Inverse agonism at the Na/K‐ATPase receptor reverses EMT in prostate cancer cells

Banerjee, Moumita; Li, Zhichuan; Gao, Yingnyu; Lai, Fangfang; Huang, Minqi; Zhang, Zhongbing; Cai, Liquan; Sanabria, Juan; Gao, Tianyan; Xie, Zijian; Pierre, Sandrine V.

doi:10.1002/pros.24144

Cited by 5 publications

(5 citation statements)

References 49 publications

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“…One important step in the process is the infiltration of tumor cells into the circulatory system through the basement membrane, and EMT plays a key role in this metastasis cascade reaction. EMT causes tumor cells to lose epithelial-like polarity and acquire mesenchymal characteristics, thereby increasing the metastatic and invasive potential of the cells [ 16 ]. EMT promotes tumor metastasis by upregulating or downregulating the expression of epithelial markers such as MMP-2, MMP-9, VEGF, vimentin, E-cad, and N-cad [ 17 ].…”

Section: Discussionmentioning

confidence: 99%

circSPECC1 Promotes Proliferation and Migration of LNCaP Prostate Cancer Cells by Affecting Their Epithelial-Mesenchymal Transition

Xie

Shen

Han

et al. 2023

Journal of Immunology Research

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Objective. To determine the effects of circSPECC1 (hsa_circ_0000745) on the proliferation and migration of LNCaP prostate cancer cells and to explore the potential molecular mechanism. Methods. Stable circSPECC1 shRNA-expressing and circSPECC1-overexpressing LNCaP cell lines were constructed, and relative gene expression levels were determined by RT-PCR. MTT and clonogenic assays were used to assess proliferative ability while a scratch test was used to analyze cell migration. Western blotting was used to determine protein expression levels. The effects of circSPECC1 on the proliferation of LNCaP prostate cancer cells were observed in vivo. Results. circSPECC1 was found to be derived from the SPECC1 (sperm antigen with calponin homology and coiled-coil domains 1) parent gene and to form a loop. Overexpression of circSPECC1 promoted the proliferation and migration of the LNCaP cells, whereas decreased expression of circSPECC1 inhibited these properties. Overexpression of circSPECC1 promoted the expression of MMP-2, MMP-9, VEGF, vimentin, and N-cad but downregulated the expression of E-cad. Decreased expression of circSPECC1 inhibited the expression of MMP-2, MMP-9, VEGF, vimentin, and N-cad but increased the expression of E-cad. Conclusion. circSPECC1 promotes cell proliferation and migration by affecting the epithelial-mesenchymal transition of LNCaP prostate cancer cells.

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Section: Discussionmentioning

confidence: 99%

circSPECC1 Promotes Proliferation and Migration of LNCaP Prostate Cancer Cells by Affecting Their Epithelial-Mesenchymal Transition

Xie

Shen

Han

et al. 2023

Journal of Immunology Research

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“…Studies have shown that it also triggers a metabolic switch from mitochondrial oxidative phosphorylation to aerobic glycolysis (Warburg effect) [103]. Reduced alpha 1 NKA expression and subsequent Src/FAK pathway activation enhances prostate cancer metastatic potential, through decreased E-cadherin and increased c-myc expression [104].…”

Section: P-class Pumpsmentioning

confidence: 99%

The Role οf Ion Channels in the Development and Progression of Prostate Cancer

Sakellakis

Chalkias

2023

Mol Diagn Ther

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Numerous studies have firmly established the role of ion channels in essentially all basic cellular functions. Apart from their role in ion transport, they can form macromolecular complexes with adhesion proteins, and signaling molecules. Ion channels are not only responsible for cellular electrogenesis and excitability, but they also regulate the necessary conditions for tissue homeostasis, such as differentiation, proliferation and apoptosis.Although cancer is not officially classified as a channelopathy, it has been increasingly recognized that ion channel aberrations play an important role in virtually all cancer types.Ion channels can exert pro-tumorigenic activities due to genetic or epigenetic alterations, or as a response to molecular signals, such as growth factors, hormones, etc. Prostate cancer is the second leading cause of cancer-related death in men in the United States. Increasing evidence suggests that ion channels and pumps play a critical role in the regulation of prostate cancer cell proliferation, apoptosis evasion, migration, epithelial-to-mesenchymal transition and angiogenesis. There is also evidence suggesting that ion channels might play a role in treatment failure in prostate cancer. Hence, they represent promising targets for diagnosis, staging and treatment. Here, the role of major types of ion channels involved in the development and progression of prostate cancer were reviewed. Identifying the

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“…For instance, 2D cells are stretched out on a flat substrate, while 3D cells on a natural or synthetic scaffold material maintain normal morphology and multiple contacts with the surrounding cells, and these differences in the morphological spread contribute to the differences in the drug response between the two. Moreover, the difference in the expression and the spatial organization of surface receptors in these two models also affects the response to drugs since the levels of receptors and the binding efficiency of a drug to these receptors is different due to the difference in the structure, localization, and spatial organization of these receptors on cell surfaces [ 93 , 94 ]. In addition, there is a difference in cancer gene expression levels because in 2D cell culture, some genes are differently expressed, which can lead to the different response and, thus, affect the effectiveness of a drug [ 93 , 95 ].…”

Section: Three-dimensional Prostate Cancer Models In Drug Discoverymentioning

confidence: 99%

Let’s Go 3D! New Generation of Models for Evaluating Drug Response and Resistance in Prostate Cancer

Petrić

Sabol

2023

IJMS

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Prostate cancer (PC) is the third most frequently diagnosed cancer worldwide and the second most frequent in men. Several risk factors can contribute to the development of PC, and those include age, family history, and specific genetic mutations. So far, drug testing in PC, as well as in cancer research in general, has been performed on 2D cell cultures. This is mainly because of the vast benefits these models provide, including simplicity and cost effectiveness. However, it is now known that these models are exposed to much higher stiffness; lose physiological extracellular matrix on artificial plastic surfaces; and show changes in differentiation, polarization, and cell–cell communication. This leads to the loss of crucial cellular signaling pathways and changes in cell responses to stimuli when compared to in vivo conditions. Here, we emphasize the importance of a diverse collection of 3D PC models and their benefits over 2D models in drug discovery and screening from the studies done so far, outlining their benefits and limitations. We highlight the differences between the diverse types of 3D models, with the focus on tumor–stroma interactions, cell populations, and extracellular matrix composition, and we summarize various standard and novel therapies tested on 3D models of PC for the purpose of raising awareness of the possibilities for a personalized approach in PC therapy.

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Inverse agonism at the Na/K‐ATPase receptor reverses EMT in prostate cancer cells

Cited by 5 publications

References 49 publications

circSPECC1 Promotes Proliferation and Migration of LNCaP Prostate Cancer Cells by Affecting Their Epithelial-Mesenchymal Transition

circSPECC1 Promotes Proliferation and Migration of LNCaP Prostate Cancer Cells by Affecting Their Epithelial-Mesenchymal Transition

The Role οf Ion Channels in the Development and Progression of Prostate Cancer

Let’s Go 3D! New Generation of Models for Evaluating Drug Response and Resistance in Prostate Cancer

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