Introgression of the rice blast resistance genesPi1, Pi2andPi33into Russian rice varieties by marker-assisted selection

Abstract

Marker-assisted selection (MAS) was adopted for introgression of broad-spectrum blast resistance genes Pi1, Pi2 and Pi33 into elite Russian rice varieties. It was shown that microsatellite markers RM 224, RM 527 and RM 310 may be effectively used to transfer Pi1, Pi2 and Pi33 genes into selected Russian genotypes of rice. Based on the highly productive variety Kuboyar, we obtained the lines, 200-5668 (Pi 1+2+33 IL14 × Kuboyar), 207-5671 (Pi 1+2+33 IL28 × Kuboyar) and 208-5674 (Pi 1+2+33 IL28 × Kuboyar) carrying three pyramided genes Pi1, Pi2 and Pi33 in homozygous state. The lines 200-5668, 207-5671 and 208-5674 are used as an improved resistance donor source to obtain hybrids and pyramiding additional Pi genes in order to provide stable long-term resistance for rice blast disease.

INHERITANCE OF BLAST DISEASE RESISTANCE IN THE CROSS HUR 3022 X TETEP OF RICE (Oryza sativa L.)

“…Although, chemicals can control blast but use of blast-resistant cultivars in rice production is considered an economical and environment friendly disease control strategy. Out of 100 blast resistant genes till identified, several resistance genes have been already introduced into elite rice varieties using Marker Assisted Breeding approaches (Abhilash Kumar et al, 2016;Ellur et al, 2016;Sabin et al, 2016;Usatov et al, 2016;Khan et al, 2018;Kumar et al, 2019;Swathi et al, 2019). Dynamic changes in the race composition of pathogen has often caused breakdown of resistance in most of the improved resistant varieties.…”

Improving blast resistance of maintainer line DRR 9B by transferring broad spectrum resistance gene Pi2 by marker assisted selection in rice

“…The blast susceptible rice variety MR 263 was improved for blast resistance through MABC approach based on only foreground and phenotypic selection by Nihad et al ( 2022 ). The PCR-based DNA marker RM527 is tightly linked with Pi2 gene (Usatov et al 2016 ), was able to identify positive plants precisely without any false positive detection at any stage of MAS. While improving the maintainer line, DRR 9B for blast resistance and to be a perfect maintainer, it is compulsory that the backcross derived lines should not possess the major fertility restorer Rf4 gene.…”

“…The genomic DNA was isolated from donor, recurrent parent, and also from different generation breeding material by following the protocol reported by Dellaporta et al ( 1983 ). The PCR-based, SSR marker RM527 (Jiang et al 2015 ; Usatov et al 2016 ) was utilized in marker-assisted selection to confirm the presence of the Pi2 in the F 1 generation and subsequent backcross generations. The SSR markers RM6100 (Singh et al 2005 ) is closely linked to major fertility restorer gene Rf4 was utilized to identify plants possessing the non-fertility-restoring allele (i.e., rf4rf4 ) in homozygous condition (Table 1 ).…”

SSR Analysis of the Chloroplast and Mitochondrial Genomes of Cultivar Lines and Wild Types of the Sunflower (Helianthus L.)

“…As genomes of chloroplasts and mitochondria do not undergo sexual recombination are a good tool for studying accumulated differences at them. The contribution of the cytoplasm to the adaptive potential of plants to environmental factors has a greater impact than previously thought (Mashkina et al, 2010;Usatov et al, 2016;Sobanski et al, 2019). Generally, for cultivar lines characterized lower level of polymorphism of the chloroplast genome relative wild type forms (Wills et al, 2005;Singh et al, 2015).…”