Invasive species management on military lands : clustered regularly interspaced short palindromic repeat/CRISPR-associated protein 9 (CRISPR/Cas9)-based gene drives

Gong, Ping

doi:10.21079/11681/22721

Cited by 2 publications

(2 citation statements)

References 102 publications

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“…Molecular tools have the potential to address climate mismatches by using them to identify cold tolerance mechanisms and then manipulate them to overcome thermal limitations of agents. In fact, the use of advanced biotechnology has received recent attention for its potentially significant contribution to invasive species management [133][134][135][136]. Modern molecular approaches such as synthetic and systems biology, in combination with advanced genomics, gene silencing, and genetic engineering, have been employed to identify, characterize, activate and engineer/transform specific cold resistance genes in plants and animals [137][138][139].…”

Section: Non-classical Biological Control: Molecular Approaches To Addressing Climate Mismatchmentioning

confidence: 99%

“…There are numerous reports on breeding cold tolerance in crop and horticultural plants for temperate locations [138,152]. The recent emergence and advancement of clustered regularly interspaced short palindromic repeat (CRISPR) technology [153] has revolutionized genome editing and genetic engineering [133][134][135]. The CRISPR system is based on an RNA-guided endonuclease discovered in bacteria [154], repurposed by researchers for high-fidelity genetic engineering [155].…”

Section: Enhancing Cold Hardinessmentioning

confidence: 99%

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Climate Mismatch between Introduced Biological Control Agents and Their Invasive Host Plants: Improving Biological Control of Tropical Weeds in Temperate Regions

Harms

Knight

Pratt

et al. 2021

Insects

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Many weed biological control programs suffer from large-scale spatial variation in success due to restricted distributions or abundances of agents in temperate climates. For some of the world’s worst aquatic weeds, agents are established but overwintering conditions limit their survival in higher latitudes or elevations. The resulting need is for new or improved site- or region-specific biological control tools. Here, we review this challenge with a focus on low-temperature limitations of agents and propose a roadmap for improving success. Investigations across spatial scales, from global (e.g., foreign exploration), to local (selective breeding), to individual organisms (molecular modification), are discussed. A combination of traditional (foreign) and non-traditional (introduced range) exploration may lead to the discovery and development of better-adapted agent genotypes. A multivariate approach using ecologically relevant metrics to quantify and compare cold tolerance among agent populations is likely required. These data can be used to inform environmental niche modeling combined with mechanistic modeling of species’ fundamental climate niches and life histories to predict where, when, and at what abundance agents will occur. Finally, synthetic and systems biology approaches in conjunction with advanced modern genomics, gene silencing and gene editing technologies may be used to identify and alter the expression of genes enhancing cold tolerance, but this technology in the context of weed biological control has not been fully explored.

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Section: Non-classical Biological Control: Molecular Approaches To Addressing Climate Mismatchmentioning

confidence: 99%

Section: Enhancing Cold Hardinessmentioning

confidence: 99%

Climate Mismatch between Introduced Biological Control Agents and Their Invasive Host Plants: Improving Biological Control of Tropical Weeds in Temperate Regions

Harms

Knight

Pratt

et al. 2021

Insects

Self Cite

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Fixing Pitx3 gene Mutation with CRISPR-Cas9 Intervention in Zygotic Aphakia-Prone Mice – A Research Protocol

2021

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Introduction: Despite the drawbacks of gene-editing, CRISPR-Cas9 remains a versatile tool for editing mutated genes. The mutated Pitx3 gene may cause aphakia in mice and Parkinsonism in humans. Pitx3 gene codes for the Pitx3 transcription factor involved in many tasks including the differentiation of dopaminergic neurons in the substantia nigra, tyrosine hydroxylase expression, dopamine transporter and lens development. We aim to edit mutated Pitx3 genes using CRISPR-Cas9 in embryonic-dopaminergic progenitor-neurons of mice and compare to wildtypes (WT) that do not undergo CRISPR-Cas9 intervention. Methods: We will use 5 female mice, 2 homozygous for mice aphakia/Pitx3 mutation (Ak) and 3 standard pseudo-pregnant females. 2-male homozygous (+/+ Ak) mice with Ak will mate with the aphakic females (+/+ Ak). Dopamine levels in both (aphakic/non-aphakic) mice will be determined. We will extract embryonic stem cells (ESC) from aphakic female's uterus and treat with CRISPR-Cas9 and homology-directed repair (HDR). Both treated and untreated ESC will be divided between 3 pseudo-pregnant females. Post-parturition, intervention success will be assessed using presence of optical lens, dopamine levels and restriction sequence expression in treated/untreated progeny. Results: We expect to observe that the untreated ESC zygotes (no CRISPR-Cas9 and HDR treatment) will not express normal Pitx3. This will result in low dopamine levels (inappropriate dopamine levels for their age group) and an aphakic mice progeny. Likewise, treated ESC zygotes will express normal Pitx3, hence have normal dopamine levels and nonaphakic phenotype. Discussion: Progeny of both groups (treated/non-treated) will be examined for aphakia, dopamine levels and restriction sequence. Dopamine levels of treated mice will be compared to dopamine levels of WT mice. Should none of the treated progeny develop lenses or have normal dopamine levels, these mice will be sacrificed to target the restriction sequence in the inserted genehelping to evaluate why the inserted gene is unexpressed. Conclusion:This research examines CRISPR-Cas9 and HDR use on embryos with mutated genes that impair lens development and dopaminergic-neuron differentiation in humans and mice. It has potential of qualifying for clinical trials as the technique may be used to fix heritable disease-inducing genes that decrease longevity and quality of life in humans.

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Invasive species management on military lands : clustered regularly interspaced short palindromic repeat/CRISPR-associated protein 9 (CRISPR/Cas9)-based gene drives

Cited by 2 publications

References 102 publications

Climate Mismatch between Introduced Biological Control Agents and Their Invasive Host Plants: Improving Biological Control of Tropical Weeds in Temperate Regions

Climate Mismatch between Introduced Biological Control Agents and Their Invasive Host Plants: Improving Biological Control of Tropical Weeds in Temperate Regions

Fixing Pitx3 gene Mutation with CRISPR-Cas9 Intervention in Zygotic Aphakia-Prone Mice – A Research Protocol

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