Invasion of HeLa Cells by Salmonella typhimurium: A Model for Study of Invasiveness of Salmonella

Giannella, Ralph A.; Washington, O.; Gemski, P.; Formal, Samuel B.

doi:10.1093/infdis/128.1.69

Cited by 176 publications

(116 citation statements)

References 14 publications

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“…However, the prolongation of the infection period gave positive results for Salmonella but not for these strains of Yersinia. This result agreed fairly well with that of the cell culture study on Salmonella by Giannella et al (2), and it was evident that Salmonella has affinity to HeLa cells although it is low.…”

Section: Discussionsupporting

confidence: 91%

Studies on the Pathogenicity ofYersinia enterocolitica

Une

1977

Microbiology and Immunology

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Analysis of the pathogenicity of Yersinia enterocolitica was performed by means of cell culture studies on the interaction of the organisms with HeLa cells and rabbit peritoneal macrophages based on observations of the pathogenic behavior of the organisms in vivo (11). The pathogenic strains, which successfully produced experimental enterocolitis in rabbits (11), had the ability to penetrate HeLa cells and to survive or multiply within the macrophages. The nonpathogenic strains, lacking the ability to produce pathological changes in rabbits (11), failed to penetrate HeLa cells, except for one strain, and also to survive totally or multiply within the macrophages. It was evident that the abilities of the organisms to penetrate epithelial linings which serve as the barrier of intestinal mucosa and to survive or multiply within the host cells appears to be closely related to the pathogenicity of Y. enterocolitica.

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Section: Discussionsupporting

confidence: 91%

Studies on the Pathogenicity ofYersinia enterocolitica

Une

1977

Microbiology and Immunology

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“…For most enteric pathogens attachment is a prerequisite for invasion and effective delivery of toxins to the intestinal epithelial cells (Giannella, 1981). Thus adhesiveness to cell cultures has been used as a measure of virulence; and since the invasion of tissue culture cells correlates with the capacity of enteropathogenic bacteria to invade intestinal epithelial cells in vivo (Giannella et al 1973), it is likely that the activity of these campylobacter strains towards the cell cultures is a reflexion of their pathogenicity.…”

Section: Colonization Of Infant Micementioning

confidence: 99%

The virulence of clinical and environmental isolates ofCampylobacter jejuni

Newell

McBride

Saunders

et al. 1985

J. Hyg.

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SUMMARYThe virulence of Campylobacter jejuni and C. coli isolated from various water sources was compared with that of clinical strains by in vitro assays of adhesion, invasion and cytotoxicity to HeLa cells. Variation in degree of attachment was observed, but this did not appear to be related to strain source, However, water strains were less invasive and less cytotoxic to HeLa cells than clinical strains as shown by immunofluorescence and electron microscopy.These differences were particularly evident between clinical and water isolates of the same serotype and biotype implicated in an outbreak of campylobacter enteritis in a school. The enhanced virulence of the clinical isolates, possibly induced by passage, was confirmed by colonization tests on infant mice.

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“…This entry process resembles phagocytosis and results in intracellular bacteria enclosed within membrane-bound vacuoles. To study the molecular details of the initial entry process, in vitro assays have been developed for quantitating the ability of Salmonella to adhere to and enter cultured mammalian cells (7)(8)(9)(10)(11)(12)(13). Recent studies (14)(15)(16) have examined the interaction of Salmonella with epithelial cells that have formed polarized monolayers in vitro (17).…”

mentioning

confidence: 99%

The ability of Salmonella to enter mammalian cells is affected by bacterial growth state.

Lee

Falkow

1990

Proc. Natl. Acad. Sci. U.S.A.

414

369

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We have examined the effect of different growth conditions on the ability of Salmonella to interact with Madin-Darby canine kidney cells. Two growth conditions that affect the expression of Salmonella adherence and invasiveness have been identified. First, bacteria lose their invasiveness in the stationary phase of growth. Second, bacteria growing in oxygen-limited growth conditions are induced for adherence and invasiveness, whereas those growing aerobically are relatively nonadherent and noninvasive. Salmonella from cultures aerated with gas mixtures containing 0% or 1% oxygen were 6-to 70-fold more adherent and invasive than those from cultures aerated with a gas mixture containing 20% oxygen.The Salmonella typhimurium oxrA gene that is required for the anaerobic induction of many proteins is n1 involved in the regulation of Salmonella invasiveness. We speculate that oxygen limitation might be an environmental cue that triggers the expression of Salmonella invasiveness within the intestinal lumen and other tissues.

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Invasion of HeLa Cells by Salmonella typhimurium: A Model for Study of Invasiveness of Salmonella

Cited by 176 publications

References 14 publications

Studies on the Pathogenicity ofYersinia enterocolitica

Studies on the Pathogenicity ofYersinia enterocolitica

The virulence of clinical and environmental isolates ofCampylobacter jejuni

The ability of Salmonella to enter mammalian cells is affected by bacterial growth state.

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