Inulin Diet Intervention on Chemopreventive and Inflammatory Markers in Tumorigenesis of Colorectal Cancer

Mast cell neoplasia in dogs can occur in two different forms: common as cutaneous tumor, or less common as a systemic form of neoplastic mast cell proliferation -systemic mastocytosis. The aim of this study was to compare the histological and cytological features, KIT receptor expression and presence of c-KIT proto-oncogene mutations in neoplastic cells of dogs with canine cutaneous mast cell tumor (CMCT) and systemic mastocytosis. Microscopical examination of the cytological smears obtained from all selected dogs revealed that cellular specimens were constituted mostly of round cells with a central nuclei and fi ne to coarse purple cytoplasmic granules. Histopathological examination of skin samples of dogs with CMCT and a dog with systemic mastocytosis showed proliferation of the neoplastic mast cells in the superfi cial and/or deep dermis. Similar fi ndings were observed in tissue samples derived from lymph nodes, spleen, liver, myocardium and kidneys of a dog with systemic mastocytosis. Three dogs with high grade CMCT as well as one dog with systemic mastocytosis showed cytoplasmic CD117 expression, while 3 dogs with low grade CMCT, had membranous expression of CD117. Based on our study, histological features and cytoplasmic CD117 expression in neoplastic cells of dogs with systemic mastocytosis are similar to those in dogs with high grade CMCTs. Nevertheless, mutations of c-KIT proto-oncogene were not found in tumor samples either from dogs with CMCT or dog with systemic mastocytosis.

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Use Of The Prebiotic Inulin In The Prevention Of Adverse Signs Of Acute Colitis

Hijová

Šoltésová

Salaj

et al. 2015

Acta Veterinaria

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The aim of this study was to investigate the infl uence of prebiotic inulin diet intervention on the activity of β-glucuronidase and counts of coliforms and lactobacilli in fresh caecal digesta, cytokine levels (IL-6, IL-8) and transcription nuclear factor kappa beta (NFkB) activities in the colon tissue and blood samples of rats with dextran sulphate sodium (DSS) induced acute colitis. Male Sprague-Dawley rats (8 per group) were randomly divided into three groups: Control, Acute colitis and Prebiotic. Colitis was induced using 5% DSS in drinking water for 7d. DSS application signifi cantly increased the activity of β-glucuronidase (p<0.001), increased counts of coliform bacteria and decreased lactobacilli count (p<0.05) in comparison to the control group. Serum and tissue levels of IL-6 and IL-8 as well as tissue NFkB activities showed an increased expression in the acute colitis group. These results correspond to the average value of the disease activity index score (DAI) and revealed the maximum DAI score (6.5) in the acute colitis group. A decrease in the DAI score (4.13) was observed after application of the prebiotic inulin. Inulin diet intervention positively modifi ed the number of microorganims and decreased β-glucuronidase activity. Colon tissue activities of NFkB were signifi cantly suppressed (p<0.001). The synthesis of proinfl ammatory cytokines IL-6 (p<0.01) in the serum and in the colon tissue, as well as tissue in the prebiotic group were downregulated. These fi ndings indicate that the dietary intake of inulin suppressed the expression of the observed markers, which play an important role in the infl ammatory process, which predisposes to the use of inulin in the prevention or treatment of acute colitis in human and veterinary medicine.

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Inulin Diet Intervention on Chemopreventive and Inflammatory Markers in Tumorigenesis of Colorectal Cancer

Cited by 5 publications

References 10 publications

Intestinal-Based Diseases and Peripheral Infection Risk Associated with Gut Dysbiosis: Therapeutic use of Pre- and Probiotics and Fecal Microbiota Transplantation

Intestinal-Based Diseases and Peripheral Infection Risk Associated with Gut Dysbiosis: Therapeutic use of Pre- and Probiotics and Fecal Microbiota Transplantation

Morphological Features and KIT Receptor Expression in Canine Cutaneous Mast Cell Tumor and Systemic Mastocytosis / Morfološke Karakteristike I Ekspresija KIT Receptora Kod Kutanih Mastocitoma I Sistemske Mastocitoze Pasa

Use Of The Prebiotic Inulin In The Prevention Of Adverse Signs Of Acute Colitis

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