Introgression of Aegilops mutica genes into common wheat genome

Єфіменко, Тетяна; Аntonyuk, Maksym; Martynenko, V. S.; Navalihina, Anastasiia; Ternovska, Tamara

doi:10.3103/s0095452718010048

Cited by 10 publications

(12 citation statements)

References 19 publications

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“…Інтрогресія генетичного матеріалу від дикорослих родичів до геному м'якої пшениці залишається актуальною, оскільки являє собою природне та невичерпне джерело збагачення генофонду пшениці за генами, що поліпшують її адаптивний потенціал (Iefimenko et al, 2018). У вітчизняній та світовій практиці існує багато прикладів успішного використання різних екотипів спельти в селекції та наступній гібридизації з голозерними пшеницями з метою поліпшення останніх і надання їм стійкості до абіотичних та біотичних стресорів, підвищення якісних показників зерна (Golik et al, 2016).…”

Section: використання спельти у селекційній роботіunclassified

Triticum spelta: Origin, biological characteristics and perspectives for use in breeding and agriculture

Бабенко

Hospodarenko

Rozhkov

et al. 2018

Regul. Mech. Biosyst.

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In today’s wheat production, the trends focused on the revival, selection and implementation of forgotten regional grain crops, the so-called "antique" cereals, have been clearly identified. One of these is the ancient hexaploid species of wheat – spelt (Triticum spelta). One of the main reasons that have contributed to the revival of this type of wheat in recent years is its tolerance to growing conditions and capacity to endure the natural and climatic factors of the environment, which allows it to be grown without an excessive use of plant protection products. It is precisely because of this feature that hulled wheat is referred to as "environmentally friendly”. Spelt is capable of yielding stable crops without strict adherence to the production technology and may be a great alternative to soft wheat. The growth in demand for this crop is also due to a complex of economic and valuable traits inherited from its wild ancestors. This review analyzes and summarizes the information about the origin of spelt, presents biological characteristics, discusses the perspectives for using the species in breeding work when implementing interspecific hybridization and compares the structure and genome evolution. The achievements on the revival of spelt as an independent species and its use as a source of economic and valuable traits in breeding programs of crosses with soft wheat are highlighted, examples of successful use of the economic and valuable traits of spelt for the improvement and creation of new high-quality wheat varieties are presented. Data on this superiority of grain from this species over that of genetically close wheat species are presented. The varietals’ characteristics for spelt varieties of Ukrainian selection are given. Such important spelt characteristics as resistance to biotic and abiotic stressors, high quality grain composition and yield have been analyzed.

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Section: використання спельти у селекційній роботіunclassified

Triticum spelta: Origin, biological characteristics and perspectives for use in breeding and agriculture

Бабенко

Hospodarenko

Rozhkov

et al. 2018

Regul. Mech. Biosyst.

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“…У роботі було використано такий рослинний матеріал: озиму м'яку пшеницю Triticum aestivum (2n=6x=42, AABBDD), cорт Аврора; геномно-заміщений амфідиплоїд Авротіка (2n=6x=42, AABBTT), який об'єднує тетраплоїдний компонент м'якої пшениці (AABB) та геном TT Amblyopyrum muticum; інтрогресивні лінії, отримані від схрещувань Авротіки та Аврори, що розщепилися із утворенням ліній, які мають різний фенотип: Tica 30.2/7 (Н25, Н31, Н32), Tica 42.4/8 (Н20, Н29) та Tica 14.4/1 (Н9, Н30, Н7, Н28) [15,16]. Батьківські генотипи Аврора та Авротіка є безостими, рослини ліній Н25, Н31, Н32, Н20, Н29 мають остеподібні відростки, рослини Н9, Н30, Н7, Н28 безості.…”

Section: матеріали і методиunclassified

Non-additive expression of awn development regulatory genes in the bread wheat lines with introgressions from Amblyopyrum muticum

Наваліхіна¹,

Антонюк²,

Ternovska³

2019

Fakt. eksp. evol. org.

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Aim. Nonadditive expression of homeotic genes is considered to cause the development of nonparental phenotypes in the plants of hybrid origin. Previously, orthologs of rice and barley awn development regulators TaTOB1, TaDL, TaKNOX3, and TaETT2 were identified in the bread wheat genome sequence. Nonadditive expression of these regulators can be the reason for the emergence of non-parental terminally awned phenotypes among the bread wheat lines with introgressions from Amblyopyrum muticum Methods. Gene expression was identified with end-point detection RT-qPCR Results. Introgressive lines have expression of TaTOB1, TaKNOX3, and TaETT2 at the lower level compared to parents. As orthologs of TaTOB1 and TaKNOX3 are negative regulators of awn development in rice and barley, their reduced expression could have caused the appearance of terminally awned plants among introgressive lines. Neverthless, the reduced expression of the genes wasn’t specific to the lines with non-parental phenotype. Conclusions. Due to the lack of correlation between reduced expression of the genes studied and non-parental phenotype of the introgressive lines, the role of nonadditive expression of TaTOB1, TaKNOX3, and TaETT2 in the development of this phenotype is not clear. Keywords: amphidiploids, non-additive expression, developmental genetics, awns.

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“…Інтрогресивні лінії, що походять від схрещування пшениці м'якої сорту Аврора (рекуррентний генотип) та амфідиплоїда Авротіка (Triticum aestivum/Amblyopyrum muticum) є цитологічно стабільними [17]. Втім, у їхньому геномі відбуваються генетичні та епігенетичні зміни, наслідки яких відображені у змінах їхнього фенотипу, зокрема таких ознак морфології колоса як колір та опушення луски.…”

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The role of siRNAs in genome stability maintaining in the bread wheat introgression lines

Наваліхіна¹,

Аntonyuk²,

Ternovska³

2020

Fakt. eksp. evol. org.

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Aim. Deviations of the siRNAs levels from the parental ones in the plants with hybrid genomes are associated with the activation of transposable elements (TE). This, in turn, lead to the further genome rearrangements. Introgression lines Triticum aestivum / Amblyopyrum muticum are cytologically stable, however, there are visible signs of genetic and (or) epigenetic restructuring that are still going on. Molecular mechanisms of these processes are the subject of our study. Methods. The levels of siRNAs in the plant lemmas were determined by small RNA-seq. Reads of the small RNA libraries were aligned to the repeats to find siRNA sequences. Results. Introgression lines (ILs) and parental amphidiploid have variable levels of siRNAs regulating MITE and CACTA transposable elements, compared with the parental bread wheat variety. For twelve TE sequences, majority of which are CACTA elements, decrease in the levels of siRNAs in ILs and amphidiploid, compared to the wheat, is statistically significant. Decreased siRNAs levels could lead to the activation of corresponding TE classes. Conclusions. Variation of siRNA levels in ILs and amphidiploid can be the key factor that cause rearrangements in their genomes. These include activation of TEs, changes in DNA methylation patterns, and gene expression variation. Therefore, detected changes in siRNA levels can be the molecular mechanisms of the processes that occur in studied hybrid genomes. Keywords: siRNA, transposable elements, amphidiploid, introgression lines.

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Introgression of Aegilops mutica genes into common wheat genome

Cited by 10 publications

References 19 publications

Triticum spelta: Origin, biological characteristics and perspectives for use in breeding and agriculture

Triticum spelta: Origin, biological characteristics and perspectives for use in breeding and agriculture

Non-additive expression of awn development regulatory genes in the bread wheat lines with introgressions from Amblyopyrum muticum

The role of siRNAs in genome stability maintaining in the bread wheat introgression lines

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