Invasive Disease Due to Group B Streptococcal Infection in Adults: Results From a Canadian, Population‐Based, Active Laboratory Surveillance Study—1996

Tyrrell, Gregory J.; Senzilet, Linda D.; Spika, John S.; Kertesz, Daniel A.; Alagaratnam, Merrilyn; Lovgren, Marguerite; Talbot, James

doi:10.1086/315699

Cited by 132 publications

(136 citation statements)

References 23 publications

Supporting

Mentioning

108

Contrasting

Unclassified

Order By: Relevance

“…Streptococcus agalactiae (group B streptococcus, GBS) is the most common cause of neonatal (Ekelund & Konradsen, 2004) and obstetric sepsis, and is an increasingly important cause of septicaemia in the elderly and in immunocompromised patients (Schuchat et al, 2002;Tyrrell et al, 2000). There has been considerable progress in the development of conjugate polysaccharide GBS vaccines (Paoletti & Madoff, 2002).…”

Section: Introductionmentioning

confidence: 99%

Simultaneous detection and serotype identification of Streptococcus agalactiae using multiplex PCR and reverse line blot hybridization

Kong

Gilbert

2005

Journal of Medical Microbiology

View full text Add to dashboard Cite

Streptococcus agalactiae (group B streptococcus, GBS) is an important cause of sepsis in neonates and their mothers, and the elderly and immunocompromised patients. Ongoing surveillance to monitor GBS serotype distribution is needed to guide the development and assess the feasibility of GBS conjugate vaccines. The authors previously developed a molecular serotype identification method based on serotype-specific PCR and partial sequencing of cps genes. In this study, a novel 10-primer pair multiplex PCR and reverse line blot (mPCR/RLB) hybridization assay was developed for simultaneous detection and serotype identification of all nine GBS serotypes. For all 316 GBS isolates tested the mPCR/RLB results corresponded with those of conventional serotyping and individual serotype-specific PCR, and the method was more convenient and practical than either alternative.

show abstract

Section: Introductionmentioning

confidence: 99%

Simultaneous detection and serotype identification of Streptococcus agalactiae using multiplex PCR and reverse line blot hybridization

Kong

Gilbert

2005

Journal of Medical Microbiology

View full text Add to dashboard Cite

show abstract

“…It is also an important cause of morbidity in pregnant women and non-pregnant adults with underlying medical conditions (3)(4)(5). GBS colonizes the human gastrointestinal and genitourinary tracts and may cause chorioamnionitis and urinary tract infection in pregnant women and a range of invasive infections in elderly and immunocompromised adults (1, 6 -8).…”

mentioning

confidence: 99%

Crystal Structure of the N-terminal Domain of the Group B Streptococcus Alpha C Protein

Auperin

Bolduc

Baron

et al. 2005

Journal of Biological Chemistry

View full text Add to dashboard Cite

Group B Streptococcus (GBS) is the leading cause of bacterial pneumonia, sepsis, and meningitis among neonates and an important cause of morbidity among pregnant women and immunocompromised adults. Invasive diseases due to GBS are attributed to the ability of the pathogen to translocate across human epithelial surfaces. The alpha C protein (ACP) has been identified as an invasin that plays a role in internalization and translocation of GBS across epithelial cells. The soluble N-terminal domain of ACP (NtACP) blocks the internalization of GBS. We determined the 1.86-Å resolution crystal structure of NtACP comprising residues Ser 52 through Leu 225 of the full-length ACP. NtACP has two domains, an N-terminal ␤-sandwich and a C-terminal three-helix bundle. Structural and topological alignments reveal that the ␤-sandwich shares structural elements with the type III fibronectin fold (FnIII), but includes structural elaborations that make it unique. We have identified a potential integrin-binding motif consisting of Lys-Thr-Asp 146 , Arg 110 , and Asp 118 . A similar arrangement of charged residues has been described in other invasins. ACP shows a heparin binding activity that requires NtACP. We propose a possible heparin-binding site, including one surface of the three-helix bundle, and nearby portions of the sandwich and repeat domains. We have validated this prediction using assays of the heparin binding and cell-adhesion properties of engineered fragments of ACP. This is the first crystal structure of a member of the highly conserved Gram-positive surface alpha-like protein family, and it will enable the internalization mechanism of GBS to be dissected at the atomic level.Group B Streptococcus (GBS) 1 (Streptococcus agalactiae) remains the leading cause of invasive bacterial diseases in neonates, despite its decline in prevalence during the last decade because of intrapartum chemoprophylatic therapy (1, 2). It is also an important cause of morbidity in pregnant women and non-pregnant adults with underlying medical conditions (3-5). GBS colonizes the human gastrointestinal and genitourinary tracts and may cause chorioamnionitis and urinary tract infection in pregnant women and a range of invasive infections in elderly and immunocompromised adults (1, 6 -8). During labor and delivery, GBS may be transmitted to neonates, causing pneumonia, sepsis, or meningitis. Four to six percent of all neonatal GBS infections result in death (6, 9). In vitro, GBS adheres to (10, 11), internalizes within (12-14), and translocates across (15) intact human epithelial and endothelial cells. Little is known about the bacterial components that allow this pathogen to adhere to and penetrate cellular membranes. Previous studies suggest that surface proteins are significantly involved in the process (11, 16). The surface-expressed GBS alpha C protein (ACP) has been shown to act as an invasin (15). ACP is the prototype of a family of surface-expressed proteins containing long tandem repeats (alpha-like proteins (Alp)). Members of this famil...

show abstract

“…Risk factors also seem to vary between younger and older adults (≥65 years). DM, malignancy, and skin disease were commonly encountered in younger adults, whereas cardiac disease, bedridden state, and nursing home residence were more frequently seen in older patients [20]. In contrast, nephrolithiasis and prior urologic surgery were strong independent risk factors for retroperitoneal abscesses (RPAs), according to one 26-year retrospective study [21].…”

Section: Discussionmentioning

confidence: 99%

Invasive Group B streptococcus: multiloculated perinephric abscesses treated with percutaneous drainage

Peña-Garcia

Shaikh

Lacasse

2018

Journal of Community Hospital Internal Medicine Perspectives

View full text Add to dashboard Cite

Group B streptococcus infections (GBSI) are commonly associated with neonates and pregnant women, but may also affect nonpregnant adults. Among its spectrum of manifestations, perinephric abscess (PA) is exceedingly rare. Comorbid conditions such as diabetes mellitus (DM) and immunosuppression increase the risk of GBSI. We describe a 61-year-old Vietnamese man with compensated alcoholic cirrhosis, who presented with acute encephalopathy following subacute, progressive abdominal pain. He was afebrile and hemodynamically stable. Laboratory data were remarkable for leukocytosis, thrombocytopenia, azotemia, and pyuria. He was found to have two right-sided PA measuring 15 × 10 × 11 cm and 4.6 × 2.7 × 7.8 cm, requiring interval placement of multiple percutaneous drains. Culture from abscesses revealed beta-hemolytic Group B streptococcus (GBS). His course was complicated by contiguous spread to abdominal wall and paraspinal musculature, as well as a new diagnosis of type 2 DM. Along with drainage, a prolonged course of intravenous antimicrobial treatment led to abscess resolution. Given the rising number of unusual clinical presentations of GBSI, this bacteria should be considered as a part of the microbiological differential diagnosis of PA, especially in conditions leading to immunosuppression.

show abstract

Invasive Disease Due to Group B Streptococcal Infection in Adults: Results From a Canadian, Population‐Based, Active Laboratory Surveillance Study—1996

Cited by 132 publications

References 23 publications

Simultaneous detection and serotype identification of Streptococcus agalactiae using multiplex PCR and reverse line blot hybridization

Simultaneous detection and serotype identification of Streptococcus agalactiae using multiplex PCR and reverse line blot hybridization

Crystal Structure of the N-terminal Domain of the Group B Streptococcus Alpha C Protein

Invasive Group B streptococcus: multiloculated perinephric abscesses treated with percutaneous drainage

Contact Info

Product

Resources

About