In this paper, a simulated annealing (SA)-based optimisation is carried out for simultaneous estimation of the Reynolds number (Re) and the dimensions of the enclosure (l x , l y ) from the knowledge of centreline velocity field. For demonstrating the retrieval methodology, the required centreline velocity field is first obtained from a forward method using some known values of the unknowns, which are ultimately estimated by the inverse method. SA is used to optimise the objective function represented by the square of the difference between the known field and an arbitrary guessed field (calculated using some guessed value of the unknowns). For studying the sensitivity of the estimated parameters, the effect of random errors has been investigated and the suitability of the SA has been checked for different initial guesses. The algorithm reported in the present work is useful in estimating the above unknowns (Re, l x , l y ) for a given velocity field.