Invertase production of common storage moulds in food and feed grains as a possibility for rapid detection of aspergillus flavus group and Aspergillus fumigatus

Mátrai, T.; Mayer, Susan E.; Kókai, Susan; Salamon, Irene

doi:10.1016/s0168-1605(00)00360-3

Cited by 14 publications

(5 citation statements)

References 5 publications

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“…The prior studies were carried out to assess invertase production using different types of microorganisms such as Zymomonas mobilis (V'asquez- Bahena et al, 2006), Saccharomyces cerevisiae (Tanaka et al, 2000), Aspergillus fumigatus and A. flavus group (Matrai et al, 2000), A. niger (Ge and Zhang, 2005;Rubio and Maldonado, 1995;Rubio and Navarro, 2006), A. nidulans (Vainstein and Pedery, 1990), A. japonicus (Chen and Liu, 1996) and Penicillium sp. (Nakamura et al, 1997).…”

Section: Introductionmentioning

confidence: 99%

Effects of extremely low magnetic field on the production of invertase by Rhodotorula glutinis

et al. 2010

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Invertase is an important enzyme used in many fields especially in food industry to produce fructose syrups. The current study focused on increasing invertase production by exposing Rhodotorula glutinis to extremely low magnetic fields (ELMF; 0 and 7 mT). For this purpose, the microorganism was allowed to grow in normal magnetic field and ELMF for 72 hours at the same temperature (24 ± 2°C). The fermentation was carried out in submerged culture for 120 hours. The results showed that invertase production is strongly dependent on the growth conditions of the microorganism. Both of the different magnetic fields applied to R. glutinis increased invertase production ranged from 48%-67% when compared with the control. On the other hand, ELMF treatment increased biomass formation about 14%-28% when compared with the control. As a result, magnetic field treatment could effectively be used in the production of invertase by R. glutinis.

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Section: Introductionmentioning

confidence: 99%

Effects of extremely low magnetic field on the production of invertase by Rhodotorula glutinis

et al. 2010

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“…Aspergillus niger is also thought to produce invertase [2]. The sugar industry is comparatively a minor user of enzymes but provides a few historically signifi cant and instructive examples of enzyme technology [3]. The hydrolysis (inversion) of sucrose, completely or partially into glucose and fructose provides sweet syrups that are more stable than pure sucrose syrups.…”

Section: Introductionmentioning

confidence: 99%

Production and partial purification of invertase using Cympopogan caecius leaf powder as substrate

Shankar¹,

Sathyavani

Niket³

2010

Indian J Microbiol

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The present study investigates the effi ciency of Aspergillus niger to produce invertase, an industrially important enzyme by using powdered stem of Cympopogan caecius (Lemon grass) as sole substrate and sole carbon source for the microorganism. The molecular weight of invertase was estimated to be 66-70 kDa by sodium do decyl sulphate poly acrylamide gel electrophoresis (SDS PAGE). The production of the enzyme was studied at different pH scales ranging from pH 4.0 to 7.0 at a constant temperature of 30°C and 2% substrate concentration. The maximum production of invertase (specifi c activity -0.0516 μk/mg protein) was obtained at pH 5.5 at 30°C temperature, and incubation for 48 h. The activity was found to be stable at pH 5.5 for 30 min. The enzyme was found to be stable in the temperature range of 20-55°C. The effect of divalent metal ions Cu 2+ , Fe 2+ , Co 2+ on the activity of the enzyme invertase showed that these ions affected the activity by a certain factor. The study can be further industrially exploited in a country-like India where lemon grass is found in plenty and can be used as substrate for enzyme production. Moreover, the preparation of the substrate is also a simple process.

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“…특히 조류 중에는 특정 성분이 고함량 존재 하는 종들이 많고, 리그닌이 거의 없기 때문에 리그노셀 룰로오스계 바이오매스(리그닌 15~30% 함유)보다 값싼 공 정개발이 가능하다. 그리하여 최근 미세조류 또는 해양 거 대조류를 활용하여 바이오연료 또는 바이오화학소재 생산 을 위한 많은 연구 (Adams et al, 2009;Choi et al, 2010;Ge et al, 2011;Harun et al, 2010;Isa et al, 2009;John et al, 2011;Lee et al, 2009;Mata et al, 2010;Nguyen et al, 2012;Scott et al, 2010;Ueno et al, 1998) (Alvira et al, 2010;Daroch et al, 2013 (Matrai et al, 2000;Nguyen et al, 2012). Table 4.…”

Section: 서 론unclassified

Hydrolysis Methods for the Efficient Manufacture of Sugar Solutions from the Freshwater Alga Water-net (Hydrodictyon reticulatum)

Kim¹,

Kim²,

Ko³

et al. 2013

Weed & Turfgrass Science

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To explore hydrolysis methods for the efficient manufacture of sugar solutions from the freshwater alga Water-net (Hydrodictyon reticulatum, HR), acid hydrolysis, enzymatic hydrolysis, and combined hydrolysis (acid followed by enzymatic hydrolysis) were investigated. In the one-step acid hydrolysis, the reaction of 8% solids content using 2% sulfuric acid at 120 o C for 1 hour was desirable. In this case, glucose 27.44 g 100 g DM-1 could be obtained from the HR-d13 samples. In the two-step acid hydrolysis, the primary hydrolysis (HR powder : 72% sulfuric acid = 1 g : 1.5 mL) was carried out for 1 hour at 60 o C, and then the secondary hydrolysis was done for 1 hour at 120 o C after addition of distilled water 23.5 mL. In this case, glucose 35.11 g/100 g DM could be obtained from the HR-d13 samples. In the combined hydrolysis, 25% solids content using 2% hydrochloric acid were reacted for 1 hour at 120 o C, and then citrate buffer and hydrolysis enzyme complexes (E1 1.0 mL+E2 0.2 mL g-1 dried matter) were added and reacted for 1-2 days at 50 o C. In this case, glucose 33.5 g 100 g DM-1 could be obtained from the HR-d23+26 samples. In conclusion, combined hydrolysis was likely to be more useful saccharification method of HR biomass at a practical level, considering the glucose productivity, generation of fermentation-inhibiting substances (hydroxyl methyl furfural, furfural), and limited use of strong acid.

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Invertase production of common storage moulds in food and feed grains as a possibility for rapid detection of aspergillus flavus group and Aspergillus fumigatus

Cited by 14 publications

References 5 publications

Effects of extremely low magnetic field on the production of invertase by Rhodotorula glutinis

Effects of extremely low magnetic field on the production of invertase by Rhodotorula glutinis

Production and partial purification of invertase using Cympopogan caecius leaf powder as substrate

Hydrolysis Methods for the Efficient Manufacture of Sugar Solutions from the Freshwater Alga Water-net (Hydrodictyon reticulatum)

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