exhibited 100% growth inhibitory effects versus a selection of cancer cells, comprising cervical, breast and prostate cancer cells. LC–MS indicated the presence of 72 and 38 compounds, detected from HSG12 and HSG16, respectively. Out of these compounds, 47 were successfully identified while the remainder were unidentified and are possibly novel. This study suggests that the fecal and gut microbiota of scorpions might possess molecules with anti-cancer properties, however, further intensive research is needed to assess these expectations.

show abstract