Invertebrate immune diversity

Ghosh, Julie; Lun, Cheng Man; Majeske, Audrey J.; Sacchi, Sandro; Schrankel, Catherine S.; Smith, L. Courtney

doi:10.1016/j.dci.2010.12.009

Cited by 157 publications

(85 citation statements)

References 157 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Hence, we speculate that PmVRP15 is not universally associated with WSSV susceptibility in other populations besides the Thai population. Furthermore, there are extensive studies indicating that WSSV resistance and WSSV susceptibility in penaeid shrimps is synergic immune response regulated by many genes (Ghosh et al, 2011), such as anti-lipopolysaccharide factor (ALF), which plays a role in defense against WSSV (Liu et al, 2014;Wang et al ,2013), Penaeus monodon antiviral (PmAV), which presumably acts as an intermediate factor during antiviral reaction (Luo et al, 2003), and other antimicrobial peptides like penaeidin class 5 that protect shrimps at early infection of WSSV (Jeswin et al, 2013;Wang et al, 2013). Perhaps WSSV susceptibility Al Haq et al in G 8 and G 8iA can be marked with other considerable immune-related gene expression rather than PmVRP15.…”

Section: Fig 2 Cumulative Mortality Of P Monodon Fabricius In G 8 Amentioning

confidence: 99%

Limited evidence for white spot syndrome virus susceptibility associated with expression of PmVRP15 in local population of giant tiger shrimp (Penaeus monodon)

et al. 2017

View full text Add to dashboard Cite

White spot syndrome virus (WSSV) is a devastating viral disease in shrimp aquaculture. Infection of WSSV in penaeid shrimps affects immune defense and changes gene expression. PmVRP15 has been reported as a part of the WSSV propagation pathway that is highly up-regulated in hemocytes at the acute phase of WSSV infection. This study analyzed the expression of PmVRP15 in local populations of giant tiger shrimp (Penaeus monodon) to be associated with susceptibility to WSSV. Tested populations consisted of an inbreeding population (G 8 ) and outbreeding population (G 8iA ) from Jepara, Indonesia. Susceptibility was determined by cumulative mortality, median lethal time (LT 50 ), and severity of infection at time of death. Though all populations were susceptible to WSSV, the first mortality in G 8 occurred at 18 hours post-infection (hpi) with mild infection, while first mortality of G 8iA occurred at 30 hpi with severe infection. The LT 50 of G 8 was significantly lower than that of G 8iA , indicating that G 8iA was less susceptible to WSSV than G 8 . Relative PmVRP15 transcripts of G 8iA were insignificantly down-regulated, whereas relative PmVRP15 transcripts of G 8 were insignificantly upregulated. Although it's still not conclusive, the results of this study suggest that PmVRP15 has weak potential as a WSSV susceptibility marker in G 8 and G 8iA broodstock selection.

show abstract

Section: Fig 2 Cumulative Mortality Of P Monodon Fabricius In G 8 Amentioning

confidence: 99%

Limited evidence for white spot syndrome virus susceptibility associated with expression of PmVRP15 in local population of giant tiger shrimp (Penaeus monodon)

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Invertebrates do not possess such an immune system, and these animals, therefore, rely on innate immune mechanisms for internal defense to protect themselves against various infectious agents (Rowley and Powell, 2007;Ghosh et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Physico-Chemical Characterization of a naturally occurring Hemagglutinin in the Serum of Sand Lobster, Thenus orientalis with Affinity for N-Acetylated Aminosugars

Vaishnavi¹,

Mullainadhan²

2017

Int.J.Curr.Microbiol.App.Sci

View full text Add to dashboard Cite

“…flanked by introns in the same phase), meaning that exon rearrangement of the resulting transcript could occur without disruption to the translational reading frame. Alternative splicing is a commonly observed form of immune and allorecognition molecule diversification (reviewed by Ghosh et al 2011), occurring for example in the allorecognition molecules of the ascidian Botryllus schlosseri (Nyholm et al 2006;McKitrick et al 2011) and the colonial hydroid Hydractinia symbiolongicarpus (Rosa et al 2010). However, searches for alternative exon usage across seventeen A. queenslandica transcriptomes and with the polymerase chain reaction failed to reveal convincing evidence that this is a widespread mechanism of AF diversification.…”

Section: Genomic Architecture and Splicing Of The A Queenslandica Afsmentioning

confidence: 99%

“…For example, fester and uncle fester, of the Botryllus schlosseri histocompatibility system, are both alternatively spliced (Nyholm et al 2006;McKitrick et al 2011), as is the Hydractinia symbiolongicarpus allorecognition gene alr1 (Rosa et al 2010). Notably, these and other immune-related genes (Ghosh et al 2011 . This between-individual variance was not revealed until after sequencing was complete;…”

Section: Transcriptome and Qpcr Data Do Not Reveal Dynamic Expressionmentioning

confidence: 99%

“…Clustering of allorecognition genes facilitates the transfer of sequence information between regions within an immune locus, which may be executed in a number of ways including gene conversion, recombination and unequal crossing over, alternative splicing and gene inversion (Graham 1995;Ghosh et al 2011). The clustering of related allorecognition genes may increase the efficiency and precision of co-regulated gene expression if required (Blumenthal 1998), as has been observed in suites of non-allorecognition genes from diverse taxa, such as zebrafish (Ng et al 2009), Caenorhabditis elegans (Spieth et al 1993), Saccharomyces cerevisiae (Zhang and Smith 1998) and D. melanogaster (Spellman and Rubin 2002).…”

Section: ) Includingmentioning

confidence: 99%

See 1 more Smart Citation

Self-nonself recognition: genomic and transcriptomic insights from the sponge aggregation factors

Grice¹

View full text Add to dashboard Cite

The multicellular condition cannot be maintained without safeguards protecting the integrity of the individual. Tissue contact and fusion with other conspecific individuals may threaten this integrity, as genetically non-identical cells may shirk their somatic duties and gain disproportionate access to the germ line. As sessile invertebrates that commonly inhabit crowded benthic environments, sponges are particularly reliant on a molecular self-nonself defense system in order to resist loss of habitat space, chimerism and possible germ line parasitism by neighbouring conspecific sponges. Sponge allorecognition appears to be, at least in part, under the control of extracellular proteoglycans called aggregation factors (AFs), which were first discovered based on their role in the species-specific reaggregation of dissociated sponge cells. Although the AFs have been extensively studied for over fifty years, the majority of this work has involved biochemical, rather than genetic approaches, and has focussed on the role of the glycan subunits associated with the AFs. In the present work, I investigate the genetic properties underlying the AF protein backbone, to better understand the functions and evolution of these putative allorecognition molecules.Using newly-available genomic and transcriptomic data, I surveyed the phylum Porifera for novel putative AF sequences, to explore the evolutionary origins of this gene family. I conclude that the AFs are a demosponge and hexactinellid-specific innovation. I then performed an in-depth characterisation of the six AF genes from the model demosponge species, Amphimedon queenslandica. The six genes display a highly modular intron/exon organisation. However, as expected of putative allorecognition genes, the AFs are greatly diversified between individuals, with nucleotide polymorphism (and possible positive selection) and intron retention events distributed across the six genes. The AFs are very highly expressed across sponge development and in response to alloimmune challenge, and undergo a particular spike in gene expression levels after the onset of sponge metamorphosis. The AF genes also exhibit expression patterns across development that are significantly correlated with those of other, developmentally important genes with roles in various cell signalling pathways. I conclude that the AFs play a novel developmental role, in addition to their putative allorecognition capabilities.iii S e l f -N o N S e l f R e c o g N i t i o N : S p o N g e ag g R e g at i o N f a c t o R S Declaration by authorThis thesis is composed of my original work, and contains no material previously published or written by another person except where due reference has been made in the text. I have clearly stated the contribution by others to jointly-authored works that I have included in my thesis.I have clearly stated the contribution of others to my thesis as a whole, including statistical assistance, survey design, data analysis, significant technical procedures, professional editorial ...

show abstract

Invertebrate immune diversity

Cited by 157 publications

References 157 publications

Limited evidence for white spot syndrome virus susceptibility associated with expression of PmVRP15 in local population of giant tiger shrimp (Penaeus monodon)

Limited evidence for white spot syndrome virus susceptibility associated with expression of PmVRP15 in local population of giant tiger shrimp (Penaeus monodon)

Physico-Chemical Characterization of a naturally occurring Hemagglutinin in the Serum of Sand Lobster, Thenus orientalis with Affinity for N-Acetylated Aminosugars

Self-nonself recognition: genomic and transcriptomic insights from the sponge aggregation factors

Contact Info

Product

Resources

About