Invasive Aspergillosis in Intensive Care Unit Patients in Iran

Hedayati, Mohammad Taghi; Khodavaisy, Sadegh; Amini, Masoud; Omran, Saeid Mahdavi; Habibi, Mohammad Reza

doi:10.14712/18059694.2014.24

Cited by 13 publications

(7 citation statements)

References 34 publications

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“…In previous studies (7,(12)(13)(14), using a cut-off index of ≥ 1, the sensitivity and specificity values of GM detection in BAL fluid were 85% to 100% (14,15). Therefore, GMI 1.0 or greater in 2 consecutive samples was considered as positive in this study.…”

Section: Resultsmentioning

confidence: 91%

Investigating the Presence of Aspergillus fumigatus and A. flavus Using Galactomannan Enzyme Assay and TaqMan Real-Time PCR Technique

Roudbarmohammadi

Khorashad

Forouzandeh-Moghadam

et al. 2018

Jundishapur J Microbiol

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Background: Invasive aspergillosis (IA), a serious and fatal disease, is caused by numerous opportunistic fungi including Aspergillus species. Lack of early diagnosis and delay in treatment lead to the rapid spread of the infection and relapse, increase treatment costs, and ultimately cause death. Objectives: This study aimed at investigating the presence of Aspergillus species by Galactomannan EIA (GM) and TaqMan q PCR methods. Methods: Fluid samples of bronchoalveolar lavage (BAL) were collected from 89 patients, who were at risk for IA and underwent a bronchoscopy at Shariati hospital in Tehran, Iran. The specimens were examined using direct examination, culture methods, and GM and TaqMan real-time PCR. Results: A total of 23 samples were found to be positive in direct examination, 7 were identified as A. fumigatus, and 11 were positive as A.flavus in culture assays, 27 were GM positive, and 29 were positive with PAN Aspergillus probe. Moreover, 7 samples were positive with A. fumigatus probe and 11 were positive with A. flavus probe. Negative predictive value (NPV), sensitivity, positive predictive value

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Section: Resultsmentioning

confidence: 91%

Investigating the Presence of Aspergillus fumigatus and A. flavus Using Galactomannan Enzyme Assay and TaqMan Real-Time PCR Technique

Roudbarmohammadi

Khorashad

Forouzandeh-Moghadam

et al. 2018

Jundishapur J Microbiol

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“…A. fumigatus is globally found in the hospital environments and most frequently isolated from patients with IA or aspergilloma [ 28 - 34 ]. the highest airborne Aspergillus conidia concentrations due to the construction inside or surroundings area of the hospitals as well as problems in the ventilation or conditioner systems have proposed the environment to be the potential source of infection [ 35 , 36 ].…”

Section: Discussionmentioning

confidence: 99%

Molecular typing of clinical and environmental Aspergillus fumigatus isolates from Iran using microsatellites

Badali

Shokohi

Khodavaisy

et al. 2021

CMM

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Background and Purpose: Because of the growing incidence of Aspergillus infection,typing methods of Aspergillus species are increasingly being used. Accordingly,studying the spread and population dynamics of strains isolating from clinical and environment, from a single host to large scale ecosystems is definitely needed. In the current study, we carried out a genetic analysis of nine microsatellite loci in isolates from different regions of Iran to compare and explore the genetic diversity between environmental and clinical A. fumigatus strains. Materials and Methods: Sixty-six clinical (n=43) and environmental (n= 23) isolates of A. fumigatus, have collected from six cities of Iran. All A. fumigatus isolates identified based on macroscopic and microscopic characters, the ability to grow at above 45°C, and confirmed using DNA sequencing of the partial b-tubulin gene.Sixty-six A. fumigatus isolates were subjected by microsatellite typing using three separate multiplex PCRs with a panel of nine short tandem repeats (STR) to evaluate the genetic relatedness. Results: The STR typing of 66 A. fumigatus isolates revealed 38 distinct genotypes distributed among environmental and clinical isolates. We identified 12 clones including 40 different isolates representing 60% of all isolates tested, which each clone included 2–7 isolates. Conclusion: The STR typing is considered as a valuable tool with excellent discriminatory power to study the molecular epidemiology and genotypic diversity of A.fumigatus isolates. These findings show that the high genetic diversity observed of Iranian A. fumigatus isolates with those outside Iran and formed a separate cluster.

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“…In a study conducted by Hedayati et al in Iran, out of 36 suspected patients to IA, 36.1% of cases showed IA which were categorized as 4 cases of possible IA and 9 of probable IA. 35 In the present study we applied GM detection in BAL. Some authors suggested that it can be beneficial to detect GM in BAL fluid for IA diagnosis.…”

Section: Discussionmentioning

confidence: 99%

<p>Fungal Isolates of the Respiratory Tract in Symptomatic Patients Hospitalized in Pulmonary Units: A Mycological and Molecular Epidemiologic Study</p>

Rafat¹,

Hashemi²,

Ashrafi³

et al. 2020

JMDH

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Introduction: Fungal respiratory infections are being recognized with increasing frequency in parallel with an expanding population of immunocompromised patients. In most cases, colonization is the first step in the progression to pulmonary fungal infection. This study was designed to evaluate the distribution of fungal elements in the respiratory tract of symptomatic patients hospitalized in pulmonary units. Methods: This descriptive cross-sectional study was carried out over a period of two years, from October 2017 to October 2019 in Guilan province, located in Iran's northern region. In the current study, bronchoalveolar lavage or sputum specimens were collected. All samples were analyzed by direct microscopy using KOH 10% and culture. Fungal identification was accomplished by internal transcribed spacer (ITS) and beta-tubulin sequencing. Also, in patients suspected of invasive pulmonary aspergillosis, BAL specimens were tested for galactomannan (GM) antigen. Results: A total of 384 lung specimens (192 bronchoalveolar lavage (BAL) and 192 sputum samples) were obtained from symptomatic patients hospitalized in pulmonary units. Of these, 137 (35.67%) were positive in direct examination and culture. Among the 137 positive cases, most isolates were from male patients 86 (62.77%) and most of them were between 46 and 72 years. Candida albicans (37.22%) and Candida tropicalis (21.89%) represent the two most commonly isolated species in the current study. Cough (94.16%), dyspnea (81.02%), purulent sputum (62.04%) and weight loss (56.2%) were the predominant symptoms and tuberculosis (24.81%), chemotherapy (21.89%) and diabetes mellitus (19.70%) were the predominant underlying conditions. Also, 5 cases of invasive pulmonary aspergillosis and 1 case of mucormycosis were diagnosed. Conclusion: Candida albicans was the most common fungal species isolated from symptomatic patients hospitalized in pulmonary units. Tuberculosis, chemotherapy and diabetes mellitus were important underlying conditions for pulmonary fungal colonization and/or infection.

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Invasive Aspergillosis in Intensive Care Unit Patients in Iran

Cited by 13 publications

References 34 publications

Investigating the Presence of Aspergillus fumigatus and A. flavus Using Galactomannan Enzyme Assay and TaqMan Real-Time PCR Technique

Investigating the Presence of Aspergillus fumigatus and A. flavus Using Galactomannan Enzyme Assay and TaqMan Real-Time PCR Technique

Molecular typing of clinical and environmental Aspergillus fumigatus isolates from Iran using microsatellites

<p>Fungal Isolates of the Respiratory Tract in Symptomatic Patients Hospitalized in Pulmonary Units: A Mycological and Molecular Epidemiologic Study</p>

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