Invasion of Human Oral Epithelial Cells by Prevotella intermedia

Abstract

Invasion of oral epithelial cells by pathogenic oral bacteria may represent an important virulence factor in the progression of periodontal disease. Here we report that a clinical isolate ofPrevotella intermedia, strain 17, was found to invade a human oral epithelial cell line (KB), whereas P. intermedia 27, another clinical isolate, and P. intermedia 25611, the type strain, were not found to invade the cell line. Invasion was quantified by the recovery of viable bacteria following a standard antibiotic protection assay and observed by electron microscopy. Cytochalasin D, cycloheximide, monodansylcadaverine, and low temperature (4°C) inhibited the internalization of P. intermedia 17. Antibodies raised against P. intermedia type C fimbriae and against whole cells inhibited invasion, but the anti-type-C-fimbria antibody inhibited invasion to a greater extent than the anti-whole-cell antibody. This work provides evidence that at least one strain ofP. intermedia can invade an oral epithelial cell line and that the type C fimbriae and a cytoskeletal rearrangement are required for this invasion.

Identification of intracellular oral species within human crevicular epithelial cells from subjects with chronic periodontitis by fluorescence in situ hybridization

“…These sites provide a constant source for recolonization and cross-infection within the mouth, which may result in persistence of periodontal infections and/or dissemination of bacteria to other host sites (25). In fact, P. gingivalis has been shown within endothelial cells and atherosclerotic plaque, supporting the evidence of association between periodontal disease and systemic infections (3,26). Regarding the specificity of the intracellular microbiota, no significant differences in the prevalence and numbers of internalized periodontopathogens were found between the subject groups.…”

“…In these processes, intracellular invasion of oral epithelial cells and gingival tissues is an important property of many oral species, including the periodontal pathogens Porphyromonas gingivalis, Treponema denticola, Tannerella forsythia, Actinobacillus actinomycetemcomitans, Fusobacterium nucleatum, and Prevotella spp. (2)(3)(4)(5)(6)(7)(8)(9)(10). Most of those studies, however, were performed in vitro, using few reference bacterial strains and either oral epithelial cell lines or primary epithelial cell or tissue cultures.…”

Does the Prevalence of Periodontal Pathogens Change in Elderly Edentulous Patients after Complete Denture Treatment?

“…This binding then induces internalization, which is essential for host cell invasion. 24 Oral hygiene is one of the most important factors that may influence the presence of oral bacteria. Patients who participated in the study were thoroughly instructed how to properly clean and store dentures, and were monitored by professionals.…”

Endotoxin Levels Are Associated With High‐Density Lipoprotein, Triglycerides, and Troponin in Patients With Acute Coronary Syndrome and Angina: Possible Contributions From Periodontal Sources

“…10 These bacterial species can invade periodontal epithelium and connective tissues, 11,12 can invade and persist within endothelial cells in culture, and were detected and shown to be viable in coronary endothelium and atherosclerotic plaques. [13][14][15][16][17][18][19] In vitro, P. gingivalis in the presence of LDL was shown to induce macrophages to become foam cells; 20 in vivo, the extent of periodontitis-affected tissue has been directly associated with LDL activation of macrophages. 21 Perhaps most supportive of the potential for these bacteria to play a role in systemic disease pathogenesis is the finding that P. gingivalis and A. actinomycetemcomitans have been recovered in human atherosclerotic plaques.…”