Invasiveness of <i>Salmonella typhi</i> Strains in HeLa S3 Monolayer Cells

Yabuuchi, Eiko; Ikedo, Masanari; Ezaki, Takayuki

doi:10.1111/j.1348-0421.1986.tb03050.x

Cited by 32 publications

(20 citation statements)

References 21 publications

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“…K. pneumoniae Ca0438 replicated within intestinal epithelial cells during the first 6 h after the addition of gentamicin and survived for ϳ72 h. One study of S. Typhimurium showed an increased number of intracellular bacteria within 24 h inside MDCK epithelial cells (54). Another study also reported that a Salmonella enterica serovar Typhi clinical isolate increased within 24 h inside HeLa cells (55). A K. pneumoniae UTI isolate in a previous study was reported to replicate inside bladder epithelial cells within 4 h and survived for at least 96 h (24).…”

Section: Discussionmentioning

confidence: 86%

Klebsiella pneumoniae Translocates across the Intestinal Epithelium via Rho GTPase- and Phosphatidylinositol 3-Kinase/Akt-Dependent Cell Invasion

et al. 2015

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bKlebsiella pneumoniae is an important pathogen that causes hospital-acquired septicemia and is associated with the recent emergence of community-acquired pyogenic liver abscess (PLA). Clinical typing suggests that K. pneumoniae infections originate from the gastrointestinal reservoir. However, the underlying mechanism remains unknown. Here, we have sought to determine how K. pneumoniae penetrates the intestinal barrier. We identified that bacteremia and PLA clinical isolates adhered to and invaded intestinal epithelial cells. Internalization of K. pneumoniae in three different human colonic cell lines was visualized by confocal microscopy and three-dimensional (3D) imaging. Using a Transwell system, we demonstrated that these K. pneumoniae isolates translocated across a polarized Caco-2 monolayer. No disruptions of transepithelial electrical resistance and altered distribution of tight junction protein ZO-1 or occludin were observed. Therefore, K. pneumoniae appeared to penetrate the intestinal epithelium via a transcellular pathway. Using specific inhibitors, we characterized the host signaling pathways involved. Inhibition by cytochalasin D and nocodazole suggested that actin and microtubule cytoskeleton were both important for K. pneumoniae invasion. A Rho inhibitor, ML141, LY294002, and an Akt1/2 inhibitor diminished K. pneumoniae invasion in a dose-dependent manner, indicating that Rho family GTPases and phosphatidylinositol 3-kinase (PI3K)/Akt signaling were required. By a mouse model of gastrointestinal colonization, in vivo invasion of K. pneumoniae into colonic epithelial cells was demonstrated. Our results present evidence to describe a possible mechanism of gastrointestinal translocation for K. pneumoniae. Cell invasion by manipulating host machinery provides a pathway for gut-colonized K. pneumoniae cells to penetrate the intestinal barrier and access extraintestinal locations to cause disease.

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Section: Discussionmentioning

confidence: 86%

Klebsiella pneumoniae Translocates across the Intestinal Epithelium via Rho GTPase- and Phosphatidylinositol 3-Kinase/Akt-Dependent Cell Invasion

et al. 2015

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“…Viable bacteria of strain 10007 were found to internalize into monolayered HeLa cells within 10 min after inoculation, and multiplied intracellularly (10). Ultrastructural findings of this phenomenon were also described and illustrated in a following paper (11).…”

Section: Discussionmentioning

confidence: 99%

Cytopathogenic Effect of Salmonella typhi GIFU 10007 on M cells of Murine Ileal Peyer's Patches in Ligated Ileal Loops: An Ultrastructural Study

Kohbata

Yokoyama

Yabuuchi

1986

Microbiology and Immunology

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207

100

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“…The bacterial strains used in this study were S. enterica serovar Typhi GIFU10007 [17], z66 antigen-positive strains of S. enterica serovar Typhi isolated in India (S2) and Indonesia (S4-S8), S. enterica serovar Typhi Ty2, and z66 antigen-negative strains of S. enterica serovar Typhi isolated in Japan (S1 and S3) and Thailand (T1 and T2). E. coli DH5a was used for cloning with the pGEM Ò -T Easy vector (Promega).…”

Section: Bacterial Strains and Plasmidsmentioning

confidence: 99%

Cloning and characterization of the gene encoding the z66 antigen of Salmonella enterica serovar Typhi

Huang

Phung

Dejsirilert

et al. 2004

FEMS Microbiology Letters

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Z66 antigen-positive strains of Salmonella enterica serovar Typhi change flagellin expression in only one direction from the z66 antigen to the d or j antigen, which is different from the phase variation of S. enterica serovar Typhimurium. In the present study, we identified a new flagellin gene in z66 antigen-positive strains of S. enterica serovar Typhi. The genomic structure of the region containing this new flagellin gene was similar to that of fljBA operon of biphasic S. enterica serovars. A fljA-like gene was present downstream of the new flagellin gene. A rho-independent terminator was located between the new flagellin gene and the fljA-like gene. Hin-like gene was not present upstream of the new flagellin gene. We generated a mutant strain of S. enterica serovar Typhi, which carries a deletion of the new flagellin gene. Western blotting revealed that the 51-kDa z66 antigen protein was absent from the population of proteins secreted by the mutant strain. Southern hybridization demonstrated that the z66 antigen-positive strains of S. enterica serovar Typhi carried the new flagellin gene and fliC on two different genomic EcoRI fragments. When z66 antigen-positive strains were incubated with anti-z66 antiserum, the flagellin expression by S. enterica serovar Typhi changed from z66 antigen to j antigen. The new flagellin gene and the fljA-like gene were absent in the strain with altered flagellin expression. These results suggested that the new flagellin gene is a fljB-like gene, which encodes the z66 antigen of S. enterica serovar Typhi, and that deletion of fljBA-like operon may explain why S. enterica serovar Typhi alters the flagellin expression in only one direction from the z66 antigen to the d or j antigen.

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Invasiveness of Salmonella typhi Strains in HeLa S3 Monolayer Cells

Abstract: The internalization and intracellular multiplication, i.e

Cited by 32 publications

References 21 publications

Klebsiella pneumoniae Translocates across the Intestinal Epithelium via Rho GTPase- and Phosphatidylinositol 3-Kinase/Akt-Dependent Cell Invasion

Klebsiella pneumoniae Translocates across the Intestinal Epithelium via Rho GTPase- and Phosphatidylinositol 3-Kinase/Akt-Dependent Cell Invasion

Cytopathogenic Effect of Salmonella typhi GIFU 10007 on M cells of Murine Ileal Peyer's Patches in Ligated Ileal Loops: An Ultrastructural Study

Cloning and characterization of the gene encoding the z66 antigen of Salmonella enterica serovar Typhi

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