Invasive amebiasis: challenges in diagnosis in a non-endemic country (Kuwait).

Hira, Persotum Ravjee; Iqbal, Jamshaid; Al-Ali, Faiza Mohamed; Philip, Reeni; Grover, Saroj Bala; D'almeida, Emmanuel; Al-Eneizi, Abdulaziz A.

doi:10.4269/ajtmh.2001.65.341

Cited by 30 publications

(15 citation statements)

References 11 publications

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“…It may also be useful in the evaluation of intestinal and extraintestinal infections where amebiasis is suspected but organisms cannot be detected in feces (152). A microtiter ELISA to detect antibodies to E. histolytica (LMD Laboratories, Inc., Carlsbad, CA) has been shown to be 97.9% sensitive and 94.8% specific for detection of E. histolytica antibodies in ALA patients (84). Serum IgG antibodies persist for years after E. histolytica infection, whereas the presence of IgM antibodies is shortlived and can be detected during the present or current infection.…”

Section: Antibody Detection Testsmentioning

confidence: 99%

Laboratory Diagnostic Techniques for Entamoeba Species

et al. 2007

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SUMMARY The genus Entamoeba contains many species, six of which (Entamoeba histolytica, Entamoeba dispar, Entamoeba moshkovskii, Entamoeba polecki, Entamoeba coli, and Entamoeba hartmanni) reside in the human intestinal lumen. Entamoeba histolytica is the causative agent of amebiasis and is considered a leading parasitic cause of death worldwide in humans. Although recent studies highlight the recovery of E. dispar and E. moshkovskii from patients with gastrointestinal symptoms, there is still no convincing evidence of a causal link between the presence of these two species and the symptoms of the host. New approaches to the identification of E. histolytica are based on detection of E. histolytica-specific antigen and DNA in stool and other clinical samples. Several molecular diagnostic tests, including conventional and real-time PCR, have been developed for the detection and differentiation of E. histolytica, E. dispar, and E. moshkovskii in clinical samples. The purpose of this review is to discuss different methods that exist for the identification of E. histolytica, E. dispar, and E. moshkovskii which are available to the clinical diagnostic laboratory. To address the need for a specific diagnostic test for amebiasis, a substantial amount of work has been carried out over the last decade in different parts of the world. The molecular diagnostic tests are increasingly being used for both clinical and research purposes. In order to minimize undue treatment of individuals infected with other species of Entamoeba such as E. dispar and E. moshkovskii, efforts have been made for specific diagnosis of E. histolytica infection and not to treat based simply on the microscopic examination of Entamoeba species in the stool. The incorporation of many new technologies into the diagnostic laboratory will lead to a better understanding of the public health problem and measures to control the disease.

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Section: Antibody Detection Testsmentioning

confidence: 99%

Laboratory Diagnostic Techniques for Entamoeba Species

et al. 2007

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“…These nine patients may be at an early stage of the infection and therefore had undetectable levels of anti-E. histolytica antibodies. According to previous reports, serology may be negative in patients in the acute phase within 7-10 days postinfection (Hira et al, 2001;Fotedar et al, 2007). At this stage, liver abscess may have developed and thus early detection is crucial to avoid complications.…”

Section: Discussionmentioning

confidence: 92%

Application of Real-Time Polymerase Chain Reaction in Detection ofEntamoeba histolyticain Pus Aspirates of Liver Abscess Patients

Othman

Mohamed

Verweij

et al. 2010

Foodborne Pathogens and Disease

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Entamoeba histolytica is the second major cause of liver abscess disease in humans, particularly in developing countries. Recently, DNA molecular-based methods have been employed to enhance the detection of E. histolytica in either pus or stool specimens. In this study, the results of real-time polymerase chain reaction (PCR) to detect E. histolytica DNA in pus from liver abscess cases were compared with those of indirect hemagglutination assay on the corresponding serum samples. Bacterial cultures were also performed on the pus samples for the diagnosis of pyogenic liver abscess. The real-time PCR detected E. histolytica DNA in 23 of 30 (76.7%) pus samples, when compared with 14 of 30 (46.7%) serum samples in which anti-Entamoeba antibodies were detected by indirect hemagglutination assay and 4 of 30 (13.3%) pus samples that showed bacterial infection by culture. The use of real-time PCR is a promising detection method for diagnosis and epidemiology assessment of amoebic liver abscess.

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“…Most of the other studies also had observed the similar laboratory findings. [23][24][25][26] In the present study pleuropulmonary complications were most common of which right side pleural effusion is most common, some cases of empyema also observed. Second most feared complication in our study is septicemia and shock followed by sub hepatic collection and peritonitis.…”

Section: Discussionmentioning

confidence: 99%

Clinicopathological study on presentation, diagnosis and management of liver abscess in Bhopal region

Siroliya¹,

Damor²,

Songra³

2017

Int Surg J

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Background: This prospective observational study is carried out to study cases of liver abscess and to determine demographic profile, spectrum of clinical presentations, aetiology, laboratory investigations. The objective of the study was to evaluate efficacy of Ultrasonographic (radiological) studies in determining the aetiology and in differentiating from other liver pathologies which may change the treatment outcome, bacteriological and serological characteristics, to study the influence of alcohol, diabetics and immunocompromised diseases (esp. HIV) leading to increased incidence of liver abscess and to evaluate efficacy, recurrence rate, complications, morbidity and mortality, duration of hospital stay associated with different management Strategies.Methods: This prospective observational study was carried out in Department of Surgery, Gandhi Medical College Bhopal and Associated Hamidia Hospital, Bhopal between July 2015 to October 2016.Results: Amoebic abscess (74%) is more common than pyogenic abscess (26%). Amoebic abscess is common in the age group of 31-50 years (73%), pyogenic in the age group of 51-70 years (73.1%). Male preponderance is found in case of amoebic liver abscess (90.5%). Right lobe involvement in common. Right upper quadrant pain, tenderness and fever are the most common clinical features. Alcoholism is most common risk factor (71.6%) and diabetes mellitus has strong association with pyogenic liver abscess (15.4%). E coli (19.2%) and klebsiella (11.5%) are the most common organisms cultured. Medical therapy is more useful in case of amoebic liver abscess (58.1%) while catheter drainage is more useful in case of pyogenic liver abscess (61.5%). Pleuropulmonary complications are much more common and complications rate is more common among pyogenic group.Conclusions: In our study, alcohol was found to be the most common predisposing factor for liver abscesses (68%), this underpin the finding of other studies. Amoebic liver abscess is a medically treated common infection prevailing in unhygienic condition, affecting people mostly between 30-40 years of age whereas pyogenic liver abscess patient commonly falls between 50-70 years age group. Both liver abscesses show a male preponderance. The present study also corroborates the catheter drainage procedure as a superior modality in treating pyogenic liver abscess.

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Invasive amebiasis: challenges in diagnosis in a non-endemic country (Kuwait).

Cited by 30 publications

References 11 publications

Laboratory Diagnostic Techniques for Entamoeba Species

Laboratory Diagnostic Techniques for Entamoeba Species

Application of Real-Time Polymerase Chain Reaction in Detection ofEntamoeba histolyticain Pus Aspirates of Liver Abscess Patients

Clinicopathological study on presentation, diagnosis and management of liver abscess in Bhopal region

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