Introgression of a novel salt‐tolerant L‐myo‐inositol 1‐phosphate synthase from Porteresia coarctata (Roxb.) Tateoka (PcINO1) confers salt tolerance to evolutionary diverse organisms

Das-Chatterjee, Aparajita; Goswami, Lily; Maitra, Susmita; Dastidar, Krishnarup Ghosh; Ray, Sudipta; Majumder, Arun Lahiri

doi:10.1016/j.febslet.2006.06.033

Cited by 118 publications

(79 citation statements)

References 34 publications

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“…For example, transgenic poplar overaccumulating mannitol displayed increased salt tolerance in comparison with wild-type poplar (Hu et al, 2005). Myoinositol has also been implicated in salt tolerance (Majumder et al, 2003;Das-Chatterjee et al, 2006). Its biosynthesis was increased during salinity stress in common ice plant (Mesembryanthemum crystallinum; Ishitani et al, 1996) and in the Arabidopsis-related halophytic salt cress (Thellungiella halophila; Taji et al, 2004).…”

Section: Ems Lead To Activation Of Defenses and Prime Roots Formentioning

confidence: 97%

Upgrading Root Physiology for Stress Tolerance by Ectomycorrhizas: Insights from Metabolite and Transcriptional Profiling into Reprogramming for Stress Anticipation

Luo

Janz²,

Jiang³

et al. 2009

Plant Physiology

189

145

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Ectomycorrhizas (EMs) alleviate stress tolerance of host plants, but the underlying molecular mechanisms are unknown. To elucidate the basis of EM-induced physiological changes and their involvement in stress adaptation, we investigated metabolic and transcriptional profiles in EM and non-EM roots of gray poplar (Populus 3 canescens) in the presence and absence of osmotic stress imposed by excess salinity. Colonization with the ectomycorrhizal fungus Paxillus involutus increased root cell volumes, a response associated with carbohydrate accumulation. The stress-related hormones abscisic acid and salicylic acid were increased, whereas jasmonic acid and auxin were decreased in EM compared with non-EM roots. Auxin-responsive reporter plants showed that auxin decreased in the vascular system. The phytohormone changes in EMs are in contrast to those in arbuscular mycorrhizas, suggesting that EMs and arbuscular mycorrhizas recruit different signaling pathways to influence plant stress responses. Transcriptome analyses on a whole genome poplar microarray revealed activation of genes related to abiotic and biotic stress responses as well as of genes involved in vesicle trafficking and suppression of auxin-related pathways. Comparative transcriptome analysis indicated EM-related genes whose transcript abundances were independent of salt stress and a set of salt stress-related genes that were common to EM non-salt-stressed and non-EM salt-stressed plants. Salt-exposed EM roots showed stronger accumulation of myoinositol, abscisic acid, and salicylic acid and higher K + -to-Na + ratio than stressed non-EM roots. In conclusion, EMs activated stress-related genes and signaling pathways, apparently leading to priming of pathways conferring abiotic stress tolerance.Under natural conditions, many economically important tree species including fast-growing poplars (Populus spp.) form ectomycorrhizas (EMs) between roots and EM fungi. Colonization with EM fungi leads to profound changes in root architecture and morphology. Usually, EM roots are strongly ramnified and EM root tips show a bulb-like appearance (Smith and Read, 2008). In EMs, plants and fungi interact mutualistically: while the plant receives mineral nutrients and water through the fungus, the fungus is supplied with carbohydrates by its host (Smith and Read, 2008). To fulfill these functions, specific anatomical structures are established, which involve the formation of a hyphal mantle ensheathing the root tip with hyphae emanating into soil for nutrient uptake. Inside the mantle, hyphae grow between, but not inside, the cortex cells within the cell wall, forming an interface called the Hartig net for bidirectional nutrient exchange.The establishment of EMs requires a coordinated developmental program in both partners of the symbiosis. Transcriptional changes during initial stages of host recognition and colonization have been the focus of several recent studies (Johansson et al., 2004;Duplessis et al., 2005;Le Queré et al., 2005;Morel et

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Section: Ems Lead To Activation Of Defenses and Prime Roots Formentioning

confidence: 97%

Upgrading Root Physiology for Stress Tolerance by Ectomycorrhizas: Insights from Metabolite and Transcriptional Profiling into Reprogramming for Stress Anticipation

Luo

Janz²,

Jiang³

et al. 2009

Plant Physiology

189

145

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“…It participates in diverse array of biological processes like membrane biogenesis (Low and Saltiel 1988), phosphorus storage, signal transduction as second messenger (Biswas et al 1984) and abiotic-stress tolerance in higher plants (Bohnert et al 1995;Nelson et al 1998;Majee et al 2004;Das-Chatterjee et al 2006). The synthesis of myoinositol involves a highly conserved two-step process that is common in all organisms studied so far.…”

Section: Introductionmentioning

confidence: 97%

Functional identification of sll1383 from Synechocystis sp PCC 6803 as L-myo-inositol 1-phosphate phosphatase (EC 3.1.3.25): molecular cloning, expression and characterization

Patra

Dastidar

Maitra

et al. 2006

Planta

Self Cite

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The genome sequence of the cyanobacterium Synechocystis sp. PCC6803 revealed four Open reading frame (ORF) encoding putative inositol monophosphatase or inositol monophosphatase-like proteins. One of the ORFs, sll1383, is approximately 870 base pair long and has been assigned as a probable myo-inositol 1 (or 4) monophosphatase (IMPase; EC 3.1.3.25). IMPase is the second enzyme in the inositol biosynthesis pathway and catalyses the conversion of L-myo-inositol 1-phosphate to free myo-inositol. The present work describes the functional assignment of ORF sll1383 as myo-inositol 1-phosphate phosphatase (IMPase) through molecular cloning, bacterial overexpression, purification and biochemical characterization of the gene product. Affinity (K (m)) of the recombinant protein for the substrate DL-myo-inositol 1-phosphate was found to be much higher (0.0034 +/- 0.0003 mM) compared to IMPase(s) from other sources but in comparison V (max) ( approximately 0.033 mumol Pi/min/mg protein) was low. Li(+) was found to be an inhibitor (IC(50) 6.0 mM) of this enzyme, other monovalent metal ions (e.g. Na(+), K(+) NH (4) (+) ) having no significant effect on the enzyme activity. Like other IMPase(s), the activity of this enzyme was found to be totally Mg(2+) dependent, which can be substituted partially by Mn(2+). However, unlike other IMPase(s), the enzyme is optimally active at approximately 42 degrees C. To the best of our knowledge, sll1383 encoded IMPase has the highest substrate affinity and specificity amongst the known examples from other prokaryotic sources. A possible application of this recombinant protein in the enzymatic coupled assay of L-myo-inositol 1-phosphate synthase (MIPS) is discussed.

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“…Majee et al ( 2004 ) isolated and characterized a novel salt-tolerant gene L-myo-inositol 1-phosphate synthase from halophytic rice, Porteresia coarctata . Incorporating this gene into rice and other plant species increased synthesis of inositol under salinity stress and enhanced tolerance to salinity stress (Das-Chatterjee et al 2006 ) . Jacobs et al ( 2011 ) identifi ed a sodium pumping ATPase gene PpENA1 from the moss Physcomitrella patens , which is not found in higher plants.…”

Section: Transformation In Crop Plantsmentioning

confidence: 99%

Approaches to Increasing Salt Tolerance in Crop Plants

Karan

Subudhi

2011

Abiotic Stress Responses in Plants

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Soil salinity is widely recognized as a major threat to global food security. Salinity and other abiotic stresses, which are expected to be more frequent in future due to disturbances in global climate, pose a serious challenge for plant scientists to ensure food supply for the growing world population. Several approaches have been advocated to address the salinity problem, but the most logical solution to maintain crop productivity in salt-affected areas is to enhance salt tolerance of crop plants. Due to the genetic and physiological complexity associated with salt tolerance, efforts to breed salt-tolerant plants have met with limited success. Although progress has been made in deciphering the genetic basis of salt tolerance, sustained efforts are needed to systematically dissect and utilize the natural variability in the available germplasm for improving crop adaptation in saline environments using modern genomics tools. Wide range of variability for salt tolerance in wild relatives, cultivars of major fi eld crops, and halophytes offers bright prospect for discovery of superior salt-tolerant alleles for crop improvement. With an enhanced understanding of molecular mechanisms and the associated genes for component traits of salt tolerance, it would be possible to breed salt-tolerant plants using an integrated approach involving conventional breeding, physiological analysis, markerassisted selection, and transgenic technology.

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Introgression of a novel salt‐tolerant L‐myo‐inositol 1‐phosphate synthase from Porteresia coarctata (Roxb.) Tateoka (PcINO1) confers salt tolerance to evolutionary diverse organisms

Cited by 118 publications

References 34 publications

Upgrading Root Physiology for Stress Tolerance by Ectomycorrhizas: Insights from Metabolite and Transcriptional Profiling into Reprogramming for Stress Anticipation

Upgrading Root Physiology for Stress Tolerance by Ectomycorrhizas: Insights from Metabolite and Transcriptional Profiling into Reprogramming for Stress Anticipation

Functional identification of sll1383 from Synechocystis sp PCC 6803 as L-myo-inositol 1-phosphate phosphatase (EC 3.1.3.25): molecular cloning, expression and characterization

Approaches to Increasing Salt Tolerance in Crop Plants

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