Invasive cervical cancers from women living in the United States or Botswana: differences in human papillomavirus type distribution

Ermel, Aaron; Ramogola‐Masire, Doreen; Zetola, Nicola M.; Tong, Yan; Qadadri, Brahim; Azar, Marwan M.; Brown, Darron R.

doi:10.1186/1750-9378-9-22

Cited by 12 publications

(26 citation statements)

References 21 publications

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“…This finding, however, is higher than the report from a comparative study of HIV DNA rate in cervical tumor specimens of 31.5% in Botswana versus 3.9% in the United States. 15 The finding of high level of HIV coinfection correlates with the higher seroprevalence rate of 40% to 50% among females aged 30 to 50 years, 16 who comprised 52% of the study population. Several studies report variable HIVpositive seroprevalence among cervical cancer patients, varying from 1.6% in Hong Kong, 15% in Kenyas, and 21% in Durban, South Africa.…”

Section: Discussionmentioning

confidence: 79%

Predictors of Locally Advanced Disease at Presentation and Clinical Outcomes Among Cervical Cancer Patients Admitted at a Tertiary Hospital in Botswana

Nassali¹,

Melese²,

Nkuba

et al. 2018

International Journal of Gynecological Cancer

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Section: Discussionmentioning

confidence: 79%

Predictors of Locally Advanced Disease at Presentation and Clinical Outcomes Among Cervical Cancer Patients Admitted at a Tertiary Hospital in Botswana

Nassali¹,

Melese²,

Nkuba

et al. 2018

International Journal of Gynecological Cancer

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“…Acquisition of archived, paraffin-embedded blocks of formalin-fixed cervical cancer specimens from women residing in Indianapolis, Indiana (U.S.) and from Botswana was previously described [7]. Case selection was done by working backwards in time among archived paraffin blocks.…”

Section: Methodsmentioning

confidence: 99%

“…Thin-section polymerase chain reaction (PCR) of specimens from Kenyan women was performed exactly as described in a prior report of ICC cases from women living in Botswana and the U.S. [7]. Briefly, four to six paraffin-embedded tissue sections were scraped from slides prepared from each block using a sterile blade.…”

Section: Methodsmentioning

confidence: 99%

“…Briefly, four to six paraffin-embedded tissue sections were scraped from slides prepared from each block using a sterile blade. DNA extraction was then performed as previously described [7]. …”

Section: Methodsmentioning

confidence: 99%

“…Surveillance and better understanding of local epidemiology must be encouraged before selecting the HPV vaccine of choice for a specific country. The object of the study was therefore to compare HPV typing data from a previously published study of ICC cases in the United States and Botswana [7] to additional cases identified in a second sub-Saharan country, Kenya, and to determine the HPV type distribution in invasive cervical cancers from HIV-infected women living in these countries.…”

Section: Introductionmentioning

confidence: 99%

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Invasive cervical cancers in the United States, Botswana and Kenya: HPV type distribution and health policy implications

Ermel

Qadadri

Tong

et al. 2016

Infect Agents Cancer

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BackgroundMore deaths occur in African women from invasive cervical cancer (ICC) than from any other malignancy. ICC is caused by infection with oncogenic types of human papillomavirus (HPV). Co-infection with the human immunodeficiency virus (HIV) accelerates the natural history of ICC, and may influence the HPV type distribution. Because HPV vaccines are available, this malignancy is theoretically preventable, but the vaccines are largely type-specific in protection against infection. Data on specific HPV types causing ICC in African women is limited, and many studies utilized swab samples rather than actual cancer tissue. A previous study using archived, ICC tissue from women in Botswana identified an unusual HPV type distribution. A similar study was therefore performed in a second sub-Saharan country to provide additional information on the HPV type distribution in ICC.MethodsArchived, formalin-fixed, paraffin-embedded ICCs were acquired from women in the United States, Kenya, or Botswana. DNA was extracted and HPV genotyping performed by Roche Linear Array. HIV sequences were identified in ICCs by PCR.ResultsHPV types 16 or 18 (HPV 16/18) were identified in 93.5 % of HPV-positive ICCs from the U.S., 93.8 % from Kenya, and 61.8 % from Botswana (p < 0.0001). Non-HPV 16/18 types were detected in 10.9 % of HPV-positive cancers from the U.S., 17.2 % from Kenya, and 47.8 % from Botswana (p < 0.0001). HIV was detected in 2.2, 31.5, and 32.4 % from ICCs from the U.S., Kenya, or Botswana, respectively (p = 0.0002). The distribution of HPV types was not significantly different between HIVinfected or HIV-uninfected women. The percentages of ICCs theoretically covered by the bivalent/quadrivalent HPV vaccines were 93.5, 93.9, and 61.8 % from the U.S., Kenya and Botswana, respectively, and increased to 100, 98, and 77.8 % for the nanovalent vaccine.ConclusionsHPV 16/18 caused most ICCs from the U.S. and western Kenya. Fewer ICCs contained HPV 16/18 in Botswana. HIV co-infection did not influence the HPV type distribution in ICCs from African women from the two countries. Available HPV vaccines should provide protection against most ICCs in the U.S. and Kenya. The recently developed nanovalent vaccine may be more suitable for countries where non-HPV 16/18 types are frequently detected in ICC.Electronic supplementary materialThe online version of this article (doi:10.1186/s13027-016-0102-9) contains supplementary material, which is available to authorized users.

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Human papillomavirus genotypes in women with invasive cervical cancer with and without human immunodeficiency virus infection in Botswana

Tawe

MacDuffie

Narasimhamurthy

et al. 2019

Intl Journal of Cancer

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Cervical cancer remains a significant cause of morbidity and mortality in women worldwide and is the leading cause of cancer-related death in Botswana. It is well established that women with HIV have a higher risk of persistent HPV infection leading to cervical cancer. We assessed HPV prevalence and genotype distribution in 126 tissue specimens from confirmed invasive cervical cancer cases using Abbott real-time PCR assay. Overall, 88(69.8%) women were HIV-infected. Fifty-seven (64.8%) of the HIV-infected women had a baseline CD4 + count ≥ 350 cells/μl, and 82(93.2%) were on antiretroviral therapy at the time of cervical cancer diagnosis. The median age of HIV-infected patients was signficantly younger than that of HIV-uninfected patients (p < 0.001). HPV DNA was detected in all of 126(100%) of tissues analysed in our study. The HPV genotypes identfied included the HPV-16 (75.4%), HPV-18 (28.6%) and other high-risk (hr) HPV genotypes

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Invasive cervical cancers from women living in the United States or Botswana: differences in human papillomavirus type distribution

Cited by 12 publications

References 21 publications

Predictors of Locally Advanced Disease at Presentation and Clinical Outcomes Among Cervical Cancer Patients Admitted at a Tertiary Hospital in Botswana

Predictors of Locally Advanced Disease at Presentation and Clinical Outcomes Among Cervical Cancer Patients Admitted at a Tertiary Hospital in Botswana

Invasive cervical cancers in the United States, Botswana and Kenya: HPV type distribution and health policy implications

Human papillomavirus genotypes in women with invasive cervical cancer with and without human immunodeficiency virus infection in Botswana

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