Introgression of virus-resistance genes into traditional Spanish tomato cultivars ( Solanum lycopersicum L.): Effects on yield and quality

Rubio, Fernando; Alonso, Aránzazu; García‐Martínez, Santiago; Martínez, Juan José Ruiz

doi:10.1016/j.scienta.2015.11.025

Cited by 17 publications

(13 citation statements)

References 22 publications

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“…Therefore, several backcrosses must be carried out to recover a major proportion of the commercial traits. This situation has been documented by Rubio et al (2016), who evaluated the yield and fruit quality in a population of near isogenic lines and found that the total and commercial yield decreased by 50%. In the present study, the crosses derived between native and commercial lines did not show a significant loss concerning to firmness, yield and fruit size.…”

Section: Agronomic Performance Of Hybridsmentioning

confidence: 72%

“…In plant breeding, the introduction of exotic germplasm has played an important role for obtaining new hybrids with one novel trait; e.g, pest resistance, salt stress tolerance and increased yield (Cox et al, 1984;Hanson et al, 2000;Frary et al, 2011;Lange et al, 2011;Yang et al, 2012;Gaikwad et al, 2014;Rubio et al, 2016). In tomato, many studies have classified several lines on the basis of their combing ability (Gul et al, 2010;Chattopadhyay et al, 2011;El-Gabrya et al, 2014).…”

Section: General and Specific Combining Ability Effectsmentioning

confidence: 99%

See 1 more Smart Citation

Exploring the breeding potential of Mexican tomato landraces

Martínez-Vázquez

Hernández-Bautista

Lobato-Ortíz

et al. 2017

Scientia Horticulturae

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Section: Agronomic Performance Of Hybridsmentioning

confidence: 72%

Section: General and Specific Combining Ability Effectsmentioning

confidence: 99%

Exploring the breeding potential of Mexican tomato landraces

Martínez-Vázquez

Hernández-Bautista

Lobato-Ortíz

et al. 2017

Scientia Horticulturae

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“…Despite the breeding program's success, homozygous alleles for resistance, especially for the Ty-1 gene, have been found to negatively affect both production levels and other parameters of agronomic quality [43]. This work does not clarify whether the negative effects on production and quality are directly due to the Ty-1 gene, or whether they are caused by genes associated with the resistance gene.…”

Section: Obtaining Plants With the Ty-1 Gene With Less Linkage Dragmentioning

confidence: 87%

“…This decrease in production is due to the introgressed genes themselves and/or to the linkage drag associated with the resistance genes, particularly the Ty-1 gene. This problem has been previously described in tomatoes for industrial use [41], in tobacco [42], and in tomatoes for fresh consumption [43]. In an attempt to avoid these problems, we have developed lines that are not resistant to TYLCV.…”

Section: Lines Obtained In the Epso-umh Breeding Programmentioning

confidence: 97%

Twenty Years of Tomato Breeding at EPSO-UMH: Transfer Resistance from Wild Types to Local Landraces—From the First Molecular Markers to Genotyping by Sequencing (GBS)

et al. 2018

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In 1998, the plant breeding team at the School of Engineering of Orihuela (EPSO), part of the Miguel Hernández University (UMH) in Elche, commenced a tomato breeding program. Marker-assisted selection and backcrossing were used to simultaneously introduce three genes (Tm-2a, Ty-1, and Sw-5) that confer resistance to relevant viruses, such as tomato mosaic virus (ToMV), tomato yellow curl virus (TYLCV), and tomato spotted wilt virus (TSWV), to traditional varieties of local tomatoes, specifically the "Muchamiel" and the "De la pera" types. After each backcross, cleaved amplified polymorphic sequence (CAPS) molecular markers were used to select the plants with the resistance genes of interest. A previously described marker was used for TSWV, and new markers were designed for ToMV, and TYLCV using available sequences in the National Center for Biotechnology Information (NCBI) database. In parallel to the breeding program, several molecular markers-Sequence Related Amplified Polymorphism (SRAP), Simple Sequence Repeats (SSRs), Amplified Fragment Length Polymorphisms (AFLPs), Single Nucleotide Polymorphisms (SNPs), and (GATA) 4 probes-were used to study genetic variability, and to identify a collection of Spanish and Italian traditional tomato varieties. The results showed a limited genetic variability among cultivated tomato varieties. The breeding lines Muchamiel UMH 1200, and De la pera 1203 (both with homozygous resistance to the three viruses) were the first new varieties that were obtained. They were included in the Register of Protected Plant Varieties in 2013. Lines without a resistance to TYLCV were also developed, and protected in 2017. We have begun to use SNP massive genotyping for studies of genetic association, and for selecting plants with the Ty-1 gene with less linkage drag. Molecular markers have been extremely useful in identifying the different steps of the tomato breeding program at EPSO-UMH.

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“…A pesar del éxito del programa, en los últimos años se ha venido observando que la presencia de los genes de resistencia (en particular la del gen Ty-1) está afectando negativamente tanto al rendimiento como a otros parámetros agronómicos y de calidad del fruto en las líneas UMH 3…”

Section: Introductionunclassified

Programa de mejora genética de variedades tradicionales de tomate en la Universidad Miguel Hernández de Elche: pasado, presente y futuro

Carbonell

2018

DOCTUMH

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Las variedades tradicionales de tomate De la Pera y Muchamiel son muy apreciadas en el sureste español. Sin embargo, su cultivo se está perdiendo debido a su gran susceptibilidad a las virosis, lo que puede suponer una grave pérdida de biodiversidad. En 1998, el grupo de genética vegetal de la Universidad Miguel Hernández de Elche inició un programa de mejora con el objetivo de introducir genes dominantes de resistencia a las tres virosis más importantes que afectan al tomate en estas dos variedades. Tras diecinueve años de mejora, se han obtenido cinco líneas de tipo Muchamiel y otras cinco de De la Pera, con diversos genotipos de resistencia y calidad agronómica y organoléptica aceptables. A pesar del éxito, se ha comprobado que las líneas mejoradas que presentan los tres genes de resistencia tienen una producción y una calidad menor que los parentales originales. Todo apunta al efecto negativo que produce el gen Ty-1 (confiere tolerancia a TYLCV) en homocigosis, o bien los genes asociados al mismo. Por ello, actualmente se está cuantificando el efecto de la introgresión de estos genes, y elaborando un proyecto de búsqueda de resistencias que no afecten al rendimiento o a cualquier otro parámetro de calidad de las líneas UMH.

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Introgression of virus-resistance genes into traditional Spanish tomato cultivars ( Solanum lycopersicum L.): Effects on yield and quality

Cited by 17 publications

References 22 publications

Exploring the breeding potential of Mexican tomato landraces

Exploring the breeding potential of Mexican tomato landraces

Twenty Years of Tomato Breeding at EPSO-UMH: Transfer Resistance from Wild Types to Local Landraces—From the First Molecular Markers to Genotyping by Sequencing (GBS)

Programa de mejora genética de variedades tradicionales de tomate en la Universidad Miguel Hernández de Elche: pasado, presente y futuro

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