Inventing Elvis

Haeussler, Mathias

doi:10.5040/9781350109285

Cited by 3 publications

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several approaches have been developed 1 to reduce the off-target effects 2 of the SpCas9 nuclease of the type II CRISPR system, which is the most commonly used tool for gene editing applications. Besides approaches that increase the length of the recognition sequence of the nuclease [3][4][5][6] or ones that limit its activity in space and/or time [7][8][9][10] , mutations introduced to the sequence of the ribonucleoprotein complex, affecting either the RNA or the protein component, also seem to be an effective way to increase the specificity of editing.…”

Section: Introductionmentioning

confidence: 99%

SuperFi-Cas9 exhibits extremely high fidelity but reduced activity in mammalian cells

Kulcsár

Tálas

Welker

2022

Preprint

View full text Add to dashboard Cite

Several advances have been made to SpCas9, the most widely used CRISPR/Cas genome editing tool, to reduce its unwanted off-target effects. The most promising approach is the development of increased fidelity nuclease (IFN) variants of SpCas9, however, their fidelity has increased at the cost of reduced activity. SuperFi-Cas9 has been developed recently, and it has been described as a next-generation high fidelity SpCas9 variant, free from the drawbacks of the first-generation IFNs. In this study, we characterized the on-target activity and the off-target propensity of SuperFi-Cas9 in mammalian cells comparing it to first-generation IFNs. SuperFi-Cas9 demonstrated strongly reduced activity but exceptionally high fidelity exhibiting features that are in many aspects similar to those of the first-generation variants, such as evo- and HeFSpCas9. When combined with ABE8e, SuperFi-Cas9 produced DNA editing with high activity rate as well as high specificity by reducing both bystander and SpCas9-dependent off-target base editing.

show abstract

Section: Introductionmentioning

confidence: 99%

SuperFi-Cas9 exhibits extremely high fidelity but reduced activity in mammalian cells

Kulcsár

Tálas

Welker

2022

Preprint

View full text Add to dashboard Cite

show abstract

Senses of an ending: Danish reactions to the death of Elvis Presley in 1977

Nygaard

2022

Celebrity Studies

View full text Add to dashboard Cite

Uwolnić „soul”, wracając do Memphis

Szczerbakiewicz

2023

Przegląd Kulturoznawczy

View full text Add to dashboard Cite

The transgressive potential of Elvis Presley’s music and stage persona remains an inadequately explored topic. He was an affective medium which catalysed a moral, racial and social revolution. He was the first beneficiary of emancipatory transformations of sexuality. The narrations and mythologies of rock culture did not problematise Elvis’s art and image transitions – the reasons for his escapes from media stereotypes. The most salient of the medially disjointed images is Elvis’s tenuous identification with the gender role of a male role model imposed upon him. Indeed, rock heroes are to embody the “macho” role. Meanwhile, the most interesting part of Elvis’s musical identity is precisely the ambiguous status of his masculinity in the 1950s and the “non-normativity” of his persona and music in 1960s–70s.

show abstract

Inventing Elvis

Cited by 3 publications

References 0 publications

SuperFi-Cas9 exhibits extremely high fidelity but reduced activity in mammalian cells

SuperFi-Cas9 exhibits extremely high fidelity but reduced activity in mammalian cells

Senses of an ending: Danish reactions to the death of Elvis Presley in 1977

Uwolnić „soul”, wracając do Memphis

Contact Info

Product

Resources

About