Inverse Correlation of miRNA and Cell Cycle-Associated Genes Suggests Influence of miRNA on Benign Thyroid Nodule Tumorigenesis

Ferraz, Carolina; Lorenz, Stephan; Wojtaś, Bartosz; Bornstein, Stefan R.; Paschke, Ralf; Eszlinger, Markus

doi:10.1210/jc.2012-2564

Cited by 22 publications

(13 citation statements)

References 42 publications

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“…Cyclin D1 forms protein complex with CDK4 or CDK6, the activity of which is necessary for cell cycle G1/S transition 25. The upregulation of cyclin D1 expression could accelerate the cell cycle progression and eventually lead to tumor cell proliferation 26, 27. According to the present study, we found that IQUB could positively regulate the expression of cyclin D1 in breast cancer cells.…”

Section: Discussionsupporting

confidence: 59%

Upregulated IQUB promotes cell proliferation and migration via activating Akt/GSK3β/β‐catenin signaling pathway in breast cancer

Zhang

et al. 2018

Cancer Medicine

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Breast cancer was the highest incidence of tumor in women, which seriously threaten women's health. Our previous study found that the expression of IQUB (IQ motif and ubiquitin domain containing) was significantly increased in the development of breast cancer by transcriptome sequencing. However, there were no studies on the mechanism of IQUB in tumorigenesis. Further study showed that IQUB expression was significantly increased in breast cancer, which had a significantly positive correlation with pathological differentiation of breast cancer by tissue microarray analysis. Furthermore, we also discovered that IQUB overexpression could obviously promote the proliferation and migration of MCF‐7 cells and increase the proportion of MCF‐7 cells in S and G2/M phase in vitro study, while knockdown of IQUB caused inhibition of cell proliferation and migration in MDA‐MB‐231 cells and increased the proportion of MDA‐MB‐231 cells in G1 phase. Furthermore, IQUB overexpression or knockdown combined with treatment of Licl or MG‐132 showed that IQUB activated Akt to promote GSK3β phosphorylation, which in turn activated Wnt/β‐catenin signaling pathway in breast cancer cells. Taken together, these results indicated that upregulated IQUB promoted breast cancer cell proliferation and migration via activating Akt/GSK3β/β‐catenin signaling pathway, which played an important part in the tumorigenesis and development of breast cancer.

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Section: Discussionsupporting

confidence: 59%

Upregulated IQUB promotes cell proliferation and migration via activating Akt/GSK3β/β‐catenin signaling pathway in breast cancer

Zhang

et al. 2018

Cancer Medicine

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“…qPCR was done in a 10 μL reaction using the miScript SYBR Green PCR kit (Qiagen, Hilden, Germany) and commercially available miScript Primer Assays (forward primers; Qiagen, Hilden, Germany) and the reference RNU6B in combination with the miScript Universal primer (reverse primer) for the cDNA templates [42], and in combination with a library specific reverse primer for the library DNA templates (see Additional file 2 for qPCR outline and primers). qPCR was performed on a Roche LightCycler 480 according to the kit manufacturer’s instructions.…”

Section: Methodsmentioning

confidence: 99%

Analysis options for high-throughput sequencing in miRNA expression profiling

et al. 2014

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BackgroundRecently high-throughput sequencing (HTS) using next generation sequencing techniques became useful in digital gene expression profiling.Our study introduces analysis options for HTS data based on mapping to miRBase or counting and grouping of identical sequence reads. Those approaches allow a hypothesis free detection of miRNA differential expression.MethodsWe compare our results to microarray and qPCR data from one set of RNA samples. We use Illumina platforms for microarray analysis and miRNA sequencing of 20 samples from benign follicular thyroid adenoma and malignant follicular thyroid carcinoma. Furthermore, we use three strategies for HTS data analysis to evaluate miRNA biomarkers for malignant versus benign follicular thyroid tumors.ResultsHigh correlation of qPCR and HTS data was observed for the proposed analysis methods. However, qPCR is limited in the differential detection of miRNA isoforms. Moreover, we illustrate a much broader dynamic range of HTS compared to microarrays for small RNA studies. Finally, our data confirm hsa-miR-197-3p, hsa-miR-221-3p, hsa-miR-222-3p and both hsa-miR-144-3p and hsa-miR-144-5p as potential follicular thyroid cancer biomarkers.ConclusionsCompared to microarrays HTS provides a global profile of miRNA expression with higher specificity and in more detail. Summarizing of HTS reads as isoform groups (analysis pipeline B) or according to functional criteria (seed analysis pipeline C), which better correlates to results of qPCR are promising new options for HTS analysis. Finally, data opens future miRNA research perspectives for HTS and indicates that qPCR might be limited in validating HTS data in detail.

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“…However, Lin et al found that the overexpression of miR‐130b caused a remarkable suppression of HCC cell aggressiveness by inhibiting IRF1 . In addition, miR‐130b may have an effect on the tumourigenesis of cold and benign thyroid nodules by regulating proliferation and apoptosis and the cell cycle through cyclin D1 . TGF‐β1 acts through miR‐130b to promote integrin α5 expression, resulting in the enhanced migration of clear renal carcinoma cells …”

Section: Discussionmentioning

confidence: 99%

MiR‐130b promotes the progression of oesophageal squamous cell carcinoma by targeting SASH1

Zhu

Peng

et al. 2018

J Cellular Molecular Medi

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MiR‐130b and SAM and SH3 domain containing 1 (SASH1) play an important role in many types of human cancers. The aim of our research was to study their interactions in the process of the proliferation and aggressiveness of oesophageal squamous cell carcinoma (ESCC) cells. Microarray analysis was done to screen the differentially expressed genes in the ESCC tissues. miR‐130b and SASH1 mRNA levels in the ESCC tissues and cells were detected by qRT‐PCR. Dual luciferase reporter system was used to verify the target relationship between miR‐130b and SASH1. The effects of miR‐130b on SASH1 expression were explored by western blot in KYSE30 and TE1 cell lines. CCK‐8 assay, flow cytometry, Transwell, and wound healing assays were conducted to explore the effects of miR‐130b and SASH1 in vitro. In addition, in vivo experiments were conducted to study the roles of miR‐130b and SASH1. miR‐130b was highly expressed, while SASH1 was the opposite in both the ESCC tissues and cells. The expression of SASH1 was inhibited by the direct binding of miR‐130b. The inhibition of miR‐130b reduced the proliferation and aggressiveness of ESCC cells, while it also induced apoptosis and cell cycle arrest in the ESCC cells by suppressing SASH1. The in vivo assay suggested that the overexpression of miR‐130b promoted the growth of ESCC tumours. MiR‐130b was up‐regulated in the ESCC tumour tissues and cells, acting as a tumour promoter. A stimulating effect was demonstrated on ESCC cell growth and aggressiveness by suppressing SASH1, which is an anti‐oncogene.

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Inverse Correlation of miRNA and Cell Cycle-Associated Genes Suggests Influence of miRNA on Benign Thyroid Nodule Tumorigenesis

Abstract: miR-31 and miR-130b may have an effect on tumorigenesis of CBTNs by regulating proliferation and apoptosis and the cell cycle through cyclin D1.

Cited by 22 publications

References 42 publications

Upregulated IQUB promotes cell proliferation and migration via activating Akt/GSK3β/β‐catenin signaling pathway in breast cancer

Upregulated IQUB promotes cell proliferation and migration via activating Akt/GSK3β/β‐catenin signaling pathway in breast cancer

Analysis options for high-throughput sequencing in miRNA expression profiling

MiR‐130b promotes the progression of oesophageal squamous cell carcinoma by targeting SASH1

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