Inulin hydrolysis by inulinase immobilized covalently on magnetic nanoparticles prepared with wheat gluten hydrolysates

Torabizadeh, Homa; Mahmoudi, Asieh

doi:10.1016/j.btre.2018.02.004

Cited by 23 publications

(18 citation statements)

References 24 publications

(26 reference statements)

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“…On the other hand, for the immobilized enzyme, the yield of fructose was observed to be 18.2 g/L at 30°C which increased to 35.8 g/L at 60°C. This higher optimum temperature for immobilized inulinase could be explained by the increase in structural rigidity and decrease in flexibility of the covalently immobilized inulinase [47]. However, a further increase in the temperature led to a decrease in fructose yield and at 80°C; a fructose yield of 10.2 g/L and 20.4 g/L was obtained with the free and immobilized inulinase, respectively.…”

Section: Hydrolysis Of Inulinmentioning

confidence: 96%

Magnetically assisted commercially attractive chemo-enzymatic route for the production of 5-hydroxymethylfurfural from inulin

Saikia

Rathankumar

Varghese

et al. 2020

Biomass Conv. Bioref.

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Biomass has recently been extensively exploited as an attractive alternative to the scarce fossil fuels and has been widely used for the production of fine chemicals. The present work defines an integrated approach for high yield of 5-hydroxymethylfurfural (HMF) from inulin via a two-step process. Initially, insolubilization of inulinase from Aspergillus niger on chitosan-coated magnetic nanoparticles (cMNP) was achieved, and under optimal conditions, hydrolysis of 5% inulin by immobilized inulinase (2.5 U/mg) led to fructose release of 35.8 g/L at pH 6.0 and temperature 60°C in 3 h. Subsequently, the fructose was dehydrated to HMF in the presence of TiO 2 -magnetic silica spheres (TiO 2 -MSS). Upon optimizing the process parameters, HMF yield of 96.58% was achieved with 15% fructose in 15 min at 140°C with a TiO 2 -MSS load of 10% (w/w). Concerning the operational stability of the immobilized biocatalyst/catalyst, the immobilized inulinase was recycled up to 10 cycles of inulin hydrolysis with 9.2 g/L of fructose released at the 10th cycle. TiO 2 -MSS also showed high operational stability and were recycled up to 10 cycles of HMF production with 5.4 g/L of HMF produced at 10th cycle. The mass balance for inulin to HMF was performed which showed the efficiency of the process.

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Section: Hydrolysis Of Inulinmentioning

confidence: 96%

Magnetically assisted commercially attractive chemo-enzymatic route for the production of 5-hydroxymethylfurfural from inulin

Saikia

Rathankumar

Varghese

et al. 2020

Biomass Conv. Bioref.

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“…Therefore, thermostable inulinases are desirable biocatalysts for the chemical and food industry. In this context, Torabizadeh and Mahmoudi [25], covalently attached inulinase from Aspergillus niger onto wheat gluten hydrolysates (WGHs)-functionalized Fe 3 O 4 MNPs in the presence of glutaraldehyde as a cross-linking agent. As-developed inulinase was found to be active over a broader pH and temperature ranges, and also exhibited pronounced storage and thermal stability.…”

Section: Biotransformation Of Inulin To High Fructose Syrupmentioning

confidence: 99%

“…As-developed inulinase was found to be active over a broader pH and temperature ranges, and also exhibited pronounced storage and thermal stability. The inulinase showed 12.3 folds rise in enzyme half-life value following immobilization on MNPs at 75 • C and retained 70% of its preliminary catalytic activity after 12 continuous inulin hydrolysis cycles [25]. More recently, Mohammadi et al [23] fabricated unique biocompatible support i.e., glutathione-coated gold MNPs (GSH-AuMNPs) and used for the covalent immobilization of the inulinase enzyme (Figure 4).…”

Section: Biotransformation Of Inulin To High Fructose Syrupmentioning

confidence: 99%

“…75 °C and retained 70% of its preliminary catalytic activity after 12 continuous inulin hydrolysis cycles [25]. More recently, Mohammadi et al [23] fabricated unique biocompatible support i.e., glutathionecoated gold MNPs (GSH-AuMNPs) and used for the covalent immobilization of the inulinase enzyme (Figure 4).…”

Section: Biotransformation Of Inulin To High Fructose Syrupmentioning

confidence: 99%

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Bio-Catalysis and Biomedical Perspectives of Magnetic Nanoparticles as Versatile Carriers

et al. 2019

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In recent years, magnetic nanoparticles (MNPs) have gained increasing attention as versatile carriers because of their unique magnetic properties, biocatalytic functionalities, and capabilities to work at the cellular and molecular level of biological interactions. Moreover, owing to their exceptional functional properties, such as large surface area, large surface-to-volume ratio, and mobility and high mass transference, MNPs have been employed in several applications in different sectors such as supporting matrices for enzymes immobilization and controlled release of drugs in biomedicine. Unlike non-magnetic carriers, MNPs can be easily separated and recovered using an external magnetic field. In addition to their biocompatible microenvironment, the application of MNPs represents a remarkable green chemistry approach. Herein, we focused on state-of-the-art two majorly studied perspectives of MNPs as versatile carriers for (1) matrices for enzymes immobilization, and (2) matrices for controlled drug delivery. Specifically, from the applied perspectives of magnetic nanoparticles, a series of different applications with suitable examples are discussed in detail. The second half is focused on different metal-based magnetic nanoparticles and their exploitation for biomedical purposes.

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“…Previously, inulinase has been immobilized on various supports, e.g. biopolymers (chitin, chitosan, alginate) [16][17][18][19][20], synthetic polymers (polyurethane foam, polyvinyl alcohol) [21,22], inorganic supports (MWCNT, silica nanoparticles) [3,[23][24][25], or hybrids consisting of organic and inorganic parts (poly-d-lysine coated CaCO 3 microparticles, magnetic nanoparticles with wheat gluten hydrolysates, magnetite chitosan microparticles) [26][27][28]. Generally speaking, nanomaterials are currently widely used for immobilization of proteins.…”

Section: Introductionmentioning

confidence: 99%

Inulinase immobilization on polyethylene glycol/polypyrrole multiwall carbon nanotubes producing a catalyst with enhanced thermal and operational stability

Temkov

Petrovski

Gjorgieva

et al. 2019

Engineering in Life Sciences

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This paper describes the development of a simple method for mixed non‐covalent and covalent bonding of partially purified inulinase on functionalized multiwall carbon nanotubes (f‐MWCNTs) with polypyrrole (PPy). The pyrrole (Py) was electrochemically polymerized on MWCNTs in order to fabricate MWCNTs/PPy nanocomposite. Two multiple forms of enzyme were bound to N‐H functional groups from PPy and ‐COO− from activated MWCNTs to yield a stable MWCNTs/PPy/PEG immobilized preparation with increased thermal stability. Fourier transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM) were used to confirm functionalization of nanoparticles and immobilization of the enzyme. The immobilization yield of 85% and optimal enzyme load of 345 μg protein onto MWCNTs was obtained. The optimum reaction conditions and kinetic parameters were established using the UV‐Vis analytical assay. The best functional performance for prepared heterogeneous catalyst has been observed at pH 3.6 and 10, and at the temperatures of 60 and 80ºC. The half‐life (t1/2) of the immobilized inulinase at 60 and 80ºC was found to be 231 and 99 min, respectively. The reusability of the immobilized formulation was evaluated based on a method in which the enzyme retained 50% of its initial activity, which occurred after the eighteenth operation cycle.

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Inulin hydrolysis by inulinase immobilized covalently on magnetic nanoparticles prepared with wheat gluten hydrolysates

Abstract: Graphical abstract

Cited by 23 publications

References 24 publications

Magnetically assisted commercially attractive chemo-enzymatic route for the production of 5-hydroxymethylfurfural from inulin

Magnetically assisted commercially attractive chemo-enzymatic route for the production of 5-hydroxymethylfurfural from inulin

Bio-Catalysis and Biomedical Perspectives of Magnetic Nanoparticles as Versatile Carriers

Inulinase immobilization on polyethylene glycol/polypyrrole multiwall carbon nanotubes producing a catalyst with enhanced thermal and operational stability

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