Invertase and its applications – A brief review

Kulshrestha, Samarth; Tyagi, Prasidhi; Sindhi, Vinita; Yadavilli, Kameshwar Sharma

doi:10.1016/j.jopr.2013.07.014

Cited by 88 publications

(95 citation statements)

References 15 publications

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“…However, the combination of D-glucose and a terminal ␤-linked D-fructose, such as sucrose or raffinose, was involved in the structures of these trisaccharides, which induced biofilm formation. In the established consensus, carbohydrates, which possess a terminal ␤-linked D-fructose unit, are susceptible to the action of invertase (37). Invertase is an enzyme for the conversion of sucrose to glucose and fructose, and, like fructosyltransferase, it is important for the utilization of fructose (37,38).…”

Section: Discussionmentioning

confidence: 99%

“…In the established consensus, carbohydrates, which possess a terminal ␤-linked D-fructose unit, are susceptible to the action of invertase (37). Invertase is an enzyme for the conversion of sucrose to glucose and fructose, and, like fructosyltransferase, it is important for the utilization of fructose (37,38). However, melezitose, in lacking a terminal ␤-linked D-fructose unit, is insusceptible to invertase.…”

Section: Discussionmentioning

confidence: 99%

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Raffinose Induces Biofilm Formation by Streptococcus mutans in Low Concentrations of Sucrose by Increasing Production of Extracellular DNA and Fructan

Nagasawa

Sato

Senpuku

2017

Appl Environ Microbiol

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Streptococcus mutans is the primary etiological agent of dental caries and causes tooth decay by forming a firmly attached biofilm on tooth surfaces. Biofilm formation is induced by the presence of sucrose, which is a substrate for the synthesis of extracellular polysaccharides but not in the presence of oligosaccharides. Nonetheless, in this study, we found that raffinose, which is an oligosaccharide with an intestinal regulatory function and antiallergic effect, induced biofilm formation by S. mutans in a mixed culture with sucrose, which was at concentrations less than those required to induce biofilm formation directly. We analyzed the possible mechanism behind the small requirement for sucrose for biofilm formation in the presence of raffinose. Our results suggested that sucrose contributed to an increase in bacterial cell surface hydrophobicity and biofilm formation. Next, we examined how the effects of raffinose interacted with the effects of sucrose for biofilm formation. We showed that the presence of raffinose induced fructan synthesis by fructosyltransferase and aggregated extracellular DNA (eDNA, which is probably genomic DNA released from dead cells) into the biofilm. eDNA seemed to be important for biofilm formation, because the degradation of DNA by DNase I resulted in a significant reduction in biofilm formation. When assessing the role of fructan in biofilm formation, we found that fructan enhanced eDNA-dependent cell aggregation. Therefore, our results show that raffinose and sucrose have cooperative effects and that this induction of biofilm formation depends on supportive elements that mainly consist of eDNA and fructan.IMPORTANCE The sucrose-dependent mechanism of biofilm formation in Streptococcus mutans has been studied extensively. Nonetheless, the effects of carbohydrates other than sucrose are inadequately understood. Our findings concerning raffinose advance the understanding of the mechanism underlying the joint effects of sucrose and other carbohydrates on biofilm formation. Since raffinose has been reported to have positive effects on enterobacterial flora, research on the effects of raffinose on the oral flora are required prior to its use as a beneficial sugar for human health. Here, we showed that raffinose induced biofilm formation by S. mutans in low concentrations of sucrose. The induction of biofilm formation generally generates negative effects on the oral flora. Therefore, we believe that this finding will aid in the development of more effective oral care techniques to maintain oral flora health.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Raffinose Induces Biofilm Formation by Streptococcus mutans in Low Concentrations of Sucrose by Increasing Production of Extracellular DNA and Fructan

Nagasawa

Sato

Senpuku

2017

Appl Environ Microbiol

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“…Due to both the extensive data available in the literature [28][29][30][31][32][33] and commercial relevance [34], the biocatalytic system used on the present work was the hydrolysis of sucrose catalyzed by Saccharomyces cerevisiae invertase yielding an equimolar mixture of glucose and fructose (invert sugars).…”

Section: Introductionmentioning

confidence: 99%

Sucrose Hydrolysis in a Bespoke Capillary Wall-Coated Microreactor

2017

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Abstract:Microscale technology has been increasingly used in chemical synthesis up to production scale, but in biocatalysis the implementation has been proceeding at a slower pace. In this work, the design of a low cost and versatile continuous flow enzyme microreactor is described that illustrates the potential of microfluidic reactors for both the development and characterization of biocatalytic processes. The core structure of the developed reactor consists of an array of capillaries with 450 µm of inner diameter with their inner surface functionalized with (3-aminopropyl)triethoxysilane (APTES) and glutaraldehyde where Saccharomyces cerevisiae invertase was covalently bound. The production of invert sugar syrup through enzymatic sucrose hydrolysis was used as model system. Once the microreactor assembly reproducibility and the immobilized enzyme behavior were established, the evaluation of the immobilized enzyme kinetic parameters was carried out at flow rates ranging from 20.8 to 219.0 µL·min −1 and substrate concentrations within 2.0%-10.0% (w/v). Despite the impact of immobilization on the kinetic parameters, viz. K m(app) was increased two fold and K cat showed a 14-fold decrease when compared to solution phase invertase, the immobilization proved highly robust. For a mean residence time of 48.8 min, full conversion of 5.0% (w/v) sucrose was observed over 20 days.

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“…Thus, use of the transgenic potato plants with the yeast invertase gene could be found some application in medical, biological and pharmaceutical engineering [2]. …”

Section: Discussionmentioning

confidence: 99%

“…The production of non-crystallizable sugar syrup from sucrose is one of the major applications of invertase. Invertase being a powerful anti-microbial agent and an antioxidant aids in the prevention of bacterial infestations and gut fermentation due to oxidation [2]. In higher plants an invertases has several isoforms: 1) alkaline/neutral invertases are localized in the chloroplasts, cytoplasm, and mitochondria; 2) acid invertases are localized in the apoplast (cell-wall invertase) and vacuole [3].…”

Section: Introductionmentioning

confidence: 99%

Transformation of the Solanum tuberosum with Saccharomyces cerevisiae Gene Suc2 Encoding Cell-Wall Invertase Influences on Sugars Distribution in Plants

Deryabin¹,

Trunova²

2017

Foc Sci

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Introduction: Invertase is the key enzyme of carbohydrate metabolism, which catalyzes the sucrose hydrolysis. In this study, we investigated a functional role of the apoplastic invertase (cell-wall invertase) on sugars distribution in vegetative organs and cell compartments of potato plants in vitro. Methods: Our study was carried out with potato (S. tuberosum L., cv. Désirée) plants and the line which expressed the suc2 gene of S. cerevisiae under control of the tuberspecific patatin B33-promoter of class I with an N-end-connected potato proteinase II inhibitor signal peptide, which provides apoplastic localization of yeast invertase. The suc2 gene expression were shown using RT-PCR. The results of Ds-Na-PAGE of apoplast proteins from leaves and MALDI-TOF MS analysis indicate the presence of the yeast invertase in the apoplastic space of the transgenic potato plants. The content of fructose and sucrose was determined according to Roe. The glucose content was determined by the glucose oxidase method. Results: The integrated target suc2 gene encodes the invertase of yeast with an N-endconnected potato proteinase II inhibitor signal peptide, which provides apoplastic localization of foreign invertase. Conclusions: Transformation of potato plants resulted in accumulation of fructose in the apoplast, sucrose and glucose in the leaves, and especially, glucose in roots and microtubers. It is indicative of regulatory function of cell-wall invertase that could be found some application in medical, biological and pharmaceutical engineering.

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Invertase and its applications – A brief review

Cited by 88 publications

References 15 publications

Raffinose Induces Biofilm Formation by Streptococcus mutans in Low Concentrations of Sucrose by Increasing Production of Extracellular DNA and Fructan

Raffinose Induces Biofilm Formation by Streptococcus mutans in Low Concentrations of Sucrose by Increasing Production of Extracellular DNA and Fructan

Sucrose Hydrolysis in a Bespoke Capillary Wall-Coated Microreactor

Transformation of the Solanum tuberosum with Saccharomyces cerevisiae Gene Suc2 Encoding Cell-Wall Invertase Influences on Sugars Distribution in Plants

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