Introgression of a novel Thinopyrum intermedium St-chromosome-specific HMW-GS gene into wheat

Guang-rong, LI; Liu, Cheng; Li, Chenghui; Zhao, Jing; Zhou, Li; Dai, Gang Shu; Yang, Ennian; Yang, Zujun

doi:10.1007/s11032-013-9838-8

Cited by 28 publications

(20 citation statements)

References 42 publications

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“…uniaristata , Th. intermedium [Li et al, 2013], and Lophopyrum elongatum [Hu et al, 2012]. However, markers specific for Ae.…”

Section: Discussionmentioning

confidence: 99%

Molecular and Cytogenetic Characterization of a Powdery Mildew-Resistant Wheat-Aegilops mutica Partial Amphiploid and Addition Line

Liu

Li²,

Gong³

et al. 2015

Cytogenet Genome Res

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Aegilops mutica Boiss., a diploid species (2n = 2x = 14, TT), has been rarely studied before. In this research, a hexaploid wheat (cv. Chinese Spring)-Ae. mutica partial amphiploid and a wheat-Ae. mutica addition line were characterized by chromosome karyotyping, FISH using oligonucleotides Oligo-pTa535-1, Oligo-pSc119.2-1, and (GAA)₈ as probes, and EST-based molecular markers. The results showed that the partial amphiploid strain consisted of 20 pairs of wheat chromosomes and 7 pairs of Ae. mutica chromosomes, with both wheat 7B chromosomes missing. EST-based molecular marker data suggested that the wheat-Ae. mutica addition line carries the 7T chromosome. Resistance tests indicated that both the partial amphiploid and the 7T addition line were highly resistant to powdery mildew, whereas the wheat control line Chinese Spring was highly susceptible, indicating the presence of a potentially new powdery mildew resistance gene on the Ae. mutica 7T chromosome. The karyotype, FISH patterns, and molecular markers can now be used to identify Ae. mutica chromatin in a wheat background, and the 7T addition could be used as a new powdery mildew resistance source for wheat breeding.

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“…uniaristata , Th. intermedium [Li et al, 2013], and Lophopyrum elongatum [Hu et al, 2012]. However, markers specific for Ae.…”

Section: Discussionmentioning

confidence: 99%

Molecular and Cytogenetic Characterization of a Powdery Mildew-Resistant Wheat-Aegilops mutica Partial Amphiploid and Addition Line

Liu

Li²,

Gong³

et al. 2015

Cytogenet Genome Res

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“…In this study, we successfully transferred Th. intermedium chromosome 1St#2 to wheat and subsequently produced substitution and translocation lines (Hu et al 2011;Li et al 2013Li et al , 2015a which enabled us to determine the effect of 1St#2 chromosomes in a wheat background. Our results suggest that the Th.…”

Section: Discussionmentioning

confidence: 99%

“…trichophorum partial amphiploid TE-3 (Yang et al 2006) was used as the donor in the transfer of Thinopyrum chromosomes to wheat. Line AS1677 (2n = 42) contained a pair of Thinopyrum chromosomes as a 1St#2 (1D) substitution line which was developed from the crosses of wheat cultivar ML-13 to the partial amphiploid TE-3 (Hu et al 2011;Li et al 2013). Translocation lines E9-2 (2n = 42, T1DL.1St#2S) and E1233 (2n = 42, T1DS.1St#2L) were developed from crosses between AS1677 with the wheat cultivar MY11 .…”

Section: Plant Materialsmentioning

confidence: 99%

“…intermedium ssp. trichophorum chromosome 1St#2 substitution and translocation lines, which contained novel agronomically important genes (Hu et al 2011;Li et al 2013Li et al , 2015a. With the aim to precisely trace the 1St#2 chromosomes by marker assisted selection in wheat breeding programs for improving grain quality, the SLAF-seq technology was applied to wheat-Th.…”

Section: Introductionmentioning

confidence: 99%

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New molecular markers and cytogenetic probes enable chromosome identification of wheat-Thinopyrum intermedium introgression lines for improving protein and gluten contents

Guang-rong

Wang

Lang

et al. 2016

Planta

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New molecular markers were developed for targeting Thinopyrum intermedium 1St#2 chromosome, and novel FISH probe representing the terminal repeats was produced for identification of Thinopyrum chromosomes. Thinopyrum intermedium has been used as a valuable resource for improving the disease resistance and yield potential of wheat. A wheat-Th. intermedium ssp. trichophorum chromosome 1St#2 substitution and translocation has displayed superior grain protein and wet gluten content. With the aim to develop a number of chromosome 1St#2 specific molecular and cytogenetic markers, a high throughput, low-cost specific-locus amplified fragment sequencing (SLAF-seq) technology was used to compare the sequences between a wheat-Thinopyrum 1St#2 (1D) substitution and the related species Pseudoroegneria spicata (St genome, 2n = 14). A total of 5142 polymorphic fragments were analyzed and 359 different SLAF markers for 1St#2 were predicted. Thirty-seven specific molecular markers were validated by PCR from 50 randomly selected SLAFs. Meanwhile, the distribution of transposable elements (TEs) at the family level between wheat and St genomes was compared using the SLAFs. A new oligo-nucleotide probe named Oligo-pSt122 from high SLAF reads was produced for fluorescence in situ hybridization (FISH), and was observed to hybridize to the terminal region of 1St#L and also onto the terminal heterochromatic region of Th. intermedium genomes. The genome-wide markers and repetitive based probe Oligo-pSt122 will be valuable for identifying Thinopyrum chromosome segments in wheat backgrounds.

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“…Філогенетичний аналіз послідовності гена пирію вказує на те, що еволюційно він давніший, ніж гомологічні гени HMW-GS пшениці. Інтрогресія гена HMW-GS пирія у геном пшениці може впливати на збільшення врожайності та якості пшениці [2,5,6].…”

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Polymorphism in the sequence of Glu1 gene in populations of Thinopyrum intermedium as a possible adaptive trait

Аntonyuk¹,

Єфіменко²,

Ternovska³

2018

Fakt. eksp. evol. org.

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Aim. Compare structures of Glu genes, extracted from Th. intermedium plants grown in different microgeographic conditions. Methods. PCR with DNA samples of Th. intermedium and primers to Glu-1Dx and Glu-1Dy gene regions, sequencing and comparative analysis of polymorphic amplicons and sequences from databases. Results. Central fragments of Glu-1Dx and Glu-1Dy from plants grown in different microgeographic conditions had significant differences compared to corresponding sequences of Aegilops tauschii (Glu-1Dx), Thinopyrum intermedium (Glu-1St*2x), Triticum aestivum (Glu-1Dy) obtained from databases. Both genes had point mutations, deletions and insertions, which transformed glutamine coding triplets characteristic for storage proteins to stop-codons, also codons for cysteine, methionine and proline appeared. These aminoacids affect protein surface charge, hydrophilic/hydrophobic properties, conformation density, and structure stability. Conclusions. Changes in high molecular weight glutenin structure, which were caused by the observed mutations, could affect protease ability to effectively hydrolases them, which consequently could affect seed germination rate. Slow seed germination might be one of the mechanisms for survival of wild cereal species Thinopyrum intermedium under unfavorable environmental conditions.Keywords: Thinopyrum intermedium, Glu-1Dх, Glu-1Dy, sequence, point mutations, stop codon.

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Introgression of a novel Thinopyrum intermedium St-chromosome-specific HMW-GS gene into wheat

Cited by 28 publications

References 42 publications

Molecular and Cytogenetic Characterization of a Powdery Mildew-Resistant Wheat-<b><i>Aegilops mutica</i></b> Partial Amphiploid and Addition Line

Molecular and Cytogenetic Characterization of a Powdery Mildew-Resistant Wheat-<b><i>Aegilops mutica</i></b> Partial Amphiploid and Addition Line

New molecular markers and cytogenetic probes enable chromosome identification of wheat-Thinopyrum intermedium introgression lines for improving protein and gluten contents

Polymorphism in the sequence of Glu1 gene in populations of Thinopyrum intermedium as a possible adaptive trait

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