Invasive phenotype observed in 1,3-bis(2-chloroethyl)-1-nitrosourea—resistant sublines of 9L rat glioma cells: a tumor model mimicking a recurrent malignant glioma

Saito, Ryuta; Bringas, John; Mirek, Hanna; Berger, Mitchel S.; Bankiewicz, Krzysztof S.

doi:10.3171/jns.2004.101.5.0826

Cited by 17 publications

(15 citation statements)

References 22 publications

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“…4C, is very clear and was used for delineation. Previous experiments comparing distribution of co-infused fluorescent liposomes on histology with distribution of gadolinium-loaded liposomes on MRI validated this method (Saito et al, 2004a, submitted for publication). Sections from brains injected with coomassie blue or trypan blue were scanned using a scanner (Perfection 1660 photo; Epson, Long Beach, CA).…”

Section: Evaluation Of Volume Of Distribution After Convection-enhancmentioning

confidence: 76%

“…Moreover, distribution was identical regardless of the agent encapsulated within the liposomes, supporting the use of neutral liposomes as a drug carrier for predictable and highly efficient local delivery. In a previous report, we demonstrated a strategy for monitoring liposomal distribution using MRI (Saito et al, 2004a). Considering the identical distribution of neutral liposomes, this monitoring system can be used for rapid screening of tissue distribution by co-infusing a liposome-encapsulated compound of interest and liposomes containing gadolinium.…”

Section: Discussionmentioning

confidence: 99%

“…A sagittal incision was made through the skin to expose the cranium, and a burr hole was made in the skull 0.5 mm anterior and 3 mm lateral to the bregma with a small dental drill. Infusions were performed at a depth of 4.5 mm from the brain surface, using the CED method described in our previous studies (Saito et al, 2004a;Lieberman et al, 1995). Briefly, an infusion cannula connected to a Hamilton syringe (Hamilton, Reno, NV) was attached to a rate-controllable microinfusion pump (Bioanalytical Systems, Lafayette, IN).…”

Section: Surgery and The Convection-enhanced Delivery Proceduresmentioning

confidence: 99%

“…In this study, we demonstrate differences in distributions for molecules displaying varying physical and chemical properties. In previous reports (Mamot et al, 2004;Saito et al, 2004a), we demonstrated the detection of gadolinium-loaded liposomes in brain tumors after CED, using magnetic resonance imaging (MRI). Because our present focus is to develop a CED strategy for the delivery of liposomes encapsulating various agents, including chemotherapeutic drugs or therapeutic genes, we evaluated the effect of size, surface charge and steric stabilization of liposomes on delivery by using CED.…”

Section: Introductionmentioning

confidence: 97%

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Tissue affinity of the infusate affects the distribution volume during convection-enhanced delivery into rodent brains: Implications for local drug delivery

Saito

Krauze

Noble

et al. 2006

Journal of Neuroscience Methods

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Section: Evaluation Of Volume Of Distribution After Convection-enhancmentioning

confidence: 76%

Section: Discussionmentioning

confidence: 99%

Section: Surgery and The Convection-enhanced Delivery Proceduresmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

See 2 more Smart Citations

Tissue affinity of the infusate affects the distribution volume during convection-enhanced delivery into rodent brains: Implications for local drug delivery

Saito

Krauze

Noble

et al. 2006

Journal of Neuroscience Methods

Self Cite

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“…Previous studies have described experimental gliomas with invasive and aggressive growth patterns but no comparison between treatments in models with disparate invasiveness have been presented. 3,4 Our laboratory has published several studies investigating therapeutic effects of interferon (IFN)g transformed tumor cell vaccines using the N32 rat brain tumor model. 5,6 In this study, we investigated whether IFNg based immunotherapy would also be able to eliminate the more invasive N29 tumor.…”

mentioning

confidence: 99%

Immunizations With IFNγ Secreting Tumor Cells can Eliminate Fully Established and Invasive Rat Gliomas

Janelidze¹,

Bexell²,

Badn³

et al. 2009

Journal of Immunotherapy

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Immunotherapy of malignant primary brain tumors holds the potential to improve the dismal prognosis after current clinical therapy. Although immunotherapy of experimental gliomas has been demonstrated to have the capacity to cure intracerebral tumors no convincing effects of immunotherapy have been shown in clinical trials. One reason for this could be that some of the models used do not display full features of human glioblastomas. The N29 rat gliomas exhibited all the histologic features of human glioblastoma multiforme including nuclear atypia, mitotic figures, necrosis, and diffuse infiltration into the normal brain tissue. Surprisingly, immunotherapy with autologous interferon gamma producing tumor cells against preestablished intracerebral N29 tumors yielded a higher cure rate than immunotherapy against less invasive tumors. Furthermore, when immunizations were postponed until day 5 after tumor establishment 50% of the animals survived. When immunizations were postponed until day 11 after tumor establishment no glioma-bearing animals were cured but survival was significantly prolonged. The superior effect of immunotherapy in the invasive N29 model compared with the less invasive tumors could depend on combined effects of up-regulation of major histocompatibility complex I and induction of major histocompatibility complex II plus CD80 after transfection and irradiation of the tumor cells used for immunizations. This study demonstrates that immunotherapy against experimental brain tumors indeed is feasible even against highly invasive and established tumors. These results strengthen the translational potential of immunotherapy against malignant brain tumors.

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Proton and sodium MRI assessment of emerging tumor chemotherapeutic resistance

et al. 2006

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The ultimate goal of any cancer therapy is to target the elimination of neoplastic cells. Although newer therapeutic strategies are in constant development, therapeutic assessment has been hampered by the inability to assess, rapidly and quantitatively, efficacy in vivo. Diffusion imaging and, more recently, sodium MRI have demonstrated their distinct abilities to detect therapyinduced alterations in tumor cellularity, which has been demonstrated to be indicative of therapeutic efficacy. More importantly, both imaging modalities detect tumor response much earlier than traditional methodologies that rely on macroscopic volumetric changes. In this study, the correlation between tumor sodium and diffusion was further tested to demonstrate the sensitivity of sodium imaging to gauge tumor response to therapy by using a 9L rat gliosarcoma treated with varying doses of BCNU [1,3-bis(2-chloroethyl)-1-nitrosourea]. This orthotopic model has been demonstrated to display variability in response to BCNU therapy where initial insult has been shown to lead to drug-resistance. In brief, a single 26.6 mg/kg BCNU dose yielded dramatic responses in both diffusion and sodium MRI. However, a second equivalent BCNU dose yielded a much smaller change in diffusion and sodium, suggesting a drop in tumor sensitivity to BCNU. The MRI responses of animals treated with 13.3 mg/kg BCNU were much lower and similar responses were observed after the initial and secondary applications of BCNU. Furthermore, these results were further validated using volumetric measurements of the tumor and also ex vivo determination of tumor sensitivity to BCNU. Overall, these experiments demonstrate the sensitivity and applicability of sodium and diffusion MRI as tools for dynamic assessment of tumor response to therapy.

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Invasive phenotype observed in 1,3-bis(2-chloroethyl)-1-nitrosourea—resistant sublines of 9L rat glioma cells: a tumor model mimicking a recurrent malignant glioma

Cited by 17 publications

References 22 publications

Tissue affinity of the infusate affects the distribution volume during convection-enhanced delivery into rodent brains: Implications for local drug delivery

Tissue affinity of the infusate affects the distribution volume during convection-enhanced delivery into rodent brains: Implications for local drug delivery

Immunizations With IFNγ Secreting Tumor Cells can Eliminate Fully Established and Invasive Rat Gliomas

Proton and sodium MRI assessment of emerging tumor chemotherapeutic resistance

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