Inverse relationship between whole brain monoamine levels and audiogenic seizure susceptibility in mice: Failure to replicate

Lints, Carlton E.; Willott, James F.; Sze, Paul Y.; Nenja, Leonard H.

doi:10.1016/0091-3057(80)90041-6

Cited by 15 publications

(5 citation statements)

References 10 publications

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“…Unfortunately, the cerebellum samples were not big enough to allow us to measure t-methylhistamine plus histamine. Our results would appear to contradict our previous hypothesis about CNS histamine suppressing seizures [2,6]. However, clinically and experimentally it has been shown that the cerebellum has an influence, both excitatory and inhibitory, on seizures [7,8].…”

Section: Resultscontrasting

confidence: 56%

“…Susceptibility of the DBA/2 mouse to audiogenic seizures is age-related, beginning as soon as they can hear (~day 14) and is virtually lost by day 40 [1]. Controversy exists over the relationship between whole brain levels of 5-HT and noradrenaline and the audiogenic seizure susceptibility in the DBA/2 mouse [2]. Alterations in CNS histamine levels and function have been associated with epileptic seizures in rodents [3] and to date investigations into CNS histamine levels in this mouse strain have not been published.…”

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confidence: 99%

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3. Histaminergic mechanisms in the CNS Brain histamine turnover and seizure behaviour in two mouse strains: Preliminary findings

Tuomisto¹,

Sturman²,

Freeman³

et al. 2003

Inflammation Research

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Epilepsy-prone strains of animal are often used as models for epilepsy. Susceptibility of the DBA/2 mouse to audiogenic seizures is age-related, beginning as soon as they can hear (~day 14) and is virtually lost by day 40 [1]. Controversy exists over the relationship between whole brain levels of 5-HT and noradrenaline and the audiogenic seizure susceptibility in the DBA/2 mouse [2]. Alterations in CNS histamine levels and function have been associated with epileptic seizures in rodents [3] and to date investigations into CNS histamine levels in this mouse strain have not been published. Thus we have measured levels of histamine and its major metabolite in 4 distinct brain regions in male DBA/2 mice compared with a nonaudiogenic-sensitive mouse strain (BKTO). Materials and methodsBoth audiogenic-sensitive (DBA/2) and non-audiogenic-sensitive (BKTO, Bantin & Kingman, Hull) mice were bred and housed at University of East London. Groups of 10 male mice of both strains, at 14, 21, 28, 35 and 42 days of age were killed by cervical dislocation. Brains were rapidly removed onto ice and dissected into 4 regions: cerebral cortex, hypothalamus, cerebellum and rest (mid-brain + brain stem). Perchloric acid (0.4 M) and EDTA (5 mM) were added to each sample. Samples were sonicated on ice and stored at -70°C. Histamine and t-methylhistamine were determined using an HPLC-fluorimetric method and GCMS [4]. Matched groups of mice were tested for audiogenic seizure susceptibility [5]. Data was analysed by ANOVA and appropriate post-tests. All animal work conformed to national animal legislation. Results and discussionBKTO mice did not exhibit audiogenic susceptibility. Maximal audiogenic susceptibility in the DBA/2 mouse occurred at day 21 and was almost lost 3 weeks later (Fig. 1 A). Our findings on the age-related audiogenic susceptibility of the DBA/2 mice agree with previous reports [1, 5].As assessment for audiogenic seizures might alter CNS histamine and t-methylhistamine levels, these were measured in mice which had not been subjected to any loud noises. Levels of histamine were highest in the hypothalamus (~10 nmol/g) and were similar in both strains with the highest levels being found on day 21 and then declining with age Inflamm. res. 52, Supplement 1 (2003) Fig. 1. Groups of 10 male DBA/2 & BKTO mice at weekly intervals were used to determine (A) mean audiogenic seizure scores by the method in [5] (1 = wild running, 2 = clonus, 3 = tonus, 4 = respiratory arrest. Inter-strain differences (Kruskal Wallis ANOVA) and (B) mean cerebellar histamine levels (pmol/g) + SD using HPLC-fluorimetry [4].

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Section: Resultscontrasting

confidence: 56%

mentioning

confidence: 99%

3. Histaminergic mechanisms in the CNS Brain histamine turnover and seizure behaviour in two mouse strains: Preliminary findings

Tuomisto¹,

Sturman²,

Freeman³

et al. 2003

Inflammation Research

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“…There were no differences in these parameters between the two strains in forebrain, mid-brain, hippocampus and pons-medulla (Jazrawi et al, 1989). The failure to replicate previously reported significant differences between the susceptible (DBA/2J) and nonsusceptible (C57BL/6J) mice in the brain monoamine levels at the age of peak seizure susceptibility (in DBAs) demonstrated the difficulties of the whole issue, which was probably due to different techniques used (Lints et al, 1980). The cordotomy and brain monoamine levels approach proved, as authors claimed , that NE and 5-HT are not responsible for attenuation of audiogenic seizures.…”

Section: Neurochemical Correlates Of Audiogenic Seizure Susceptibilitcontrasting

confidence: 40%

Audiogenic Seizures - Biological Phenomenon and Experimental Model of Human Epilepsies

Полетаева¹,

Федотова²,

Sourina³

et al. 2011

Clinical and Genetic Aspects of Epilepsy

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“…The failure to replicate previously reported significant differences between the susceptible (DBA/2J) and nonsusceptible (C57BL/6J) mice in the brain monoamine levels at the age of peak seizure susceptibility (in DBAs) demonstrated the difficulties of the whole issue, which was probably due to different techniques used (Lints et al, 1980). The cordotomy and brain monoamine levels approach proved, as authors claimed (Willott et al, 1979), that NE and 5-HT are not responsible for attenuation of audiogenic seizures.…”

Section: Neurochemical Correlates Of Audiogenic Seizure Susceptibility and Fit Developmentmentioning

confidence: 92%

Clinical and Genetic Aspects of Epilepsy

Afawi¹

2011

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Inverse relationship between whole brain monoamine levels and audiogenic seizure susceptibility in mice: Failure to replicate

Cited by 15 publications

References 10 publications

3. Histaminergic mechanisms in the CNS Brain histamine turnover and seizure behaviour in two mouse strains: Preliminary findings

3. Histaminergic mechanisms in the CNS Brain histamine turnover and seizure behaviour in two mouse strains: Preliminary findings

Audiogenic Seizures - Biological Phenomenon and Experimental Model of Human Epilepsies

Clinical and Genetic Aspects of Epilepsy

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